Structure, function, and mechanism of the Hsp90 molecular chaperone

Hsp90 as a capacitor for morphological evolution

1998 • 2,261 citations

Active and Inactive Protein Kinases: Structural Basis for Regulation

1996 • 1,361 citations

Crystal Structure of an Hsp90–Geldanamycin Complex: Targeting of a Protein Chaperone by an Antitumor Agent

1997 • 1,345 citations

Identification and Structural Characterization of the ATP/ADP-Binding Site in the Hsp90 Molecular Chaperone

1997 • 1,235 citations

Structure of TPR Domain–Peptide Complexes

2000 • 1,159 citations

The 90-kDa Molecular Chaperone Family

1998 • 1,008 citations

Structural Basis for Inhibition of the Hsp90 Molecular Chaperone by the Antitumor Antibiotics Radicicol and Geldanamycin

1999 • 979 citations

An atypical topoisomerase II from archaea with implications for meiotic recombination

1997 • 890 citations

The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions

1998 • 820 citations

ATP binding and hydrolysis are essential to the function of the Hsp90 molecular chaperone invivo

1998 • 709 citations

Hsp90 & Co. – a holding for folding

1999 • 631 citations

hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.

1989 • 624 citations

In Vivo Function of Hsp90 Is Dependent on ATP Binding and ATP Hydrolysis

1998 • 584 citations

Heat Shock Proteins Come of Age: Primitive Functions Acquire New Roles in an Adaptive World

1998 • 560 citations

The Amino-terminal Domain of Heat Shock Protein 90 (hsp90) That Binds Geldanamycin Is an ATP/ADP Switch Domain That Regulates hsp90 Conformation

1997 • 554 citations

Targeted Replacement of the Mouse Apolipoprotein E Gene with the Common Human APOE3 Allele Enhances Diet-induced Hypercholesterolemia and Atherosclerosis

1997 • 545 citations

The common 90-kd protein component of non-transformed ‘8S’ steroid receptors is a heat-shock protein.

1985 • 527 citations

Crystal structure of an N-terminal fragment of the DNA gyrase B protein

1991 • 526 citations

Hsp90 chaperones protein folding in vitro

1992 • 514 citations

Steroid receptors and their associated proteins.

1993 • 513 citations

Characterization of a Subset of the Basic-Helix-Loop-Helix-PAS Superfamily That Interacts with Components of the Dioxin Signaling Pathway

1997 • 510 citations

The Heat Shock Protein 90 Antagonist Novobiocin Interacts with a Previously Unrecognized ATP-binding Domain in the Carboxyl Terminus of the Chaperone

2000 • 494 citations

Evidence that the 90-kDa phosphoprotein associated with the untransformed L-cell glucocorticoid receptor is a murine heat shock protein.

1985 • 492 citations

Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.

1996 • 475 citations

Structure of CheA, a Signal-Transducing Histidine Kinase

1999 • 471 citations

The ATPase cycle of Hsp90 drives a molecular clamp' via transient dimerization of the N-terminal domains

2000 • 461 citations

Polyubiquitination and Proteasomal Degradation of the p185c-B-2 Receptor Protein-tyrosine Kinase Induced by Geldanamycin

1996 • 460 citations

Disruption of the Raf-1-Hsp90 Molecular Complex Results in Destabilization of Raf-1 and Loss of Raf-1-Ras Association

1995 • 454 citations

Common non-hormone binding component in non-transformed chick oviduct receptors of four steroid hormones

1984 • 442 citations

Mutational Analysis of Hsp90 Function: Interactions with a Steroid Receptor and a Protein Kinase

1995 • 425 citations

A Novel Cytoplasmic Protein That Interacts with the Ah Receptor, Contains Tetratricopeptide Repeat Motifs, and Augments the Transcriptional Response to 2,3,7,8-Tetrachlorodibenzo-p-dioxin

1997 • 414 citations

ARSACS, a spastic ataxia common in northeastern Québec, is caused by mutations in a new gene encoding an 11.5-kb ORF

2000 • 412 citations

Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)-domain co-chaperones

1999 • 411 citations

Ligand-dependent Interaction of the Aryl Hydrocarbon Receptor with a Novel Immunophilin Homolog in Vivo

