The Amino-terminal Domain of Heat Shock Protein 90 (hsp90) That Binds Geldanamycin Is an ATP/ADP Switch Domain That Regulates hsp90 Conformation

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Principles of biochemistry

1978 • 5,242 citations

Steroid Receptor Interactions with Heat Shock Protein and Immunophilin Chaperones*

1997 • 1,678 citations

Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for stress proteins in oncogenic transformation.

1994 • 1,426 citations

Crystal Structure of an Hsp90–Geldanamycin Complex: Targeting of a Protein Chaperone by an Antitumor Agent

1997 • 1,345 citations

Structure of a Peptide Inhibitor Bound to the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase

1991 • 949 citations

Principles of biochemistry

1994 • 930 citations

An atypical topoisomerase II from archaea with implications for meiotic recombination

1997 • 890 citations

Biochemistry and genetics of eukaryotic mismatch repair.

1996 • 598 citations

Crystal structure of an N-terminal fragment of the DNA gyrase B protein

1991 • 526 citations

Preclinical pharmacologic evaluation of geldanamycin as an antitumor agent

1995 • 496 citations

Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.

1996 • 475 citations

Disruption of the Raf-1-Hsp90 Molecular Complex Results in Destabilization of Raf-1 and Loss of Raf-1-Ras Association

1995 • 454 citations

The human cytosolic molecular chaperones hsp90, hsp70 (hsc70) and hdj-1 have distinct roles in recognition of a non-native protein and protein refolding.

1996 • 424 citations

Pharmacologic shifting of a balance between protein refolding and degradation mediated by Hsp90

1996 • 406 citations

Transient Interaction of Hsp90 with Early Unfolding Intermediates of Citrate Synthase

1995 • 365 citations

Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase.

1996 • 322 citations

Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids

1997 • 310 citations

Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90.

1996 • 309 citations

Raf exists in a native heterocomplex with hsp90 and p50 that can be reconstituted in a cell-free system.

1993 • 302 citations

Progesterone Receptor Structure and Function Altered by Geldanamycin, an hsp90-Binding Agent

1995 • 291 citations

Destabilization of Raf-1 by Geldanamycin Leads to Disruption of the Raf-1–MEK–Mitogen-Activated Protein Kinase Signalling Pathway

1996 • 274 citations

The native structure of the activated Raf protein kinase is a membrane-bound multi-subunit complex.

1994 • 261 citations

Stable and specific binding of heat shock protein 90 by geldanamycin disrupts glucocorticoid receptor function in intact cells.

1996 • 259 citations

p185erbB2 Binds to GRP94 in Vivo

1996 • 259 citations

A role for Hsp90 in cell cycle control: Wee1 tyrosine kinase activity requires interaction with Hsp90.

1994 • 257 citations

Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases.

1993 • 245 citations

A pathway of multi-chaperone interactions common to diverse regulatory proteins: estrogen receptor, Fes tyrosine kinase, heat shock transcription factor Hsf1, and the aryl hydrocarbon receptor

1996 • 240 citations

Nucleotides and Two Functional States of hsp90

1997 • 238 citations

Positionally cloned human disease genes: Patterns of evolutionary conservation and functional motifs

1997 • 237 citations

A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.

1994 • 231 citations

A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

1997 • 229 citations

Binding of p23 and hsp90 during assembly with the progesterone receptor.

1995 • 224 citations

Interaction of the Rous Sarcoma Virus Protein pp60 src with the Cellular Proteins pp50 and pp90

1986 • 223 citations

The hsp90-binding Antibiotic Geldanamycin Decreases Raf Levels and Epidermal Growth Factor Signaling without Disrupting Formation of Signaling Complexes or Reducing the Specific Enzymatic Activity of Raf Kinase

1997 • 202 citations

Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes.

1994 • 190 citations

How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the insulin receptor protein‐tyrosine kinase

1995 • 187 citations

Geldanamycin selectively destabilizes and conformationally alters mutated p53.

1995 • 185 citations

Cooperative Action of Hsp70, Hsp90, and DnaJ Proteins in Protein Renaturation

1996 • 157 citations

The 90-kDa heat shock protein (hsp-90) possesses an ATP binding site and autophosphorylating activity.

1991 • 147 citations

ATP induces a conformational change of the 90-kDa heat shock protein (hsp90).

1993 • 133 citations

The 23-kDa Acidic Protein in Reticulocyte Lysate Is the Weakly Bound Component of the hsp Foldosome That Is Required for Assembly of the Glucocorticoid Receptor into a Functional Heterocomplex with hsp90

1995 • 122 citations

Interaction of Endoplasmic Reticulum Chaperone GRP94 with Peptide Substrates Is Adenine Nucleotide-independent

1997 • 115 citations

Depletion of p185 erbB2 , Raf-1 and mutant p53 proteins by geldanamycin derivatives correlates with antiproliferative activity

1997 • 104 citations

Assessment of the ATP Binding Properties of Hsp90

1996 • 98 citations

Association of Hsp90 with Cellular Src-Family Kinases in a Cell-Free System Correlates with Altered Kinase Structure and Function

1994 • 93 citations

Nerve Growth Factor-mediated Activation of the Mitogen-activated Protein (MAP) Kinase Cascade Involves a Signaling Complex Containing B-Raf and HSP90

1996 • 88 citations

A role for Hsp90 in retinoid receptor signal transduction.

1995 • 82 citations

Effect of Geldanamycin on the Kinetics of Chaperone-Mediated Renaturation of Firefly Luciferase in Rabbit Reticulocyte Lysate

1996 • 77 citations

The Basic Helix-Loop-Helix/PAS Factor Sim Is Associated with hsp90:

1995 • 76 citations

γ‐Phosphate‐linked ATP‐Sepharose for the affinity purification of protein kinases

1993 • 76 citations

Hsp90-Mediated Folding of the Lymphoid Cell Kinase p56^lck

1996 • 66 citations

Mutational analysis of hsp90 binding to the progesterone receptor.

1993 • 65 citations

The steroid binding domain influences intracellular solubility of the baculovirus overexpressed glucocorticoid and mineralocorticoid receptors

1993 • 48 citations

A predicted consensus structure for the N-Terminal fragment of the heat shock protein HSP90 family

1997 • 26 citations

Characterization of the Hydrophobic Region of Heat Shock Protein 90

1991 • 23 citations

Cell cycle‐dependent association of Gag‐Mil and hsp90

1994 • 19 citations

Preparation of 17‐amino‐22‐(4′‐azido‐3′‐¹²⁵iodophenacyl)‐17‐demethoxygeldanamycin (1): An ansamycin for photoaffinity labeling

1994 • 10 citations