A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

THE HEAT-SHOCK RESPONSE

1986 • 4,580 citations

Speculations on the functions of the major heat shock and glucose-regulated proteins

1986 • 1,574 citations

An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

1986 • 1,457 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.

1986 • 896 citations

The Heat Shock Respons

1985 • 809 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

Uncoating ATPase is a member of the 70 kilodalton family of stress proteins

1986 • 607 citations

Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous.

1984 • 513 citations

Mechanism of Protein Translocation Across the Endoplasmic Reticulum Membrane

1986 • 509 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

1984 • 462 citations

Hsp70 accelerates the recovery of nucleolar morphology after heat shock.

1984 • 456 citations

An enzyme that removes clathrin coats: purification of an uncoating ATPase.

1984 • 449 citations

A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.

1987 • 418 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides.

1985 • 365 citations

Complex Interactions Among Members of an Essential Subfamily of hsp70 Genes in Saccharomyces Cerevisiae

1987 • 358 citations

In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent post-translational translocation of the prepro-α-factor

1986 • 332 citations

Involvement of ATP in the nuclear and nucleolar functions of the 70 kd heat shock protein.

1985 • 327 citations

Mitochondrial protein import: Nucleoside triphosphates are involved in conferring import-competence to precursors

1987 • 322 citations

Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.

1986 • 315 citations

The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

1985 • 301 citations

Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth

1984 • 278 citations

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.

1986 • 258 citations

Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form.

1987 • 257 citations

Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.

1987 • 225 citations

Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.

1987 • 206 citations

SSC1, a member of the 70-kDa heat shock protein multigene family of Saccharomyces cerevisiae, is essential for growth.

1987 • 205 citations

Uncoupling Translocation from Translation: Implications for Transport of Proteins Across Membranes

1986 • 195 citations

Secretion in yeast: translocation and glycosylation of prepro-α-factor in vitro can occur via an ATP-dependent post-translational mechanism

1986 • 185 citations

Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.

1982 • 177 citations

Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix

1987 • 175 citations

Constitutively Expressed Rat mRNA Encoding a 70-Kilodalton Heat-Shock-Like Protein

1985 • 164 citations

A purified precursor polypeptide requires a cytosolic protein fraction for import into mitochondria.

1984 • 126 citations

Cell biology: An unfolding story of protein translocation

1986 • 126 citations

Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.

1987 • 119 citations

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

1985 • 107 citations

Constitutively expressed rat mRNA encoding a 70-kilodalton heat-shock-like protein.

1985 • 104 citations

Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor.

1986 • 80 citations

Post-translational insertion of fragment of the glucose transporter into microsomes requires phosphoanhydride bond cleavage

1986 • 75 citations

A human gene family with sequence homology to Drosophila melanogaster Hsp70 heat shock genes.

1986 • 73 citations

The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

1987 • 73 citations

Secretion in yeast: structural features influencing the post-translational translocation of prepro-alpha-factor in vitro.

1987 • 72 citations

Import of honeybee prepromelittin into the endoplasmic reticulum. Requirements for membrane insertion, processing, and sequestration.

1986 • 71 citations

Sequences distal to the mitochondrial targeting sequences are necessary for the maturation of the F1-ATPase beta-subunit precursor in mitochondria.

1987 • 66 citations

The role of protein structure in the mitochondrial import pathway. Analysis of the soluble F1-ATPase beta-subunit precursor.

1987 • 37 citations