The Use of Monoclonal Antibodies and Limited Proteolysis in Elucidation of Structure—Function Relationships in Proteins

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

Principles that Govern the Folding of Protein Chains

1973 • 6,771 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Prediction of protein antigenic determinants from amino acid sequences.

1981 • 3,715 citations

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.

1985 • 2,450 citations

The relation between the divergence of sequence and structure in proteins.

1986 • 2,373 citations

Why genes in pieces?

1978 • 2,256 citations

Allosteric proteins and cellular control systems

1963 • 2,187 citations

Amino Acid Preferences for Specific Locations at the Ends of α Helices

1988 • 1,356 citations

Structural patterns in globular proteins

1976 • 1,286 citations

Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint

1986 • 1,172 citations

Conserved Folding in Retroviral Proteases: Crystal Structure of Synthetic HIV-1 Protease

1989 • 1,155 citations

New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and x-ray-derived accessible sites

1986 • 1,150 citations

Prediction of chain flexibility in proteins

1985 • 1,123 citations

COMPLEXES OF SEQUENTIAL METABOLIC ENZYMES

1987 • 1,019 citations

The Antigenic Structure of Proteins: A Reappraisal

1984 • 957 citations

Comparison of super-secondary structures in proteins

1973 • 885 citations

Nucleation, Rapid Folding, and Globular Intrachain Regions in Proteins

1973 • 826 citations

Structural characterization of folding intermediates in cytochrome c by H-exchange labelling and proton NMR

1988 • 823 citations

Fatty acid synthase, a proficient multifunctional enzyme

1989 • 801 citations

Zinc fingers: Gilt by association

1988 • 799 citations

DYNAMICS OF PROTEINS: ELEMENTS AND FUNCTION

1983 • 681 citations

The DNA Binding Domain of the Rat Liver Nuclear Protein C/EBP Is Bipartite

1989 • 661 citations

Helix Signals in Proteins

1988 • 648 citations

PRINCIPLES THAT DETERMINE THE STRUCTURE OF PROTEINS

1984 • 645 citations

Three-Dimensional Solution Structure of a Single Zinc Finger DNA-Binding Domain

1989 • 630 citations

The helix-turn-helix DNA binding motif

1989 • 616 citations

Correlation between segmental mobility and the location of antigenic determinants in proteins

1984 • 590 citations

NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A

1988 • 583 citations

Continuous and discontinuous protein antigenic determinants

1986 • 582 citations

Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on western blots by monoclonal antibodies

1986 • 539 citations

The role of the α-helix dipole in protein function and structure

1985 • 517 citations

Intrinsic and Extrinsic Factors in Protein Antigenic Structure

1985 • 481 citations

Conformation of peptide fragments of proteins in aqueous solution: implications for initiation of protein folding

1988 • 463 citations

Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme

1979 • 452 citations

Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.

1981 • 412 citations

Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes

1983 • 392 citations

How good are predictions of protein secondary structure?

1983 • 375 citations

Proteins : structures and molecular principles

1983 • 357 citations

Receptor and Antibody Epitopes in Human Growth Hormone Identified by Homolog-Scanning Mutagenesis

1989 • 345 citations

Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes.

1988 • 323 citations

Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins

1989 • 306 citations

Antigenic determinants in proteins coincide with surface regions accessible to large probes (antibody domains).

1986 • 302 citations

Correlation between sites of limited proteolysis and segmental mobility in thermolysin

1986 • 297 citations

Efficient mapping of protein antigenic determinants.

1986 • 292 citations

Do genes-in-pieces imply proteins-in-pieces?

1978 • 287 citations

Location of ‘continuous’ antigenic determinants in the protruding regions of proteins.

1986 • 285 citations

The structure of the flavoenzyme glutathione reductase

1978 • 279 citations

cAMP-dependent Protein Kinase

1989 • 274 citations

Splice Junctions: Association with Variation in Protein Structure

1983 • 261 citations

Thermodynamic Problems of Protein Structure

1989 • 260 citations

Fluctuations in Protein Structure from X-Ray Diffraction

1984 • 254 citations

Microenvironments and Metabolic Compartmentation

1979 • 236 citations

Hierarchic organization of domains in globular proteins

1979 • 228 citations

A simple model for proteins with interacting domains. Applications to scanning calorimetry data

1989 • 227 citations

Folding of Protein Fragments

1981 • 215 citations

Functional domain structure of calcineurin A: mapping by limited proteolysis

1989 • 201 citations

Antibody-antigen complexes.