1997 • 410 citations

Transformation of MutL by ATP Binding and Hydrolysis

1999 • 400 citations

Evidence That the 90-kDa Heat Shock Protein Is Necessary for the Steroid Binding Conformation of the L Cell Glucocorticoid Receptor

1989 • 394 citations

Hepatitis B Virus X-Associated Protein 2 Is a Subunit of the Unliganded Aryl Hydrocarbon Receptor Core Complex and Exhibits Transcriptional Enhancer Activity

1998 • 375 citations

Purification of the major mammalian heat shock proteins.

1982 • 369 citations

The hsp90-related Protein TRAP1 Is a Mitochondrial Protein with Distinct Functional Properties

2000 • 358 citations

Association of a 59-kilodalton immunophilin with the glucocorticoid receptor complex

1992 • 307 citations

A 90,000-dalton binding protein common to both steroid receptors and the Rous sarcoma virus transforming protein, pp60v-src.

1985 • 304 citations

Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system.

1993 • 302 citations

The 43-kilodalton N-terminal fragment of the DNA gyrase B protein hydrolyzes ATP and binds coumarin drugs

1993 • 302 citations

Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila

1994 • 294 citations

Progesterone Receptor Structure and Function Altered by Geldanamycin, an hsp90-Binding Agent

1995 • 291 citations

Dynamics of heat shock protein 90-progesterone receptor binding and the disactivation loop model for steroid receptor complexes.

1993 • 287 citations

Anti-androgens and the mutated androgen receptor of LNCaP cells: differential effects on binding affinity, heat-shock protein interaction, and transcription activation

1992 • 284 citations

Targeting of the protein chaperone, HSP90, by the transformation suppressing agent, radicicol

1998 • 272 citations

Identification of a 60-kilodalton stress-related protein, p60, which interacts with hsp90 and hsp70.

1993 • 266 citations

Identification of a Protein with Homology to hsp90 That Binds the Type 1 Tumor Necrosis Factor Receptor

1995 • 265 citations

A 59-kilodalton protein associated with progestin, estrogen, androgen, and glucocorticoid receptors

1986 • 261 citations

p50^cdc37 Acting in Concert with Hsp90 Is Required for Raf-1 Function

1999 • 259 citations

p185erbB2 Binds to GRP94 in Vivo

1996 • 259 citations

A role for Hsp90 in cell cycle control: Wee1 tyrosine kinase activity requires interaction with Hsp90.

1994 • 257 citations

NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ

1998 • 256 citations

Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases.

1993 • 245 citations

The endoplasmic reticulum stress protein GRP94, in addition to BiP, associates with unassembled immunoglobulin chains.

1992 • 243 citations

Isolation of MHC Class I-Restricted Tumor Antigen Peptide and Its Precursors Associated with Heat Shock Proteins hsp70, hsp90, and gp96

1999 • 239 citations

The Assembly of Progesterone Receptor-hsp90 Complexes Using Purified Proteins

1998 • 239 citations

Nucleotides and Two Functional States of hsp90

1997 • 238 citations

A Cyclophilin Function in Hsp90-Dependent Signal Transduction

1996 • 233 citations

A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.

1994 • 231 citations

Steroid receptors and their associated proteins

1993 • 231 citations

Polypeptide release by Hsp90 involves ATP hydrolysis and is enhanced by the co-chaperone p23

2000 • 230 citations

Purification of unactivated progesterone receptor and identification of novel receptor-associated proteins.

1990 • 229 citations

A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

1997 • 229 citations

Specific Binding of Tetratricopeptide Repeat Proteins to the C-terminal 12-kDa Domain of hsp90

1998 • 228 citations

Binding of p23 and hsp90 during assembly with the progesterone receptor.

1995 • 224 citations

The Importance of ATP Binding and Hydrolysis by Hsp90 in Formation and Function of Protein Heterocomplexes

1999 • 224 citations

Interactions of p60, a mediator of progesterone receptor assembly, with heat shock proteins hsp90 and hsp70.

1996 • 212 citations

The specific DNA binding activity of the dioxin receptor is modulated by the 90 kd heat shock protein.

