The Transcriptional Activator GCN4 Contains Multiple Activation Domains That Are Critically Dependent on Hydrophobic Amino Acids

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in Yeast Genetics

1988 • 4,905 citations

The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding Proteins

1988 • 3,479 citations

X-Ray Structure of the GCN4 Leucine Zipper, a Two-Stranded, Parallel Coiled Coil

1991 • 1,433 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

Transcriptional activation: A complex puzzle with few easy pieces

1994 • 1,112 citations

A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II

1994 • 1,064 citations

The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted α Helices: Crystal structure of the protein-DNA complex

1992 • 964 citations

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast

1986 • 946 citations

Deletion analysis of GAL4 defines two transcriptional activating segments

1987 • 898 citations

Transcription activation by the adenovirus E1a protein

1989 • 667 citations

Multiple upstream AUG codons mediate translational control of GCN4

1986 • 654 citations

Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein.

1994 • 644 citations

Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

1990 • 620 citations

Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators

1993 • 589 citations

An RNA polymerase II holoenzyme responsive to activators

1994 • 586 citations

Activators and targets

1990 • 582 citations

Mechanism of action of an acidic transcriptional activator in vitro

1991 • 557 citations

A glutamine-rich hydrophobic patch in transcription factor Sp1 contacts the dTAFII110 component of the Drosophila TFIID complex and mediates transcriptional activation.

1994 • 531 citations

Wild-type p53 binds to the TATA-binding protein and represses transcription.

1992 • 527 citations

Critical Structural Elements of the VP16 Transcriptional Activation Domain

1991 • 461 citations

Genetic isolation of ADA2: A potential transcriptional adaptor required for function of certain acidic activation domains

1992 • 446 citations

The structure of transposable yeast mating type loci

1980 • 439 citations

Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB

1993 • 418 citations

New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacA gene sequences encoding an enzymatically active carboxy-terminal portion of β-galactosidase

1983 • 416 citations

The basics of basal transcription by RNA polymerase II

1994 • 409 citations

GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA.

1987 • 395 citations

Binding of general transcription factor TFIIB to an acidic activating region

1991 • 377 citations

Reduced binding of TFIID to transcriptionally compromised mutants of VP16

1991 • 362 citations

Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein

1988 • 325 citations

Two distinct yeast transcriptional activators require the function of the GCN5 protein to promote normal levels of transcription.

1992 • 302 citations

Positive regulation in the general amino acid control of Saccharomyces cerevisiae.

1983 • 279 citations

Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator.

1993 • 261 citations

Multiple Global Regulators Control HIS4 transcription in Yeast

1987 • 246 citations

Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation

1993 • 225 citations

Association of RAP1 binding sites with stringent control of ribosomal protein gene transcription in Saccharomyces cerevisiae.

1991 • 181 citations

The cross-pathway control gene of Neurospora crassa, cpc-1, encodes a protein similar to GCN4 of yeast and the DNA-binding domain of the oncogene v-jun-encoded protein.

1988 • 172 citations

7 General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae

1992 • 167 citations

The acidic activation domains of the GCN4 and GAL4 proteins are not α helical but form β sheets

1993 • 151 citations

Nondissociation of GAL4 and GAL80 in Vivo After Galactose Induction

1992 • 139 citations

Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet

1993 • 138 citations

Coupling of GCN4 mRNA translational activation with decreased rates of polypeptide chain initiation

1989 • 137 citations

Structure(?) and function of acidic transcription activators

1993 • 136 citations

The zinc finger region of the adenovirus E1A transactivating domain complexes with the TATA box binding protein.

1994 • 123 citations

Promega protocols and applications guide

1996 • 114 citations

The Epstein-Barr virus R transactivator (Rta) contains a complex, potent activation domain with properties different from those of VP16

1992 • 113 citations

Effects of activation-defective TBP mutations on transcription initiation in yeast

1994 • 110 citations

Truncated protein phosphatase GLC7 restores translational activation of GCN4 expression in yeast mutants defective for the eIF-2 alpha kinase GCN2.

1992 • 104 citations

Interaction between the N-terminal domain of the 230-kDa subunit and the TATA box-binding subunit of TFIID negatively regulates TATA-box binding.

1994 • 78 citations

c-Fos-induced activation of a TATA-box-containing promoter involves direct contact with TATA-box-binding protein.

1994 • 72 citations

Promega: Protocols and Applications Guide

1996 • 61 citations

Multicopy tRNA Genes Functionally Suppress Mutations in Yeast eIF-2α Kinase GCN2: Evidence for Separate Pathways Coupling GCN4 Expression to Uncharged tRNA

1994 • 54 citations

TATA-dependent and TATA-independent transcription at the HIS4 gene of yeast

1990 • 43 citations

The positive regulatory function of the 5'-proximal open reading frames in GCN4 mRNA can be mimicked by heterologous, short coding sequences.

1988 • 38 citations

Arginine restriction induced by delta-N-(phosphonacetyl)-L-ornithine signals increased expression of HIS3, TRP5, CPA1, and CPA2 in Saccharomyces cerevisiae.

1989 • 17 citations