1988 • 189 citations

TWO-DIMENSIONAL NMR AND PROTEIN STRUCTURE

1989 • 183 citations

Transmembrane topography of the nicotinic acetylcholine receptor: immunochemical tests contadict theoretical predictions based on hydrophobicity profiles

1986 • 183 citations

The Regulation of Carbohydrate Metabolism

1975 • 181 citations

Full-length cDNA for rabbit tryptophan hydroxylase: functional domains and evolution of aromatic amino acid hydroxylases.

1987 • 180 citations

Modular arrangement of functional domains along the sequence of an aminoacyl tRNA synthetase

1983 • 179 citations

Location and Chemical Synthesis of a Binding Site for HIV-1 on the CD4 Protein

1988 • 178 citations

Domain structure and phosphorylation of protein kinase C.

1987 • 168 citations

Carbohydrate binding protein 35. Complementary DNA sequence reveals homology with proteins of the heterogeneous nuclear RNP.

1988 • 160 citations

Monoclonal antibodies as probes of acetylcholine receptor structure. 2. Binding to native receptor

1981 • 157 citations

Purification and properties of rat brain hexokinase

1972 • 151 citations

Predicted structures of cAMP binding domains of type I and II regulatory subunits of cAMP-dependent protein kinase

1987 • 151 citations

The Organization of Cell Metabolism

1986 • 149 citations

Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.

1988 • 149 citations

Do exons code for structural or functional units in proteins?

1988 • 147 citations

Complete amino acid sequence and predicted membrane topology of phenobarbital-induced cytochrome P-450 (isozyme 2) from rabbit liver microsomes.

1983 • 146 citations

Location of antigenic determinants of primary sequences of the subunits of nicotinic acetylcholine receptor by peptide mapping

1986 • 146 citations

Peptide and protein reviews

1983 • 142 citations

Characterization of antisera to a synthetic carboxyl-terminal peptide of the glucose transporter protein.

1988 • 139 citations

The tree structural organization of proteins

1978 • 136 citations

Recognition of super-secondary structure in proteins

1984 • 133 citations

An intact hydrophobic N-terminal sequence is critical for binding of rat brain hexokinase to mitochondria

1985 • 131 citations

The Physical Basis for Induction of Protein-Reactive Antipeptide Antibodies

1988 • 130 citations

Peptide-specific antibodies as probes of the orientation of the glucose transporter in the human erythrocyte membrane.

1987 • 129 citations

Toward a better understanding of protein folding pathways.

1988 • 127 citations

Reaction of monoclonal antibodies with plasma membrane proteins after binding on nitrocellulose: Renaturation of antigenic sites and reduction of nonspecific antibody binding

1987 • 125 citations

Homology between phenylalanine and tyrosine hydroxylases reveals common structural and functional domains

1985 • 121 citations

Antigenicity and native structure of globular proteins: low frequency of peptide reactive antibodies.

1987 • 115 citations

Monoclonal antibodies as probes of acetylcholine receptor structure. 1. Peptide mapping

1981 • 115 citations

Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes.

1986 • 111 citations

Complete amino acid sequence of rat brain hexokinase, deduced from the cloned cDNA, and proposed structure of a mammalian hexokinase.

1989 • 110 citations

Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.

1985 • 108 citations

Compact units in proteins

1986 • 105 citations

The rudimentary gene of Drosophila melanogaster encodes four enzymic functions

1987 • 105 citations

Microcompartmentation

2018 • 103 citations

Sequence and domain structure of yeast pyruvate carboxylase.

1988 • 101 citations

Protein antigen-monoclonal antibody contact sites investigated by limited proteolysis of monoclonal antibody-bound antigen: protein "footprinting".

1988 • 99 citations

Effects of the phenylalanine-22 .fwdarw. leucine, glutamic acid-49 .fwdarw. methionine, glycine-234 .fwdarw. aspartic acid and glycine-234 .fwdarw. lysine mutations on the folding and stability of the .alpha. subunit of tryptophan synthase from Escherichia coli

1986 • 99 citations

A Critical Evaluation of Methods for Prediction of Protein Secondary Structures

1988 • 94 citations

Conformation-specific monoclonal antibodies to the calcium-induced structure of protein C.