1990 • 204 citations

The hsp90-binding Antibiotic Geldanamycin Decreases Raf Levels and Epidermal Growth Factor Signaling without Disrupting Formation of Signaling Complexes or Reducing the Specific Enzymatic Activity of Raf Kinase

1997 • 202 citations

Gene expression profiling of human colon cancer cells following inhibition of signal transduction by 17-allylamino-17-demethoxygeldanamycin, an inhibitor of the hsp90 molecular chaperone

2000 • 200 citations

Inhibition of the oncogene product p185erbB-2 in vitro and in vivo by geldanamycin and dihydrogeldanamycin derivatives

1995 • 198 citations

Binding of heat shock proteins to the avian progesterone receptor.

1989 • 192 citations

Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes.

1994 • 190 citations

Differential interactions of p23 and the TPR-containing proteins Hop, Cyp40, FKBP52 and FKBP51 with Hsp90 mutants

1998 • 190 citations

A New Member of the hsp90 Family of Molecular Chaperones Interacts with the Retinoblastoma Protein during Mitosis and after Heat Shock

1996 • 183 citations

Folding of the Glucocorticoid Receptor by the Reconstituted hsp90-based Chaperone Machinery

1997 • 183 citations

Assembly of progesterone receptor with heat shock proteins and receptor activation are ATP mediated events.

1992 • 178 citations

Cyclophilin 40 (CyP-40), Mapping of Its hsp90 Binding Domain and Evidence That FKBP52 Competes with CyP-40 for hsp90 Binding

1996 • 174 citations

Mechanism of Dimer Formation of the 90‐kDa Heat‐Shock Protein

1995 • 174 citations

The Immunophilin-like Protein XAP2 Regulates Ubiquitination and Subcellular Localization of the Dioxin Receptor

2000 • 174 citations

The Cyclosporin A-binding Immunophilin CyP-40 and the FK506-binding Immunophilin hsp56 Bind to a Common Site on hsp90 and Exist in Independent Cytosolic Heterocomplexes with the Untransformed Glucocorticoid Receptor

1995 • 172 citations

A Model of Protein Targeting Mediated by Immunophilins and Other Proteins That Bind to hsp90 via Tetratricopeptide Repeat Domains

1996 • 163 citations

Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor.

1993 • 161 citations

Conformational activation of a basic helix-loop-helix protein (MyoD1) by the C-terminal region of murine HSP90 (HSP84).

1992 • 155 citations

SBA1 Encodes a Yeast Hsp90 Cochaperone That Is Homologous to Vertebrate p23 Proteins

1998 • 154 citations

Structural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. II. Structure of the active site with ADP or ATP bound to wild type and mutant ATPase fragment.

1994 • 152 citations

The 90-kDa heat shock protein is essential for Ah receptor signaling in a yeast expression system.

1994 • 151 citations

HLA-DR associates with specific stress proteins and is retained in the endoplasmic reticulum in invariant chain negative cells.

1992 • 148 citations

Double-stranded DNA induces the phosphorylation of several proteins including the 90 000 mol. wt. heat-shock protein in animal cell extracts.

1985 • 143 citations

Specific inhibition of cytoplasmic protein tyrosine kinases by herbimycin A in vitro

1991 • 139 citations

erbB-2 oncogene inhibition by geldanamycin derivatives: synthesis, mechanism of action, and structure-activity relationships

1995 • 138 citations

In vitro evidence that hsp90 contains two independent chaperone sites

1997 • 137 citations

KF25706, a novel oxime derivative of radicicol, exhibits in vivo antitumor activity via selective depletion of Hsp90 binding signaling molecules.

1999 • 137 citations

Physical Interaction of Mammalian CDC37 with CDK4

1996 • 135 citations

Role for Hsp90-Associated Cochaperone p23 in Estrogen Receptor Signal Transduction

1999 • 122 citations

p50 Binds Directly to the Catalytic Domain of Raf as Well as to a Site on hsp90 That Is Topologically Adjacent to the Tetratricopeptide Repeat Binding Site

1998 • 119 citations

C-terminal regions of Hsp90 are important for trapping the nucleotide during the ATPase cycle 1 1Edited by R. Huber

2000 • 118 citations

Heat Shock Protein 84 Forms a Complex with Mutant p53 Protein Predominantly within a Cytoplasmic Compartment of the Cell

1996 • 117 citations

Hsp90 Is Required for Pheromone Signaling in Yeast

1998 • 116 citations

Depletion of the erbB-2 gene product p185 by benzoquinoid ansamycins.

