Role of Hydrophobic Amino Acid Clusters in the Transactivation Activity of the Human Glucocorticoid Receptor

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

The Steroid and Thyroid Hormone Receptor Superfamily

1988 • 7,590 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Sequence and Characterization of a Coactivator for the Steroid Hormone Receptor Superfamily

1995 • 2,390 citations

A CBP Integrator Complex Mediates Transcriptional Activation and AP-1 Inhibition by Nuclear Receptors

1996 • 2,183 citations

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme

1989 • 1,244 citations

A Thermodynamic Scale for the Helix-Forming Tendencies of the Commonly Occurring Amino Acids

1990 • 1,173 citations

Functional domains of the human glucocorticoid receptor

1986 • 933 citations

Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors.

1992 • 829 citations

Nuclear factor RIP140 modulates transcriptional activation by the estrogen receptor.

1995 • 756 citations

Multiple and cooperative trans-activation domains of the human glucocorticoid receptor

1988 • 705 citations

GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.

1996 • 702 citations

Estrogen Receptor-Associated Proteins: Possible Mediators of Hormone-Induced Transcription

1994 • 645 citations

Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein.

1994 • 644 citations

Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

1990 • 620 citations

A glutamine-rich hydrophobic patch in transcription factor Sp1 contacts the dTAFII110 component of the Drosophila TFIID complex and mediates transcriptional activation.

1994 • 531 citations

Many Transcription Factors Interact Synergistically with Steroid Receptors

1988 • 517 citations

The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assembly

1991 • 497 citations

Critical Structural Elements of the VP16 Transcriptional Activation Domain

1991 • 461 citations

Acid blobs and negative noodles

1988 • 438 citations

COMMON THEMES IN ASSEMBLY AND FUNCTION OF EUKARYOTIC TRANSCRIPTION COMPLEXES

1995 • 434 citations

Applications of high efficiency lithium acetate transformation of intact yeast cells using single‐stranded nucleic acids as carrier

1991 • 433 citations

Differential ligand-dependent interactions between the AF-2 activating domain of nuclear receptors and the putative transcriptional intermediary factors mSUG1 and TIF1.

1996 • 410 citations

Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor

1994 • 402 citations

Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor

1991 • 381 citations

Interaction of proteins with transcriptionally active estrogen receptors.

1994 • 379 citations

Binding of general transcription factor TFIIB to an acidic activating region

1991 • 377 citations

A DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression.

1987 • 360 citations

Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.

1994 • 355 citations

Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II).

1992 • 354 citations

Interaction of human thyroid hormone receptor beta with transcription factor TFIIB may mediate target gene derepression and activation by thyroid hormone.

1993 • 328 citations

Hydrophilicity of polar amino acid side-chains is markedly reduced by flanking peptide bonds

1988 • 296 citations

Unliganded thyroid hormone receptor inhibits formation of a functional preinitiation complex: implications for active repression.

1993 • 262 citations

Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator.

1993 • 261 citations

TBP-TAF complexes: selectivity factors for eukaryotic transcription

1994 • 250 citations

The Zta trans-activator protein stabilizes TFIID association with promoter DNA by direct protein-protein interaction.

1991 • 249 citations

A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene

1984 • 237 citations

Transcriptional control by nuclear receptors

1991 • 234 citations

Transcription factor TFIIB and the vitamin D receptor cooperatively activate ligand-dependent transcription.

1995 • 212 citations

Direct interaction between the transcriptional activation domain of human p53 and the TATA box-binding protein.

1993 • 209 citations

Identification of phosphorylated sites in the mouse glucocorticoid receptor.

1991 • 208 citations

Protein-protein interaction via PAS domains: role of the PAS domain in positive and negative regulation of the bHLH/PAS dioxin receptor-Arnt transcription factor complex.

1995 • 187 citations

A protein that interacts with members of the nuclear hormone receptor family: identification and cDNA cloning.

1995 • 186 citations

Rapid protein extraction from Saccharomyces cerevisiae

1994 • 167 citations

The Vitamin D Receptor Interacts with General Transcription Factor IIB

1995 • 166 citations

Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor.

1993 • 161 citations

Structural characterization of a minimal functional transactivation domain from the human glucocorticoid receptor.