1986 • 92 citations

Isolation and characterization of the discrete N- and C-terminal halves of rat brain hexokinase: Retention of full catalytic activity in the isolated C-terminal half

1989 • 90 citations

A major part of the polypeptide chain of tobacco mosaic virus protein is antigenic

1985 • 90 citations

Isolation and sequence of a cDNA clone which contains the complete coding region of rat phenylalanine hydroxylase. Structural homology with tyrosine hydroxylase, glucocorticoid regulation, and use of alternate polyadenylation sites.

1986 • 89 citations

Epitope Mapping by Chemical Modification of Free and Antibody-Bound Protein Antigen

1987 • 87 citations

An assessment of prediction methods for locating continuous epitopes in proteins

1988 • 83 citations

Synthetic peptides as antigens

1986 • 83 citations

Monoclonal antibodies specific for type 3 protein kinase C recognize distinct domains of protein kinase C and inhibit in vitro functional activity.

1988 • 83 citations

Rat brain hexokinase: The hydrophobie N-terminus of the mitochondrially bound enzyme is inserted in the lipid bilayer

1988 • 83 citations

Evolutionarily Conserved Sequences of Striated Muscle Myosin Heavy Chain Isoforms

1989 • 82 citations

Mapping surface structures of the human insulin receptor with monoclonal antibodies: localization of main immunogenic regions to the receptor kinase domain

1986 • 81 citations

Isolation of a proteolytically derived domain of the insulin receptor containing the major site of cross-linking/binding

1989 • 80 citations

Channeling of extramitochondrial ornithine to matrix ornithine transcarbamylase.

1987 • 80 citations

Crystal Versus Solution Structures of Enzymes: NMR Spectroscopy of a Crystalline Serine Protease

1989 • 80 citations

Seeding protein folding

1986 • 77 citations

Membrane topology of mammalian cytochromes P-450 from liver endoplasmic reticulum

1989 • 75 citations

Topology of outer membrane pore protein PhoE of Escherichia coli. Identification of cell surface-exposed amino acids with the aid of monoclonal antibodies.

1986 • 75 citations

Monoclonal antibodies with differing affinities to the red-absorbing and far-red-absorbing forms of phytochrome

1985 • 72 citations

Structural and functional influence of enzyme-antibody interactions: effects of eight different monoclonal antibodies on the enzymatic activity of Escherichia coli tryptophan synthase

1984 • 71 citations

Quasi‐irreversibility in the unfolding‐refolding transition of phosphoglycerate kinase induced by guanidine hydrochloride

1987 • 70 citations

Organization of a multifunctional protein in pyrimidine biosynthesis. Analyses of active, tryptic fragments.

1981 • 69 citations

A rapid method of epitope mapping

1988 • 69 citations

Method to map antigenic determinants recognized by monoclonal antibodies: localization of a determinant of virus neutralization on the feline leukemia virus envelope protein gp70.

1984 • 69 citations

Topographic analysis of antigenic determinants recognized by monoclonal antibodies to the photoreceptor guanyl nucleotide-binding protein, transducin.

1987 • 68 citations

Conserved Domains in Molybdenum Hydroxylases

1989 • 65 citations

Limited proteolysis of the nitrate reductase from spinach leaves.

1988 • 63 citations

The molecular organization of human alpha 2-macroglobulin. An immunoelectron microscopic study with monoclonal antibodies.

1988 • 57 citations

Partly native epitopes are already present on early intermediates in the folding of tryptophan synthase.

1987 • 57 citations

Rat brain hexokinase: Location of the allosteric regulatory site in a structural domain at the N-terminus of the enzyme

1987 • 56 citations

Monoclonal antibodies as probes of conformational changes in protein-engineered cytochrome c.

1988 • 55 citations

Tubulin structure probed with antibodies to synthetic peptides. Mapping of three major types of limited proteolysis fragments

1988 • 53 citations

Characterization of transducin from bovine retinal rod outer segments. Use of monoclonal antibodies to probe the structure and function of the subunit.

1988 • 51 citations

Mammalian aspartate transcarbamylase (ATCase): sequence of the ATCase domain and interdomain linker in the CAD multifunctional polypeptide and properties of the isolated domain.

1989 • 51 citations

Approach to the direct intramolecular localization of antigenic determinants in Androctonus australis hemocyanin with monoclonal antibodies by molecular immunoelectron microscopy

1985 • 51 citations

Monoclonal antibodies as probes of domain structure of the spectrin alpha subunit.

