Structural domains and molecular lifestyles of insulin and its precursors in the pancreatic Beta cell

Intracellular Aspects of the Process of Protein Synthesis

1975 • 3,560 citations

Signal sequences

1985 • 2,179 citations

The trans Golgi Network: Sorting at the Exit Site of the Golgi Complex

1986 • 1,175 citations

Pathways of Protein Secretion in Eukaryotes

1985 • 1,050 citations

BIOSYNTHETIC PROTEIN TRANSPORT AND SORTING BY THE ENDOPLASMIC RETICULUM AND GOLGI

1987 • 985 citations

Polypeptide chain binding proteins: Catalysts of protein folding and related processes in cells

1989 • 884 citations

Insulin: The Structure in the Crystal and its Reflection in Chemistry and Biology by

1972 • 831 citations

β-cells in type II diabetes mellitus

1991 • 582 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast

1990 • 578 citations

Mechanism of Protein Translocation Across the Endoplasmic Reticulum Membrane

1986 • 509 citations

Monomeric insulins obtained by protein engineering and their medical implications

1988 • 493 citations

Degradation from the endoplasmic reticulum: Disposing of newly synthesized proteins

1988 • 488 citations

Protein disulfide isomerase: Multiple roles in the modification of nascent secretory proteins

1989 • 466 citations

Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.

1991 • 449 citations

Intraorganellar calcium and pH control proinsulin cleavage in the pancreatic β cell via two distinct site-specific endopeptidases

1988 • 403 citations

A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein β-adaptin

1991 • 376 citations

Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles

1987 • 335 citations

Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles.

1986 • 331 citations

Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation

1983 • 329 citations

The primary structure of human secretogranin II, a widespread tyrosine-sulfated secretory granule protein that exhibits low pH- and calcium-induced aggregation

1989 • 309 citations

Macro- and Micro-Domains in the Endocrine Pancreas

1982 • 306 citations

Cell-free protein sorting to the regulated and constitutive secretory pathways

1990 • 289 citations

Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein

1989 • 288 citations

The trans-most cisternae of the Golgi complex: A compartment for sorting of secretory and plasma membrane proteins

1987 • 275 citations

Disproportionate elevation of immunoreactive proinsulin in Type 2 (non-insulin-dependent) diabetes mellitus and in experimental insulin resistance

1987 • 274 citations

Insulin-Resistant Diabetes Due to a Point Mutation That Prevents Insulin Proreceptor Processing

1988 • 253 citations

Role of zinc in insulin biosynthesis

1980 • 251 citations

Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export

1989 • 222 citations

Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein.

1986 • 207 citations

Newly synthesized proinsulin/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway.

1987 • 204 citations

The relationships of concentrations of insulin, intact proinsulin and 32?33 split proinsulin with cardiovascular risk factors in Type 2 (non-insulin-dependent) diabetic subjects

1990 • 198 citations

The insulin secretory granule

1989 • 188 citations

Hyperproinsulinemia and Amyloid in NIDDM: Clues to Etiology of Islet β-Cell Dysfunction?

1989 • 186 citations

Precursors for peptide hormones share common secondary structures forming features at the proteolytic processing sites

1986 • 183 citations

Heat shock and the sorting of luminal ER proteins.

1989 • 178 citations

Serum proinsulin levels at fasting and after oral glucose load in patients with Type 2 (non-insulin-dependent) diabetes mellitus

1988 • 176 citations

Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone in Mammalian Cells

1988 • 173 citations

The insulin factory: a tour of the plant surroundings and a visit to the assembly line

1985 • 172 citations

Intracellular degradation of insulin stores by rat pancreatic islets in vitro. An alternative pathway for homeostasis of pancreatic insulin content.

1980 • 168 citations

Molecular Sorting in the Secretory Pathway

1989 • 155 citations

Lessons Learned From Molecular Biology of Insulin-Gene Mutations

1990 • 154 citations

Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells.

1989 • 154 citations

Purification of three related peripheral membrane proteins needed for vesicular transport.

1990 • 147 citations

The insulin-secretory-granule carboxypeptidase H. Purification and demonstration of involvement in proinsulin processing

1987 • 143 citations

A mutant human proinsulin is secreted from islets of Langerhans in increased amounts via an unregulated pathway.

1988 • 135 citations

Insulin, not C-peptide (proinsulin), is present in crinophagic bodies of the pancreatic B-cell.

1984 • 132 citations

A superactive insulin: [B10-aspartic acid]insulin(human).

1987 • 129 citations

A circulating variant of human proalbumin

1978 • 124 citations

Direct measurement of plasma proinsulin in normal and diabetic subjects

1972 • 101 citations

Circulating proinsulin in patients with maturity onset diabetes

1977 • 100 citations

NMR and photo-CIDNP studies of human proinsulin and prohormone processing intermediates with application to endopeptidase recognition

1990 • 99 citations

Studies on the molecular organization of rat insulin secretory granules.

