The Biology of heat shock proteins and molecular chaperones.

Protein folding in the cell

1992 • 4,138 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

Homologous plant and bacterial proteins chaperone oligomeric protein assembly

1988 • 1,316 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

INTERMEDIATES IN THE FOLDING REACTIONS OF SMALL PROTEINS

1990 • 1,133 citations

Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria

1989 • 933 citations

Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.

1991 • 835 citations

Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins

1990 • 756 citations

Reconstitution of active dimeric ribulose bisphosphate carboxylase from an unfolded state depends on two chaperonin proteins and Mg-ATP

1989 • 737 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli

1989 • 703 citations

Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis

1989 • 676 citations

IMPORT OF PROTEINS INTO MITOCHONDRIA

1991 • 583 citations

Protein Sorting to Mitochondria: Evolutionary Conservations of Folding and Assembly

1990 • 494 citations

Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen.

1989 • 492 citations

Purification and properties of groE, a host protein involved in bacteriophage assembly

1979 • 426 citations

Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism

1992 • 422 citations

Chaperonin-mediated protein folding: GroES binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity.

1992 • 412 citations

70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.

1988 • 380 citations

Role of an energized inner membrane in mitochondrial protein import. Delta psi drives the movement of presequences.

1991 • 372 citations

Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria.

1982 • 351 citations

Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor

1989 • 335 citations

Interaction of hsp70 with unfolded proteins: effects of temperature and nucleotides on the kinetics of binding.

1991 • 332 citations

Mitochondrial protein import: Nucleoside triphosphates are involved in conferring import-competence to precursors

1987 • 322 citations

Prevention of Protein Denaturation Under Heat Stress by the Chaperonin Hsp60

1992 • 299 citations

Initiation of lambda DNA replication with purified host- and bacteriophage-encoded proteins: the role of the dnaK, dnaJ and grpE heat shock proteins.

1989 • 294 citations

Requirement of a Membrane Potential for the Posttranslational Transfer of Proteins into Mitochondsria

1982 • 292 citations

Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.

1991 • 284 citations

Adenine nucleotide binding sites on beef heart F1-ATPase. Evidence for three exchangeable sites that are distinct from three noncatalytic sites.

1982 • 282 citations

Protein synthesis in chloroplasts IX. Assembly of newly-synthesized large subunits into ribulose bishopshate carboxylase in isolated intact pea chloroplasts

1980 • 278 citations

The ‘molten globule’ state is involved in the translocation of proteins across membranes?

1988 • 275 citations

A dual role for mitochondrial heat shock protein 70 in membrane translocation of preproteins.

1993 • 274 citations

Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly

1979 • 272 citations

Regulation of Hsp70 function by a eukaryotic DnaJ homolog.

1992 • 262 citations

How do polypeptides cross the mitochondrial membranes?

1990 • 246 citations

A precursor protein partly translocated into yeast mitochondria is bound to a 70 kd mitochondrial stress protein.

1990 • 242 citations

SSC1, an Essential Member of the Yeast HSP70 Multigene Family, Encodes a Mitochondrial Protein

1989 • 242 citations

Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space

1992 • 233 citations

Sequential action of mitochondrial chaperones in protein import into the matrix.

1991 • 221 citations

Nucleo‐mitochondrial interactions in yeast mitochondrial biogenesis

1989 • 216 citations

Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sites.

1989 • 212 citations

The Nuclear Membrane

1992 • 206 citations

The mitochondrial chaperonin hsp60 is required for its own assembly

1990 • 205 citations

Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA

1991 • 204 citations

Identification, characterization, and purification of two mammalian stress proteins present in mitochondria, grp 75, a member of the hsp 70 family and hsp 58, a homolog of the bacterial groEL protein

1989 • 199 citations

A polypeptide bound by the chaperonin groEL is localized within a central cavity.

1993 • 177 citations

Polypeptides traverse the mitochondrial envelope in an extended state

1990 • 172 citations

Mitochondrial precursor protein. Effects of 70-kilodalton heat shock protein on polypeptide folding, aggregation, and import competence.

1990 • 170 citations

Molecular architecture of the inner membrane of mitochondria from rat liver: a combined biochemical and stereological study.

1986 • 163 citations

Presequence and mature part of preproteins strongly influence the dependence of mitochondrial protein import on heat shock protein 70 in the matrix.

1993 • 158 citations

A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins.