1982 • 50 citations

A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen

1986 • 50 citations

Role of the Arg¹⁵⁸ residue of the outer membrane PhoE pore protein of Escherichia coli K 12 in bacteriophage TC 45 recognition and in channel characteristics

1985 • 50 citations

An examination of the expected degree of sequence similarity that might arise in proteins that have converged to similar conformational states

1981 • 47 citations

Functional domains of the in situ red cell membrane calcium pump revealed by proteolysis and monoclonal antibodies

1989 • 46 citations

Rat brain hexokinase: Location of the substrate nucleotide binding site in a structural domain at the C-terminus of the enzyme

1986 • 45 citations

Computer prediction of potential immunogenic determinants from protein amino acid sequence

1987 • 45 citations

Characterization of the antigenic sites on the refined 3-A resolution structure of mouse testicular lactate dehydrogenase C4.

1987 • 44 citations

Monoclonal antibodies specific for each of the two toxin binding sites of Torpedo acetylcholine receptor

1987 • 43 citations

Conformation of cytoplasmic segments of acetylcholine receptor alpha- and beta-subunits probed by monoclonal antibodies: sensitivity of the antibody competition approach.

1987 • 42 citations

Reversible independent unfolding of the domains of urokinase monitored by proton NMR

1989 • 42 citations

Binding of nucleoside triphosphates, inorganic phosphate, and other polyanionic ligands to the N-terminal region of rat brain hexokinase: Relationship to regulation of hexokinase activity by antagonistic interactions between glucose 6-phosphate and inorganic phosphate

1990 • 41 citations

Structural mapping of membrane-bound immunoglobulin E-receptor complexes: use of monoclonal anti-IgE antibodies to probe the conformation of receptor-bound IgE

1985 • 41 citations

C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.

1985 • 40 citations

Characterization of a slow folding reaction for the α subunit of tryptophan synthase

1987 • 40 citations

Detection of intermediates in the unfolding transition of phosphoglycerate kinase using limited proteolysis

1989 • 39 citations

Characterization of the catalytic domain of bovine adrenal tyrosine hydroxylase

1988 • 39 citations

The folding and mutual interaction of the domains of yeast 3‐phosphoglycerate kinase

1985 • 38 citations

Evidence from Cassette Mutagenesis for a Structure-Function Motif in a Protein of Unknown Structure

1988 • 38 citations

Conformational effects of ligand binding on the .beta.2 subunit of Escherichia coli tryptophan-synthase analyzed with monoclonal antibodies

1986 • 37 citations

Monoclonal antibodies against rat brain hexokinase. Utilization in epitope mapping studies and establishment of structure-function relationships.

1985 • 37 citations

Topography of integral membrane proteins: hydrophobicity analysis vs. immunolocalization

1988 • 35 citations

Monoclonal antibody studies of creatine kinase. The ART epitope: evidence for an intermediate in protein folding

1989 • 35 citations

Mapping of catalytic domains and phosphorylation sites in the multifunctional pyrimidine‐biosynthetic protein CAD

1988 • 35 citations

The domain structure of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii

1987 • 35 citations

Proteolytic dissection of rat brain hexokinase: Determination of the cleavage pattern during limited digestion with trypsin

1984 • 35 citations

Rat brain hexokinase: Location of the substrate hexose binding site in a structural domain at the C-terminus of the enzyme

1987 • 34 citations

Site-directed chemical modification of horse cytochrome c results in changes in antigenicity due to local and long-range conformational perturbations.

1987 • 34 citations

The domains of rat liver sulfite oxidase. Proteolytic separation and characterization.

1978 • 34 citations

Conformational changes induced by domain assembly within the β₂ subunit of Escherichia coli tryptophan synthase analysed with monoclonal antibodies

1986 • 34 citations

Kinetic characterization of early intermediates in the folding of E. coli tryptophan‐synthase β₂ subunit

1986 • 34 citations

Ligand-induced conformations of rat brain hexokinase: Studies with hexoses, hexose 6-phosphates, and nucleoside triphosphates leading to development of a new model for the enzyme

1978 • 32 citations

Continuous compact protein domains

1987 • 32 citations

Ligand-induced conformational changes of rat brain hexokinase: Their role in determining the substrate specificity for hexoses and inhibitory effectiveness of hexose 6-phosphates

1979 • 31 citations

The use of monoclonal antibodies to fragments of chicken type IV collagen in structural and localization studies.

