PEP4 Gene of Saccharomyces cerevisiae Encodes Proteinase A, a Vacuolar Enzyme Required for Processing of Vacuolar Precursors

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Signal sequences

1985 • 2,179 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

The molecular biology of the yeast saccharomyces: Metabolism and gene expression

1983 • 1,118 citations

Compilation of published signal sequences

1984 • 784 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

Lysosomes in biology and pathology

1975 • 511 citations

Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

1980 • 502 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Cloning and sequence analysis of cDNA for human cathepsin D.

1985 • 318 citations

Molecular structure of an aspartic proteinase zymogen, porcine pepsinogen, at 1.8 Å resolution

1986 • 295 citations

Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y.

1986 • 217 citations

PEP4 GENE FUNCTION IS REQUIRED FOR EXPRESSION OF SEVERAL VACUOLAR HYDROLASES IN SACCHAROMYCES CEREVISIAE

1982 • 211 citations

Mutant defective in processing of an enzyme located in the lysosome-like vacuole of Saccharomyces cerevisiae.

1981 • 199 citations

Biosynthesis of a lysosomal enzyme. Partial structure of two transient and functionally distinct NH2-terminal sequences in cathepsin D.

1981 • 188 citations

THE SYNTHESIS AND FUNCTION OF PROTEASES IN SACCHAROMYCES: GENETIC APPROACHES

1984 • 179 citations

Biosynthesis of the Vacuolar Yeast Glycoprotein Carboxypeptidase Y

1978 • 164 citations

Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene.

1986 • 151 citations

PROTEIN DEGRADATION, MEIOSIS AND SPORULATION IN PROTEINASE-DEFICIENT MUTANTS OF SACCHAROMYCES CEREVISIAE

1981 • 115 citations

Evolution in the structure and function of carboxyl proteases

1979 • 105 citations

Expression of the BAR1 gene in Saccharomyces cerevisiae: induction by the alpha mating pheromone of an activity associated with a secreted protein

1983 • 100 citations

GENETIC PROPERTIES OF MUTATIONS AT THE PEP4 LOCUS IN SACCHAROMYCES CEREVISIAE

1982 • 93 citations

KINETICS OF THE FORMATION OF PEPSIN FROM SWINE PEPSINOGEN AND IDENTIFICATION OF AN INTERMEDIATE COMPOUND

1938 • 92 citations

In vivo biosynthesis of the vacuolar proteinases A and B in the yeast Saccharomyces cerevisiae.

1982 • 92 citations

Intramolecular Activation of Porcine Pepsinogen

1971 • 89 citations

Genes required for completion of import of proteins into the endoplasmic reticulum in yeast.

1984 • 86 citations

Mutations in PEP4 locus of Saccharomyces cerevisiae block final step in maturation of two vacuolar hydrolases.

1983 • 84 citations

Purification and properties of proteinase A from yeast.

1980 • 84 citations

Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae

1983 • 83 citations

Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated.

1981 • 82 citations

Analysis of Proteinase A Function in Yeast

1981 • 58 citations

Primary structure of the aspartic proteinase A from Saccharomyces cerevisiae

1986 • 57 citations

Invivo biosynthesis of vacuolar proteinases in proteinase mutants of Saccharomycescerevisiae

1982 • 42 citations

Isolation of yeast mutants lacking proteinase B activity

1978 • 36 citations

A particulate form of alkaline phosphatase in the yeast, Saccharomyces cerevisiae

1981 • 26 citations