Expression of the yeast plasma membrane [H+]ATPase in secretory vesicles. A new strategy for directed mutagenesis.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

THE COLORIMETRIC DETERMINATION OF PHOSPHORUS

1925 • 19,115 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid Proteins

1981 • 2,972 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range

1972 • 2,632 citations

Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.

1988 • 1,737 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA

1985 • 849 citations

Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases

1986 • 801 citations

Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

1981 • 542 citations

Structure and function of proton translocating ATPase in plasma membranes of plants and fungi

1988 • 464 citations

[76] β-d-Fructofuranoside fructohydrolase from yeast

1975 • 455 citations

In vivo glucose activation of the yeast plasma membrane ATPase

1983 • 391 citations

The effects of vanadate on the plasma membrane ATPase of Neurospora crassa.

1979 • 231 citations

Purification and characterization of constitutive secretory vesicles from yeast.

1987 • 179 citations

Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae.

1987 • 167 citations

Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.

1986 • 166 citations

Solubilization by lysolecithin and purification of the plasma membrane ATPase of the yeast Schizosaccharomyces pombe.

1978 • 157 citations

Current-voltage relationships for the plasma membrane and its principal electrogenic pump inNeurospora crassa: I. Steady-state conditions

1978 • 156 citations

Purification and characterization of the plasma membrane ATPase of Neurospora crassa.

1981 • 138 citations

Replacement of the promoter of the yeast plasma membrane ATPase gene by a galactose-dependent promoter and its physiological consequences

1987 • 131 citations

Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae.

1987 • 128 citations

A second transport ATPase gene in Saccharomyces cerevisiae.

1988 • 125 citations

Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase.

1986 • 124 citations

Membrane potential defect in hygromycin B-resistant pma1 mutants of Saccharomyces cerevisiae.

1988 • 119 citations

Expression of calf prochymosin in Saccharomyces cerevisiae

1984 • 115 citations

Dissection of functional domains of the yeast proton-pumping ATPase by directed mutagenesis.

1988 • 112 citations

Mutation of a conserved glycine residue modifies the vanadate sensitivity of the plasma membrane H+-ATPase from Schizosaccharomyces pombe.

1987 • 108 citations

Growth control strength and active site of yeast plasma membrane ATPase studied by site‐directed mutagenesis

1989 • 108 citations

Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme.

1984 • 97 citations

Defective H+-ATPase of Hygromycin B-resistant pma1 Mutants from Saccharomyces cerevisiae

1989 • 93 citations

Identification of the phosphorylated intermediate of the Neurospora plasma membrane H+-ATPase as beta-aspartyl phosphate.

1981 • 89 citations

Biosynthesis of acid phosphatase of baker's yeast. Factors influencing its production by protoplasts and characterization of the secreted enzyme

1972 • 84 citations

Characterization of the beta-aspartyl phosphate intermediate formed by the H+-translocating ATPase from the yeast Schizosaccharomyces pombe.

1982 • 76 citations

Secretory vesicles externalize the major plasma membrane ATPase in yeast.

1988 • 68 citations

The Amino and Carboxyl Termini of the Neurospora Plasma Membrane H+-ATPase Are Cytoplasmically Located

1989 • 63 citations

Coincident localization of secretory and plasma membrane proteins in organelles of the yeast secretory pathway

1988 • 56 citations

Direct evidence for the cytoplasmic location of the NH2- and COOH-terminal ends of the Neurospora crassa plasma membrane H+-ATPase.

1990 • 51 citations

Mutations of the yeast plasma membrane H+-ATPase which cause thermosensitivity and altered regulation of the enzyme.

1988 • 49 citations

Molecular properties of the fungal plasma-membrane [H+]-ATPase

1989 • 49 citations

Large-scale isolation of the Neurospora plasma membrane H+-ATPase

1984 • 44 citations

A temperature‐sensitive mutant of the yeast plasma membrane ATPase obtained by in vitro mutagenesis

1986 • 20 citations