1983 • 30 citations

Topographical separation of the catalytic sites of asparagine synthetase explored with monoclonal antibodies.

1987 • 30 citations

Monoclonal antibodies against the renal Na+-D-glucose cotransporter. Identification of antigenic polypeptides and demonstration of functional coupling of different Na+-cotransport systems.

1988 • 29 citations

Mapping of functional domains of human high molecular weight and low molecular weight kininogens using murine monoclonal antibodies

1987 • 29 citations

10-Formyltetrahydrofolate synthetase. Evidence for a conformational change in the enzyme upon binding of tetrahydropteroylpolyglutamates.

1987 • 29 citations

Proteolytic dissection as a probe of conformational changes in the human erythrocyte glucose transport protein

1988 • 28 citations

Monoclonal antibodies against rat brain hexokinase. Effects on catalytic function and binding to the outer mitochondrial membrane.

1984 • 27 citations

Modeling the three-dimensional structures of bacterial aminotransferases

1988 • 27 citations

The anatomy of a multifunctional enzyme

1987 • 26 citations

Dynamic conformational changes of 21 S dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion

1989 • 25 citations

Limited tryptic proteolysis of pig kidney 3,4-dihydroxyphenylalanine decarboxylase

1988 • 25 citations

Nature of Tryptic Attack on Cytochrome b5 and Further Evidence for the Two-Domain Structure of the Cytochrome Molecule1

1978 • 24 citations

Monoclonal antibodies as probes of the topological arrangement of the .alpha.-subunits of Escherichia coli RNA polymerase

1989 • 24 citations

Studies on the binding of nucleotides by rat brain hexokinase

1982 • 23 citations

Structure-Function Analysis of Plasma Cholesteryl Ester Transfer Protein by Protease Digestion and Expression of cDNA Fragments in Escherichia coli

1989 • 23 citations

Mapping of biologically relevant sites on human IL-1 beta using monoclonal antibodies.

1988 • 23 citations

Scanning calorimetry of complex biological structures

1984 • 22 citations

Intracellular location of the multidomain protein CAD in mammalian cells

1988 • 22 citations

Limited proteolysis of T-kininogen (thiostatin)

1989 • 20 citations

Topographical characterization of the domain structure of the bovine adrenal atrial natriuretic factor R1 receptor

1989 • 20 citations

Independent refolding of domains in the pancreatic serine proteinases.

1986 • 19 citations

Effect of monoclonal antibodies on the properties of smooth muscle myosin

1989 • 18 citations

Identification of intracellular degradation intermediates of aldolase B by antiserum to the denatured enzyme.

1985 • 17 citations

Recognition of functional regions in primary structures using a set of property patterns

1989 • 17 citations

Microinjection of deoxynucleotides into mouse cells. No evidence that precursors for DNA synthesis are channeled.

1988 • 17 citations

Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides

1988 • 17 citations

A gel electrophoresis method for epitope mapping studies with monoclonal antibodies

1984 • 16 citations

Monoclonal antibody against an epitope on the cytoplasmic aspect of the plasma membrane calcium pump.

1988 • 15 citations

Peptides mimicking the flap of human renin: synthesis, conformation, and antibody recognition

1988 • 14 citations

Monoclonal antibodies to rabbit skeletal muscle phosphorylase kinase. Probes for studies of subunit function.

1985 • 14 citations

Differential scanning calorimetric study of rat brain hexokinase: Domain structure and stability

1990 • 12 citations

Characterization of five epitopes of human renin from a computer model

1988 • 12 citations

rec-A protein-promoted recombination reaction consists of two independent processes, homologous matching and processive unwinding. A study involving an anti-rec-A protein-monoclonal IgG.

1987 • 9 citations

Limited proteolysis of 3‐phosphoglycerate kinase without loss of enzymic activity

1988 • 8 citations

Two-domain structure of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase

1979 • 7 citations

Hexokinase A from mammalian brain: Comparative peptide mapping and immunological studies with monoclonal antibodies

1986 • 7 citations

Interaction with a monoclonal antibody alters the expression of co-operativity by phenylalanine hydroxylase from rat liver

1989 • 4 citations

Location of enzymatic and DNA‐binding domains on E. coli protease La

1989 • 3 citations

DETECTION OF PROTEIN STRUCTURAL FEATURES WITH PROFILE ANALYSIS

1989 • 1 citations