A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

1977 • 4,696 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Yeast/E. coli shuttle vectors with multiple unique restriction sites

1986 • 1,399 citations

Functional inactivation of genes by dominant negative mutations

1987 • 1,174 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene

1980 • 511 citations

GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeast

1985 • 496 citations

Mitotic stability of yeast chromosomes: A colony color assay that measures nondisjunction and chromosome loss

1985 • 486 citations

Structure and function of the yeast URA3 gene: expression in Escherichia coli

1984 • 311 citations

Plasmid vector pBR322 and its special-purpose derivatives — a review

1986 • 311 citations

One-Step Gene Disruption in Yeast

1989 • 310 citations

Centromeric DNA from Saccharomyces cerevisiae

1982 • 292 citations

Nucleotide sequence and transcriptional mapping of the yeastpet56-his3-dedlgene region

1985 • 290 citations

Vector systems for the expression, analysis and cloning of DNA sequence in S. cerevisiae

1985 • 265 citations

Functional selection and analysis of yeast centromeric DNA

1985 • 249 citations

A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae

1988 • 221 citations

Nucleotide sequence of yeast LEU2 shows 5′-noncoding region has sequences cognate to leucine

1982 • 208 citations

Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences

1985 • 157 citations

Yeast LEU2. Repression of mRNA levels by leucine and primary structure of the gene product.

1984 • 152 citations

Mutational analysis of centromere DNA from chromosome VI of Saccharomyces cerevisiae.

1988 • 148 citations

Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene

1984 • 140 citations

DNA sequences of yeast H3 and H4 histone genes from two non-allelic gene sets encode identical H3 and H4 proteins

1983 • 139 citations

Fine-structure analysis of the DNA sequence requirements for autonomous replication of Saccharomyces cerevisiae plasmids.

1986 • 113 citations

Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains.

1982 • 111 citations

Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.

1988 • 94 citations

Deletion analysis of the Saccharomyces GAL gene cluster

1981 • 68 citations

[22] Visual assay for chromosome ploidy

1987 • 67 citations

Expression inSaccharomyces cerevisiaeof human interferon-alpha directed by theTRP1 5′region

1983 • 54 citations

Direction of chromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational substrates.

1988 • 44 citations

Two related regulatory sequences are required for maximal induction of Saccharomyces cerevisiae his3 transcription.

1987 • 37 citations