1994 • 115 citations

Interaction of Endoplasmic Reticulum Chaperone GRP94 with Peptide Substrates Is Adenine Nucleotide-independent

1997 • 115 citations

Cdc37: a protein kinase chaperone?

1997 • 113 citations

Subunit composition of the molybdate-stabilized “8-9 S” nontransformed estradiol receptor purified from calf uterus.

1987 • 112 citations

The Common Tetratricopeptide Repeat Acceptor Site for Steroid Receptor-associated Immunophilins and Hop Is Located in the Dimerization Domain of Hsp90

1999 • 112 citations

Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein.

1996 • 108 citations

Two FKBP-related proteins are associated with progesterone receptor complexes.

1993 • 106 citations

Genetic and Biochemical Analysis of p23 and Ansamycin Antibiotics in the Function of Hsp90-Dependent Signaling Proteins

1998 • 105 citations

Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation.

1994 • 104 citations

The Hsp Organizer Protein Hop Enhances the Rate of but Is Not Essential for Glucocorticoid Receptor Folding by the Multiprotein Hsp90-based Chaperone System

2000 • 103 citations

Monovalent cation selectivity for ATP-dependent association of the glucocorticoid receptor with hsp70 and hsp90.

1992 • 101 citations

Assessment of the ATP Binding Properties of Hsp90

1996 • 98 citations

Role of Escherichia coli heat shock proteins DnaK and HtpG (C62.5) in response to nutritional deprivation

1990 • 97 citations

Possible Role for Serine/Threonine Phosphorylation in the Regulation of the Heteroprotein Complex between the hsp90 Stress Protein and the pp60v-src Tyrosine Kinase

1995 • 97 citations

Dynamics of heat shock protein 90-progesterone receptor binding and the disactivation loop model for steroid receptor complexes

1993 • 95 citations

Mineralocorticosteroid Receptor of the chick intestine

1989 • 94 citations

Radicicol Leads to Selective Depletion of Raf Kinase and Disrupts K-Ras-activated Aberrant Signaling Pathway

1998 • 94 citations

CNS1 Encodes an Essential p60/Sti1 Homolog in Saccharomyces cerevisiae That Suppresses Cyclophilin 40 Mutations and Interacts with Hsp90

1998 • 90 citations

Structural and functional aspects of basic helix-loop-helix protein folding by heat-shock protein 90.

1994 • 88 citations

Hsp90 Is Essential for the Synthesis and Subsequent Membrane Association, But Not the Maintenance, of the Src-Kinase p56^lck

2000 • 87 citations

Studies with Purified Chaperones Advance the Understanding of the Mechanism of Glucocorticoid Receptor–hsp90 Heterocomplex Assembly

1998 • 87 citations

The dioxin and peroxisome proliferator-activated receptors: nuclear receptors in search of endogenous ligands

1992 • 85 citations

Purification of a Novel Calcium-independent Phospholipase A2 from Rabbit Kidney

1996 • 84 citations

Cns1 Is an Essential Protein Associated with the Hsp90 Chaperone Complex in Saccharomyces cerevisiae That Can Restore Cyclophilin 40-Dependent Functions in cpr7ΔCells

1998 • 84 citations

Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth

1996 • 81 citations

Nucleotide Binding to the 43-Kilodalton N-Terminal Fragment of the DNA Gyrase B Protein

1995 • 80 citations

Binding of Immunophilins to the 90 kDa Heat Shock Protein (hsp90) via a Tetratricopeptide Repeat Domain Is a Conserved Protein Interaction in Plants

1996 • 79 citations

The Basic Helix-Loop-Helix/PAS Factor Sim Is Associated with hsp90:

