Yeast carboxypeptidase Y vacuolar targeting signal is defined by four propeptide amino acids.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Intracellular Aspects of the Process of Protein Synthesis

1975 • 3,560 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

A C-terminal signal prevents secretion of luminal ER proteins

1987 • 2,189 citations

Signal sequences

1985 • 2,179 citations

The Biogenesis of Lysosomes

1989 • 1,583 citations

Intracellular protein topogenesis

1980 • 1,378 citations

A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

1976 • 1,313 citations

Constitutive and Regulated Secretion of Proteins

1987 • 1,021 citations

BIOSYNTHETIC PROTEIN TRANSPORT AND SORTING BY THE ENDOPLASMIC RETICULUM AND GOLGI

1987 • 985 citations

Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases.

1988 • 977 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

1979 • 967 citations

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA

1985 • 849 citations

Control of Protein Exit from the Endoplasmic Reticulum

1989 • 794 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

The rate of bulk flow from the endoplasmic reticulum to the cell surface

1987 • 542 citations

Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment.

1988 • 434 citations

The Phosphomannosyl Recognition System for Intracellular and Intercellular Transport of Lysosomal Enzymes

1982 • 379 citations

Protein Sorting in Saccharomyces cerevisiae: Isolation of Mutants Defective in the Delivery and Processing of Multiple Vacuolar Hydrolases

1988 • 339 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease

1987 • 312 citations

Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information.

1988 • 287 citations

Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae.

1989 • 258 citations

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA

1985 • 247 citations

Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y.

1986 • 217 citations

Mutant defective in processing of an enzyme located in the lysosome-like vacuole of Saccharomyces cerevisiae.

1981 • 199 citations

Protein sorting by selective retention in the endoplasmic reticulum and Golgi stack

1987 • 179 citations

Biosynthesis of the Vacuolar Yeast Glycoprotein Carboxypeptidase Y

1978 • 164 citations

Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.

1987 • 161 citations

Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells.

1989 • 154 citations

Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.

1986 • 151 citations

Lysosomal enzyme phosphorylation. Recognition of a protein-dependent determinant allows specific phosphorylation of oligosaccharides present on lysosomal enzymes.

1984 • 150 citations

Intracellular Sorting and Processing of a Yeast Vacuolar Hydrolase: Proteinase A Propeptide Contains Vacuolar Targeting Information

1988 • 121 citations

A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.

1986 • 114 citations

Protein targeting to the yeast vacuole

1989 • 81 citations

Yeast carboxypeptidase Y can be translocated and glycosylated without its amino-terminal signal sequence.

1987 • 80 citations

Protease B of the Lysosomelike Vacuole of the Yeast Saccharomyces Cerevisiae Is Homologous to the Subtilisin Family of Serine Proteases

1987 • 38 citations

Increased hydrophobicity of the S′1 binding site in carboxypeptidase Y obtained by site-directed mutagenesis

1985 • 19 citations