The acidic activation domains of the GCN4 and GAL4 proteins are not α helical but form β sheets

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Computed circular dichroism spectra for the evaluation of protein conformation

1969 • 3,654 citations

Spectroscopic Determination of Tryptophan and Tyrosine in Proteins^*

1967 • 3,423 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

How eukaryotic transcriptional activators work

1988 • 1,924 citations

Deletion analysis of GAL4 defines two transcriptional activating segments

1987 • 898 citations

Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.

1988 • 830 citations

A new class of yeast transcriptional activators

1987 • 706 citations

Negative effect of the transcriptional activator GAL4

1988 • 672 citations

Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

1990 • 620 citations

Mechanism of action of an acidic transcriptional activator in vitro

1991 • 557 citations

Critical Structural Elements of the VP16 Transcriptional Activation Domain

1991 • 461 citations

Acid blobs and negative noodles

1988 • 438 citations

Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor

1991 • 381 citations

The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80

1987 • 349 citations

Commitment and activation at pol II promoters: A tail of protein-protein interactions

1990 • 332 citations

Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein

1988 • 325 citations

Transcription in yeast activated by a putative amphipathic α helix linked to a DNA binding unit

1987 • 324 citations

Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator.

1993 • 261 citations

Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.

1991 • 242 citations

Interaction of positive and negative regulatory proteins in the galactose regulon of yeast

1987 • 226 citations

Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4

1992 • 196 citations

Design, synthesis, and characterization of a cytotoxic peptide with melittin-like activity

1981 • 165 citations

The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.

1992 • 160 citations

Analysis of theKluyveromyces lactispositive regulatory geneLAC9reveals functional homology to, but sequence divergence from, theSaccharomyces cerevisiae GALAgene

1986 • 158 citations

Nondissociation of GAL4 and GAL80 in Vivo After Galactose Induction

1992 • 139 citations

Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet

1993 • 138 citations

Purification and characterization of the carboxyl-terminal transactivation domain of Vmw65 from herpes simplex virus type 1.

1992 • 93 citations

Structural studies of the acidic transactivation domain of the Vmw65 protein of herpes simplex virus using proton NMR

1992 • 72 citations

GAL4 Protein: Purification, Association with GAL80 Protein, and Conserved Domain Structure

1990 • 72 citations

Yeast GCN4 transcriptional activator protein interacts with RNA polymerase II in vitro.

1989 • 41 citations

Synthesis of Peptides as Cloned Ubiquitin Extensions

1989 • 36 citations

Interaction between transcriptional activator protein LAC9 and negative regulatory protein GAL80.

1989 • 35 citations

GAL4 protein: purification, association with GAL80 protein, and conserved domain structure.

1990 • 34 citations

Spectroscopic studies of a transcriptional activation peptide

1992 • 7 citations