1995 • 76 citations

Overlapping Sites of Tetratricopeptide Repeat Protein Binding and Chaperone Activity in Heat Shock Protein 90

2000 • 75 citations

Monomer Arrangement in HSP90 Dimer as Determined by Decoration with N and C-Terminal Region Specific Antibodies

1999 • 72 citations

Association of Hsp47, Grp78, and Grp94 with procollagen supports the successive or coupled action of molecular chaperones

1994 • 72 citations

Hsp90 Binds and Regulates the Ligand-Inducible α Subunit of Eukaryotic Translation Initiation Factor Kinase Gcn2

1999 • 72 citations

FKBP54, a novel FK506-binding protein in avian progesterone receptor complexes and HeLa extracts.

1993 • 72 citations

Binding of p23 and hsp90 during assembly with the progesterone receptor

1995 • 67 citations

Cyclophilin-40: evidence for a dimeric complex with hsp90

1995 • 65 citations

Mutational analysis of hsp90 binding to the progesterone receptor.

1993 • 65 citations

The Sch9 protein kinase regulates Hsp90 chaperone complex signal transduction activity in vivo

1999 • 62 citations

Structural Transitions Accompanying the Activation of Peptide Binding to the Endoplasmic Reticulum Hsp90 Chaperone GRP94

1998 • 61 citations

The Yeast CDC37 Gene Interacts with MPS1 and Is Required for Proper Execution of Spindle Pole Body Duplication

1997 • 59 citations

Cdc37 Promotes the Stability of Protein Kinases Cdc28 and Cak1

2000 • 56 citations

Binding of hsp90 to the Glucocorticoid Receptor Requires a Specific 7-Amino Acid Sequence at the Amino Terminus of the Hormone-binding Domain

1998 • 54 citations

A mutation in the ectodomain of herpes simplex virus 1 glycoprotein B causes defective processing and retention in the endoplasmic reticulum

1991 • 52 citations

The molecular chaperone Cdc37 is required for Ste11 function and pheromone‐induced cell cycle arrest

2000 • 52 citations

Modular Folding and Evidence for Phosphorylation-induced Stabilization of an hsp90-dependent Kinase

1998 • 51 citations

Genetic interactions between Hsp90 and the Cdc2 mitotic machineryin the fission yeast Schizosaccharomyces pombe

1999 • 50 citations

Interactions of p60, a mediator of progesterone receptor assembly, with heat shock proteins hsp90 and hsp70

1996 • 49 citations

Binding of Benzoquinoid Ansamycins to p100 Correlates with Their Ability to Deplete the erbB2 Gene Product p185

1994 • 48 citations

Chaperones in progesterone receptor complexes

2000 • 48 citations

The Seven Amino Acids (547–553) of Rat Glucocorticoid Receptor Required for Steroid and Hsp90 Binding Contain a Functionally Independent LXXLL Motif That Is Critical for Steroid Binding

1999 • 47 citations

Labeling of v‐Src and BCR‐ABL tyrosine kinases with [¹⁴C]herbimycin A and its use in the elucidation of the kinase inactivation mechanism

1994 • 44 citations

Use of the Thiol-specific Derivatizing Agent N-Iodoacetyl-3- I iodotyrosine to Demonstrate Conformational Differences between the Unbound and hsp90-bound Glucocorticoid Receptor Hormone Binding Domain

1996 • 44 citations

83‐Kilodalton heat shock proteins of trypanosomes are potent peptide‐stimulated ATPases

1992 • 44 citations

Isolation of untransformed bovine estrogen receptor without molybdate stabilization

1990 • 41 citations

Molecular Mechanisms of Peptide Loading by the Tumor Rejection Antigen/Heat Shock Chaperone gp96 (GRP94)

1999 • 40 citations

The role of the aryl hydrocarbon receptor nuclear translocator protein in aryl hydrocarbon receptor action

1994 • 34 citations

A subpopulation of the v-erb A oncogene protein, a derivative of a thyroid hormone receptor, associates with heat shock protein 90

1991 • 29 citations

Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein

1996 • 26 citations

Inhibition of Ret Tyrosine Kinase Activity by Herbimycin A

1993 • 17 citations