Heterologous expression systems for P-glycoprotein:E. coli, yeast, and baculovirus

ABC Transporters: From Microorganisms to Man

1992 • 3,956 citations

BIOCHEMISTRY OF MULTIDRUG RESISTANCE MEDIATED BY THE MULTIDRUG TRANSPORTER

1993 • 3,515 citations

A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants

1976 • 3,228 citations

Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells

1986 • 1,903 citations

Mammalian multidrug resistance gene: Complete cDNA sequence indicates strong homology to bacterial transport proteins

1986 • 1,058 citations

Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR)

1992 • 937 citations

The multidrug transporter, a double-edged sword.

1988 • 763 citations

Human P-glycoprotein transports cyclosporin A and FK506.

1993 • 676 citations

Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase.

1992 • 629 citations

Phosphatidylcholine translocase: A physiological role for the mdr2 gene

1994 • 600 citations

Volume-regulated chloride channels associated with the human multidrug-resistance P-glycoprotein

1992 • 567 citations

Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli.

1991 • 547 citations

Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance

1991 • 508 citations

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

1991 • 464 citations

An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene

1988 • 430 citations

Partial purification and reconstitution of the human multidrug-resistance pump: characterization of the drug-stimulatable ATP hydrolysis.

1992 • 395 citations

A method for producing recombinant baculovirus expression vectors at high frequency.

1993 • 348 citations

Separation of drug transport and chloride channel functions of the human multidrug resistance P-glycoprotein

1992 • 328 citations

Characterization of the adenosine triphosphatase activity of Chinese hamster P-glycoprotein.

1993 • 220 citations

Abundant bacterial expression and reconstitution of an intrinsic membrane-transport protein from bovine mitochondria

1993 • 219 citations

Functional Complementation of Yeast ste6 by a Mammalian Multidrug Resistance mdr Gene

1992 • 218 citations

Functional consequences of proline mutations in the predicted transmembrane domain of P-glycoprotein.

1993 • 218 citations

Characterization of the azidopine and vinblastine binding site of P-glycoprotein.

1992 • 217 citations

N-glycosylation and deletion mutants of the human MDR1 P-glycoprotein.

1993 • 209 citations

Two different regions of Phosphoglycoprotein are photoaffinity-labeled by azidopine

1989 • 196 citations

Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within, or immediately C-terminal to, transmembrane domains 6 and 12

1993 • 194 citations

A single amino acid substitution strongly modulates the activity and substrate specificity of the mouse mdr1 and mdr3 drug efflux pumps.

1991 • 187 citations

The cystic fibrosis mutation (ΔF508) does not influence the chloride channel activity of CFTR

1993 • 187 citations

Reconstitution of drug-stimulated ATPase activity following co-expression of each half of human P-glycoprotein as separate polypeptides.

1994 • 174 citations

Expression of the yeast plasma membrane [H+]ATPase in secretory vesicles. A new strategy for directed mutagenesis.

1991 • 152 citations

P-glycoproteins: mediators of multidrug resistance

1993 • 143 citations

Amino acid substitutions in the sixth transmembrane domain of P-glycoprotein alter multidrug resistance.

1992 • 140 citations

Cytoplasmic Orientation and Two-domain Structure of the Multidrug Transporter, P-glycoprotein, Demonstrated with Sequence-specific Antibodies

1989 • 135 citations

Functional expression of P-glycoproteins in secretory vesicles.

1994 • 134 citations

Functional consequences of glycine mutations in the predicted cytoplasmic loops of P-glycoprotein.

1994 • 127 citations

Functional consequences of phenylalanine mutations in the predicted transmembrane domain of P-glycoprotein.

1993 • 126 citations

Stability and covalent modification of P-glycoprotein in multidrug-resistant KB cells

1988 • 125 citations

Expression of the human multidrug transporter in insect cells by a recombinant baculovirus

1990 • 119 citations

Antiestrogens and steroid hormones: Substrates of the human P-glycoprotein

1994 • 115 citations

Identification of residues in the first cytoplasmic loop of P-glycoprotein involved in the function of chimeric human MDR1-MDR2 transporters.

1992 • 114 citations

Multiple or pleiotropic drug resistance in yeast

1991 • 97 citations

Hyperexpression and purification ofEscherichia coliadenylate cyclase using a vector designed for expression of lethal gene products

1989 • 95 citations

Lipophilic cations: a group of model substrates for the multidrug-resistance transporter

1992 • 94 citations

Interaction of bioactive hydrophobic peptides with the human multidrug transporter

1994 • 90 citations

Localization of the forskolin labeling sites to both halves of P-glycoprotein: similarity of the sites labeled by forskolin and prazosin.

1994 • 89 citations

Functional expression of human mdr1 in the yeast Saccharomyces cerevisiae.

1992 • 83 citations

Membrane topology of the N-terminal half of the hamster P-glycoprotein molecule

1993 • 82 citations

Direct demonstration of high affinity interactions of immunosuppressant drugs with the drug binding site of the human P-glycoprotein.

1994 • 80 citations

Overexpression and purification of the carboxyl-terminal nucleotide-binding domain from mouse P-glycoprotein. Strategic location of a tryptophan residue.

1994 • 71 citations

Membrane topology of multidrug resistance protein expressed in Escherichia coli. N-terminal domain.

1994 • 67 citations

Modulation of P-Glycoprotein by Protein Kinase C.alpha. in a Baculovirus Expression System

1994 • 65 citations

Deletion and Insertion Mutants of the Multidrug Transporter

1989 • 63 citations

Functional expression of P-glycoprotein in Saccharomyces cerevisiae confers cellular resistance to the immunosuppressive and antifungal agent FK520.

1994 • 63 citations

Functional expression of mouse mdr1 in Escherichia coli.

1993 • 60 citations

Functional expression of P-glycoprotein encoded by the mouse mdr3 gene in yeast cells.

1993 • 56 citations

Use of recombinant P-glycoprotein fragments to produce antibodies to the multidrug transporter

1990 • 54 citations

Expression of the human erythrocyte glucose transporter in Escherichia coli.

1988 • 52 citations

Transmembrane orientation and topogenesis of the third and fourth membrane-spanning regions of human P-glycoprotein (MDR1).

1994 • 49 citations

Biotechnology of β-adrenergic receptors

1990 • 41 citations

Pseudomonas exotoxin fusion proteins are potent immunogens for raising antibodies against P-glycoprotein.

1991 • 36 citations

Interaction of prenylcysteine methyl esters with the multidrug resistance transporter.

1994 • 32 citations

Overexpression of higher eukaryotic membrane proteins in bacteria. Novel insights obtained with the liver mitochondrial proton/phosphate symporter.

1992 • 24 citations

Overexpression, characterization, and purification of a recombinant mouse immunophilin FKBP-52 and identification of an associated phosphoprotein.

1993 • 23 citations

Dedicated Transporters for Peptide Export and Intercompartmental Traffic in the Yeast Saccharomyces cerevisiae

1992 • 22 citations

Expression of Human P-Glycoprotein in Yeast Cells. Effects of Membrane Component Sterols on the Activity of P-Glycoprotein.

1991 • 20 citations

Functional expression of P-glycoprotein in Saccharomyces cerevisiae confers cellular resistance to the immunosuppressive and antifungal agent FK520

1994 • 11 citations