1993 • 157 citations

The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation

1993 • 150 citations

MAS5, a Yeast Homolog of DnaJ Involved in Mitochondrial Protein Import

1992 • 143 citations

Translocation of proteins into isolated chloroplasts requires cytosolic factors to obtain import competence

1990 • 142 citations

Role of cytochrome c heme lyase in the import of cytochrome c into mitochondria.

1988 • 140 citations

Identification of a mammalian 10-kDa heat shock protein, a mitochondrial chaperonin 10 homologue essential for assisted folding of trimeric ornithine transcarbamoylase in vitro.

1992 • 139 citations

Translocation and insertion of precursor proteins into isolated outer membranes of mitochondria.

1993 • 135 citations

Identification of a groES-like chaperonin in mitochondria that facilitates protein folding.

1990 • 135 citations

Import of cytochrome c heme lyase into mitochondria: a novel pathway into the intermembrane space.

1992 • 133 citations

Protein folding causes an arrest of preprotein translocation into mitochondria in vivo.

1991 • 132 citations

Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.

1988 • 128 citations

A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast

1991 • 117 citations

Folding of dihydrofolate reductase from Escherichia coli

1986 • 116 citations

Purified presequence binding factor (PBF) forms an import-competent complex with a purified mitochondrial precursor protein.

1990 • 113 citations

The requirement of matrix ATP for the import of precursor proteins into the mitochondrial matrix and intermembrane space

1994 • 107 citations

Unique composition of plastid chaperonin-60: α and β polypeptide-encoding genes are highly divergent

1990 • 106 citations

Mitochondrial protein import: Specific recognition and membrane translocation of preproteins

1993 • 102 citations

ATP10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex.

1990 • 100 citations

Molecular cloning of a Chinese hamster mitochondrial protein related to the “chaperonin” family of bacterial and plant proteins

1989 • 99 citations

Precursor proteins in transit through mitochondrial contact sites interact with hsp70 in the matrix

1990 • 98 citations

A Mutation in the Yeast Heat-Shock Factor Gene Causes Temperature-Sensitive Defects in Both Mitochondrial Protein Import and the Cell Cycle

1991 • 94 citations

A Normal Mitochondrial Protein is Selectively Synthesized and Accumulated During Heat Shock in Tetrahymena Thermophila

1987 • 88 citations

Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.

1989 • 87 citations

Immunological identification of yeast SCO1 protein as a component of the inner mitochondrial membrane

1991 • 86 citations

Apocytochrome c: an exceptional mitochondrial precursor protein using an exceptional import pathway

1990 • 84 citations

Protein import into mitochondria: a paradigm for the translocation of polypeptides across membranes

1993 • 84 citations

The cytosolic factor required for import of precursors of mitochondrial proteins into mitochondria.

1988 • 82 citations

Loss of mitochondrial hsp60 function: nonequivalent effects on matrix-targeted and intermembrane-targeted proteins.

1993 • 79 citations

Energy requirements for unfolding and membrane translocation of precursor proteins during import into mitochondria.

1990 • 67 citations

Presequence binding factor-dependent and -independent import of proteins into mitochondria.

1992 • 63 citations

A matrix ATP requirement for presequence translocation across the inner membrane of mitochondria.

1993 • 60 citations

Function of the maize mitochondrial chaperonin hsp60: specific association between hsp60 and newly synthesized F1-ATPase alpha subunits.

1990 • 58 citations

Cloning and characterization of the yeast chaperonin HSP60 gene

1989 • 56 citations

Targeting of a chemically pure preprotein to mitochondria does not require the addition of a cytosolic signal recognition factor.

1992 • 55 citations

Transport of proteins into the various subcompartments of mitochondria

1992 • 55 citations

The alpha regulatory subunit of the mitochondrial F1-ATPase complex is a heat-shock protein. Identification of two highly conserved amino acid sequences among the alpha-subunits and molecular chaperones.

1990 • 49 citations

Roles of molecular chaperones in protein targeting to mitochondria

1993 • 39 citations

Import of precursor proteins into mitochondria: site of polypeptide unfolding.

1990 • 30 citations

Heat shock proteins of barley mitochondria and chloroplasts Identification of organellar hsp 10 and 12: putative chaperonin 10 homologues

1992 • 30 citations

Dissociation of complexes between 70 kDa stress proteins and presecretory proteins is facilitated by a cytosolic factor

1992 • 30 citations

Intramitochondrial Localization of the Main 70-kDa Heat-Shock Cognate Protein in Drosophila Cells

1993 • 19 citations