1995 • 160 citations

Structural and functional analysis of the NF-kappa B p65 C terminus. An acidic and modular transactivation domain with the potential to adopt an alpha-helical conformation.

1994 • 158 citations

Interaction of the COOH-terminal Transactivation Domain of p65 NF-κB with TATA-binding Protein, Transcription Factor IIB, and Coactivators

1995 • 157 citations

The Transcriptional Activator GCN4 Contains Multiple Activation Domains That Are Critically Dependent on Hydrophobic Amino Acids

1995 • 156 citations

The acidic activation domains of the GCN4 and GAL4 proteins are not α helical but form β sheets

1993 • 151 citations

Transcriptional coactivators in yeast and beyond

1995 • 146 citations

転写因子TF II Bとヒト甲状腺ホルモン受容体βとの結合は標的遺伝子の発現と甲状腺ホルモンによる活性化を仲介する

1993 • 135 citations

Regulation of gene expression by nuclear hormone receptors

1989 • 133 citations

Efficient site-directed in vitro mutagenesis using phagemid vectors.

1989 • 118 citations

Functional Interaction of the c-Myc Transactivation Domain with the TATA Binding Protein: Evidence for an Induced Fit Model of Transactivation Domain Folding

1996 • 109 citations

Role of Acidic and Phosphorylated Residues in Gene Activation by the Glucocorticoid Receptor

1995 • 107 citations

In Vitro Transcription Enhancement by Purified Derivatives of the Glucocorticoid Receptor

1989 • 107 citations

Single-step purification of shuttle vectors from yeast for high frequency back-transformation intoE. coli

1990 • 105 citations

Mutational analysis of the transcription activation domain of RelA: identification of a highly synergistic minimal acidic activation module.

1994 • 104 citations

Delineation of a small region within the majortransactivation domain of the human glucocorticoid receptor that mediatestransactivation of gene expression.

1994 • 98 citations

Site-directed mutagenesis of the phosphorylation sites in the mouse glucocorticoid receptor.

1993 • 96 citations

Transcriptional Activation Domain of the Herpesvirus Protein VP16 Becomes Conformationally Constrained upon Interaction with Basal Transcription Factors

1996 • 89 citations

Interaction with RAP74 subunit of TFIIF is required for transcriptional activation by serum response factor

1995 • 89 citations

Identification of Seven Hydrophobic Clusters in GCN4 Making Redundant Contributions to Transcriptional Activation

1996 • 86 citations

Recombinant human glucocorticoid receptor induces transcription of hormone response genes in vitro.

1990 • 78 citations

Ligand-specific transactivation of gene expression by a derivative of the human glucocorticoid receptor expressed in yeast.

1990 • 77 citations

Direct interaction of the tau 1 transactivation domain of the human glucocorticoid receptor with the basal transcriptional machinery.

1993 • 63 citations

A Fraction Enriched in a Novel Glucocorticoid Receptor-interacting Protein Stimulates Receptor-dependent Transcription in Vitro

1995 • 58 citations

Identification of a Transactivation Function in the Progesterone Receptor That Interacts with the TAFII110 Subunit of the TFIID Complex

1995 • 57 citations

Structural Studies of Mutant Glucocorticoid Receptor Transactivation Domains Establish a Link between Transactivation Activity in Vivo and α-Helix-Forming Potential in Vitro

1996 • 43 citations

High Level Expression of the Major Transactivation Domain of the Human Glucocorticoid Receptor in Yeast Cells Inhibits Endogenous Gene Expression and Cell Growth

1991 • 43 citations

A Direct Interaction between a Glutamine-Rich Activator and the N Terminus of TFIIB Can Mediate Transcriptional Activation In Vivo

1995 • 39 citations

The glucocorticoid receptor functions at multiple steps during transcription initiation by RNA polymerase II.

1994 • 30 citations

Identification of single amino acid substitutions of Cys-736 that affect the steroid-binding affinity and specificity of the glucocorticoid receptor using phenotypic screening in yeast.

1996 • 26 citations

Activation Function 1 of Retinoic Acid Receptor β2 Is an Acidic Activator Resembling VP16

1995 • 23 citations

Spectroscopic studies of a transcriptional activation peptide

1992 • 7 citations