Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

Transport of proteins into mitochondria: Translocational intermediates spanning contact sites between outer and inner membranes

1985 • 470 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH.

1982 • 219 citations

Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.

1987 • 206 citations

Transport of F₁‐ATPase subunit β into mitochondria depends on both a membrane potential and nucleoside triphosphates

1986 • 182 citations

Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

1988 • 182 citations

Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix

1987 • 175 citations

Import of an incompletely folded precursor protein into isolated mitochondria requires an energized inner membrane, but no added ATP.

1987 • 119 citations

A cytosolic protein contains a cryptic mitochondrial targeting signal

1987 • 101 citations

Protein translocation: A common mechanism for different membrane systems?

1986 • 31 citations