In Vivo Function of Hsp90 Is Dependent on ATP Binding and ATP Hydrolysis

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

THE HEAT-SHOCK PROTEINS

1988 • 5,214 citations

Protein folding in the cell

1992 • 4,138 citations

Molecular chaperones in cellular protein folding

1996 • 3,540 citations

Raster3D Version 2.0. A program for photorealistic molecular graphics

1994 • 2,515 citations

Steroid Receptor Interactions with Heat Shock Protein and Immunophilin Chaperones*

1997 • 1,678 citations

Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for stress proteins in oncogenic transformation.

1994 • 1,426 citations

Crystal Structure of an Hsp90–Geldanamycin Complex: Targeting of a Protein Chaperone by an Antitumor Agent

1997 • 1,345 citations

Identification and Structural Characterization of the ATP/ADP-Binding Site in the Hsp90 Molecular Chaperone

1997 • 1,235 citations

hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.

1989 • 624 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

The Amino-terminal Domain of Heat Shock Protein 90 (hsp90) That Binds Geldanamycin Is an ATP/ADP Switch Domain That Regulates hsp90 Conformation

1997 • 554 citations

Crystal structure of an N-terminal fragment of the DNA gyrase B protein

1991 • 526 citations

Hsp90 chaperones protein folding in vitro

1992 • 514 citations

Mutational Analysis of Hsp90 Function: Interactions with a Steroid Receptor and a Protein Kinase

1995 • 425 citations

The human cytosolic molecular chaperones hsp90, hsp70 (hsc70) and hdj-1 have distinct roles in recognition of a non-native protein and protein refolding.

1996 • 424 citations

Supervising the fold: functional principles of molecular chaperones

1996 • 420 citations

Using the two-hybrid system to detect protein-protein interactions

1993 • 414 citations

Pharmacologic shifting of a balance between protein refolding and degradation mediated by Hsp90

1996 • 406 citations

Chaperone Function of Hsp90-Associated Proteins

1996 • 376 citations

In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone

1997 • 367 citations

Transient Interaction of Hsp90 with Early Unfolding Intermediates of Citrate Synthase

1995 • 365 citations

Hop Modulates hsp70/hsp90 Interactions in Protein Folding

1998 • 351 citations

Progesterone Receptor Structure and Function Altered by Geldanamycin, an hsp90-Binding Agent

1995 • 291 citations

Chaperones get in touch: the Hip-Hop connection

1997 • 289 citations

Dynamics of heat shock protein 90-progesterone receptor binding and the disactivation loop model for steroid receptor complexes.

1993 • 287 citations

Stable and specific binding of heat shock protein 90 by geldanamycin disrupts glucocorticoid receptor function in intact cells.

1996 • 259 citations

A role for Hsp90 in cell cycle control: Wee1 tyrosine kinase activity requires interaction with Hsp90.

1994 • 257 citations

Two chaperone sites in Hsp90 differing in substrate specificity and ATP dependence

1998 • 244 citations

hsp82 Is an Essential Protein That Is Required in Higher Concentrations for Growth of Cells at Higher Temperatures

1989 • 244 citations

A pathway of multi-chaperone interactions common to diverse regulatory proteins: estrogen receptor, Fes tyrosine kinase, heat shock transcription factor Hsf1, and the aryl hydrocarbon receptor

1996 • 240 citations

Nucleotides and Two Functional States of hsp90

1997 • 238 citations

A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.

1994 • 231 citations

A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

1997 • 229 citations

Specific Binding of Tetratricopeptide Repeat Proteins to the C-terminal 12-kDa Domain of hsp90

1998 • 228 citations

Binding of p23 and hsp90 during assembly with the progesterone receptor.

1995 • 224 citations

Interactions of p60, a mediator of progesterone receptor assembly, with heat shock proteins hsp90 and hsp70.

1996 • 212 citations

The hsp90-binding Antibiotic Geldanamycin Decreases Raf Levels and Epidermal Growth Factor Signaling without Disrupting Formation of Signaling Complexes or Reducing the Specific Enzymatic Activity of Raf Kinase

1997 • 202 citations

Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes.

1994 • 190 citations

Folding of the Glucocorticoid Receptor by the Reconstituted hsp90-based Chaperone Machinery

1997 • 183 citations

Chaperones: Helpers Along the Pathways to Protein Folding

1993 • 174 citations

Mechanism of Dimer Formation of the 90‐kDa Heat‐Shock Protein

1995 • 174 citations

Protein Folding In Vivo: Unraveling Complex Pathways

1997 • 170 citations

Reconstitution of the Steroid Receptor·hsp90 Heterocomplex Assembly System of Rabbit Reticulocyte Lysate

1996 • 160 citations

Cooperative Action of Hsp70, Hsp90, and DnaJ Proteins in Protein Renaturation

1996 • 157 citations

SBA1 Encodes a Yeast Hsp90 Cochaperone That Is Homologous to Vertebrate p23 Proteins

1998 • 154 citations

The 90-kDa heat shock protein (hsp-90) possesses an ATP binding site and autophosphorylating activity.

1991 • 147 citations

In vitro evidence that hsp90 contains two independent chaperone sites

1997 • 137 citations

ATP induces a conformational change of the 90-kDa heat shock protein (hsp90).

1993 • 133 citations

Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.

1984 • 133 citations

ATP-binding Properties of Human Hsp90

1997 • 107 citations

Regulation of the heat shock conjugate Hsc70 in the mammalian cell: the characterization of the anti-apoptotic protein BAG-1 provides novel insights.

1998 • 106 citations

Genetic and Biochemical Analysis of p23 and Ansamycin Antibiotics in the Function of Hsp90-Dependent Signaling Proteins

1998 • 105 citations

Assessment of the ATP Binding Properties of Hsp90

1996 • 98 citations

Identifying the catalytic residue of the ATPase reaction of DNA gyrase.

1993 • 94 citations

Characterization of the Nucleotide Binding Properties and ATPase Activity of Recombinant Hamster BiP Purified from Bacteria

1995 • 89 citations

Endoplasmic Reticulum Chaperone GRP94 Subunit Assembly Is Regulated through a Defined Oligomerization Domain

1996 • 74 citations

Threonine 204 of the chaperone protein Hsc70 influences the structure of the active site, but is not essential for ATP hydrolysis.

1993 • 62 citations

Characterization of a Novel 23-Kilodalton Protein of Unactive Progesterone Receptor Complexes

1994 • 38 citations

Minireview

1998 • 32 citations