1987 • 95 citations

Familial Hyperproinsulinemia Due to a Proposed Defect in Conversion of Proinsulin to Insulin

1984 • 94 citations

Proinsulin Secretion by a Pancreatic Beta-Cell Adenoma

1970 • 93 citations

The rate of bulk flow from the Golgi to the plasma membrane

1990 • 91 citations

Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway.

1990 • 80 citations

Recognition of nascent polypeptides for targeting and folding

1991 • 78 citations

Protein transport across the ER membrane

1990 • 75 citations

Regulation of Insulin-Gene Expression

1987 • 73 citations

Stimulation by ATP of proinsulin to insulin conversion in isolated rat pancreatic islet secretory granules. Association with the ATP-dependent proton pump.

1987 • 72 citations

Calcium‐dependent KEX2‐like protease found in hepatic secretory vesicles converts proalbumin to albumin

1988 • 72 citations

Proinsulin-like component of circulating insulin in the basal state and in patients and hamsters with islet cell tumors

1971 • 70 citations

Proton-translocating Mg2+-dependent ATPase activity in insulin-secretory granules

1982 • 67 citations

Efficient targeting to storage granules of human proinsulins with altered propeptide domain.

1988 • 64 citations

Processing Endoprotease Recognizes a Structural Feature at the Cleavage Site of Peptide Prohormones

1989 • 62 citations

Role of microtubules in the synthesis, conversion, and release of (pro)insulin. A biochemical and radioautographic study in rat islets.

1979 • 58 citations

Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment.

1984 • 55 citations

Partial diversion of a mutant proinsulin (B10 aspartic acid) from the regulated to the constitutive secretory pathway in transfected AtT-20 cells.

1989 • 53 citations

Role of ATP in the Intracellular Translocation of Proinsulin and Insulin in the Rat Pancreatic B Cell

1972 • 53 citations

Intracellular transport and sorting of mutant human proinsulins that fail to form hexamers.

1991 • 51 citations

Proinsulin endopeptidase substrate specificities defined by site-directed mutagenesis of proinsulin

1989 • 50 citations

A motif found in propeptides and prohormones that may target them to secretory vesicles

1991 • 49 citations

(Pro)insulin associates with Golgi membranes of pancreatic B cells.

1984 • 43 citations

The tissue-specific processing of Pro-ACTH/Endorphin recent advances and unsolved problems

1990 • 42 citations

Deletion of a highly conserved tetrapeptide sequence of the proinsulin connecting peptide (C-peptide) inhibits proinsulin to insulin conversion by transfected pituitary corticotroph (AtT20) cells

1989 • 41 citations

Resistance of the insulin crystal to lysosomal proteases: implications for pancreatic B-cell crinophagy

1987 • 40 citations

Relative Hypersecretion of Proinsulin in Rat Model of NIDDM

1991 • 35 citations

Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II

1991 • 31 citations

Molecular dissection of early stages of the eukaryotic secretory pathway

1990 • 30 citations

Inhibition of proinsulin to insulin conversion in rat islets using arginine and lysine analogs. Lack of effect on rate of release of modified products.

1982 • 30 citations

Mutations in Signal Sequence Cleavage Domain of Preproparathyroid Hormone Alter Protein Translocation, Signal Sequence Cleavage, and Membrane-Binding Properties

1989 • 27 citations

An in vivo characterization of the cleavage site specificity of the insulin cell prohormone processing enzymes.

1990 • 27 citations

Influence of Glucose on Insulin Handling by Rat Islets in Culture: A Reflection of Integrated Changes in Insulin Biosynthesis, Release, and Intracellular Degradation

1983 • 23 citations

The intracellular handling of insulin-related peptides in isolated pancreatic islets. Evidence for differential rates of degradation of insulin and C-peptide

1988 • 23 citations

Glucose regulation of insulin gene expression.

1989 • 21 citations

Contrasting Patterns of Insulin Biosynthesis, Compartmental Storage, and Secretion: Rat Tumor Versus Islet Cells

1984 • 18 citations

Differential kinetics of rat insulin I and II processing in rat islets of Langerhans

1987 • 17 citations

Heterogeneity of Expression and Secretion of Native and Mutant [Asp^B10]lnsulin in AtT20 Cells

1991 • 16 citations

Proinsulin trafckin and processing in the pancreatic B cell

1990 • 15 citations

Role of Microtubules in the Synthesis, Conversion, and Release of (Pro)Insulin

1979 • 10 citations

Proinsulin modified by analogues of arginine and lysine is degraded rapidly in pancreatic B-cells

1984 • 8 citations