Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Genomic sequencing.

1984 • 8,343 citations

A Receptor-Mediated Pathway for Cholesterol Homeostasis

1986 • 5,638 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

Fluorescence detection in automated DNA sequence analysis

1986 • 1,694 citations

[19] Receptor-mediated endocytosis of low-density lipoprotein in cultured cells

1983 • 1,639 citations

P450 GENES: STRUCTURE, EVOLUTION, AND REGULATION

1987 • 1,559 citations

Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth.

1980 • 1,195 citations

Plasmid screening at high colony density

1980 • 1,100 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

1983 • 933 citations

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

1985 • 818 citations

Mutation in LDL Receptor: Alu-Alu Recombination Deletes Exons Encoding Transmembrane and Cytoplasmic Domains

1985 • 453 citations

Mitochondrial presequences.

1988 • 357 citations

Deletion in Cysteine-Rich Region of LDL Receptor Impedes Transport to Cell Surface in WHHL Rabbit

1986 • 335 citations

Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.

1984 • 296 citations

Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence

1987 • 284 citations

Mutational analysis of the ligand binding domain of the low density lipoprotein receptor.

1988 • 281 citations

Alu-Alu recombination deletes splice acceptor sites and produces secreted low density lipoprotein receptor in a subject with familial hypercholesterolemia.

1987 • 228 citations

Cerebrotendinous xanthomatosis: a defect in mitochondrial 26-hydroxylation required for normal biosynthesis of cholic acid.

1980 • 221 citations

42 bp element from LDL receptor gene confers end-product repression by sterols when inserted into viral TK promoter

1987 • 220 citations

5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription

1985 • 187 citations

Three direct repeats and a TATA-like sequence are required for regulated expression of the human low density lipoprotein receptor gene.

1987 • 180 citations

Targeting proteins into mitochondria

1986 • 171 citations

Coding nucleotide sequence of rat NADPH-cytochrome P-450 oxidoreductase cDNA and identification of flavin-binding domains.

1985 • 171 citations

Multiple sterol regulatory elements in promoter for hamster 3-hydroxy-3-methylglutaryl-coenzyme A synthase.

1988 • 165 citations

ω‐Hydroxylation of Steroid Side‐Chain in Biosynthesis of Bile Acids

1973 • 130 citations

Hydroxylations in biosynthesis of bile acids. Isolation of a cytochrome P-450 from rabbit liver mitochondria catalyzing 26-hydroxylation of C27-steroids.

1984 • 127 citations

Molecular cloning and amino acid sequence of the precursor form of bovine adrenodoxin: evidence for a previously unidentified COOH-terminal peptide.

1985 • 120 citations

Demonstration of 26-hydroxylation of C27-steroids in human skin fibroblasts, and a deficiency of this activity in cerebrotendinous xanthomatosis.

1986 • 106 citations

Cholic Acid Biosynthesis

1979 • 103 citations

Simultaneous transfection of COS-1 cells with mitochondrial and microsomal steroid hydroxylases: incorporation of a steroidogenic pathway into nonsteroidogenic cells.

1988 • 77 citations

25-Hydroxylation of vitamin D3 by a cytochrome P-450 from rabbit liver mitochondria

1988 • 74 citations

Expression of a functional 78,000 dalton mammalian flavoprotein, NADPH-cytochrome P-450 oxidoreductase, in Escherichia coli

1987 • 74 citations

Sex differences in cytochrome P-450-dependent 25-hydroxylation of C27-steroids and vitamin D3 in rat liver microsomes.

1986 • 68 citations

Hepatic mitochondrial cytochrome P -450: Isolation and functional characterization

1977 • 66 citations

Evidence that the 5′-untranslated leader of mRNA affects the requirement for wheat germ initiation factors 4A, 4F, and 4G.

1988 • 54 citations

Regulation of Low Density Lipoprotein Receptor Gene Expression in Human Lymphocytes

1989 • 47 citations

Mechanism of androstenedione formation from testosterone and epitestosterone catalyzed by purified cytochrome P-450b.

1988 • 46 citations

[21] Purification from rabbit and rat liver of cytochromes P-450 involved in bile acid biosynthesis

1985 • 42 citations

Primary structure of glycolate oxidase from spinach

1988 • 37 citations

Characterization of the liver mitochondrial cytochrome P-450 catalyzing the 26-hydroxylation of 5β-cholestane-3α,7α,12α-triol

1988 • 31 citations

Conversion of 5β-cholestane-3α,7α,12α,26-tetrol into 3α,7α,12α-trihydroxy-5β-cholestanoic acid by rabbit liver mitochondria

1988 • 11 citations