A Nuclear Export Signal in Kap95p Is Required for Both Recycling the Import Factor and Interaction with the Nucleoporin GLFG Repeat Regions of Nup116p and Nup100p

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Identification of a signal for rapid export of proteins from the nucleus

1995 • 1,186 citations

The HIV-1 Rev Activation Domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs

1995 • 1,127 citations

Nucleocytoplasmic Transport

1996 • 1,071 citations

Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.

1990 • 909 citations

Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm

1992 • 825 citations

Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins

1995 • 767 citations

The GTP-binding protein Ran/TC4 is required for protein import into the nucleus

1993 • 760 citations

Isolation of a protein that is essential for the first step of nuclear protein import

1994 • 710 citations

Identification of different roles for RanGDP and RanGTP in nuclear protein import.

1996 • 644 citations

Identification and characterization of a nuclear pore complex protein

1986 • 610 citations

Nuclear protein migration involves two steps: Rapid binding at the nuclear envelope followed by slower translocation through nuclear pores

1988 • 551 citations

Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor [published erratum appears in J Cell Biol 1994 Jan;124(1-2):217]

1993 • 544 citations

Nuclear import can be separated into distinct steps in vitro: Nuclear pore binding and translocation

1988 • 535 citations

A nuclear export signal in hnRNP A1: A signal-mediated, temperature-dependent nuclear protein export pathway

1995 • 529 citations

Regulation of the Yeast HO Gene

1985 • 526 citations

Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope

1995 • 511 citations

Distinct functions for the two importin subunits in nuclear protein import

1995 • 479 citations

The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex

1995 • 460 citations

Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.

1995 • 430 citations

Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import

1991 • 391 citations

Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62.

1995 • 385 citations

Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences

1995 • 337 citations

Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins

1995 • 308 citations

Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope.

1994 • 306 citations

Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins

1996 • 306 citations

The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm.

1994 • 305 citations

In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.

1995 • 288 citations

Isolation of the yeast nuclear pore complex.

1993 • 287 citations

The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors

1992 • 278 citations

Sequence and characterization of cytoplasmic nuclear protein import factor p97.

1995 • 274 citations

Components of the yeast spindle and spindle pole body.

1990 • 269 citations

A human nucleoporin-like protein that specifically interacts with HIV Rev

1995 • 268 citations

Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins.

1995 • 264 citations

A new family of yeast nuclear pore complex proteins.

1992 • 256 citations

Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.

1995 • 254 citations

Mechanisms of nuclear protein import

1995 • 241 citations

An RNA-export mediator with an essential nuclear export signal

1996 • 230 citations

Nuclear export signals and the fast track to the cytoplasm

1995 • 229 citations

Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast

1995 • 223 citations

A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic.

1993 • 211 citations

Identification of a Yeast Karyopherin Heterodimer That Targets Import Substrate to Mammalian Nuclear Pore Complexes

1995 • 210 citations

The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor.

1995 • 206 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins.

1996 • 177 citations

RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import.

1996 • 175 citations

GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function.

1996 • 168 citations

Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.

1995 • 167 citations

Role of the Nuclear Transport Factor p10 in Nuclear Import

1996 • 166 citations

The nuclear pore‐targeting complex binds to nuclear pores after association with a karyophile

1995 • 163 citations

A G protein involved in nucleocytoplasmic transport: the role of Ran

1994 • 159 citations

The yeast nuclear import receptor is required for mitosis.

1995 • 153 citations

Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.

1995 • 146 citations

A Karyophilic Protein Forms a Stable Complex with Cytoplasmic Components Prior to Nuclear Pore Binding

1995 • 144 citations

NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure.

1994 • 140 citations

The Essential Yeast Nucleoporin NUP159 Is Located on the Cytoplasmic Side of the Nuclear Pore Complex and Serves in Karyopherin-mediated Binding of Transport Substrate

1995 • 139 citations

GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.

1995 • 139 citations

Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif

1994 • 134 citations

Cytosolic factors in nuclear transport: What's importin?

1994 • 133 citations

Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.

1995 • 128 citations

An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore.

1990 • 128 citations

Dynamic localization of the nuclear import receptor and its interactions with transport factors.

1996 • 127 citations

The Nuclear Transport Factor Karyopherin β Binds Stoichiometrically to Ran-GTP and Inhibits the Ran GTPase Activating Protein

1996 • 123 citations

Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.

1996 • 110 citations

HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs

1996 • 106 citations

Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.

1996 • 105 citations

Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.

1990 • 104 citations

Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97.

1996 • 80 citations

Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway.

1996 • 77 citations

Characterization of an Essential Orc2p-Associated Factor That Plays a Role in DNA Replication

1996 • 69 citations

Ran Binding Domains Promote the Interaction of Ran with p97/β-Karyopherin, Linking the Docking and Translocation Steps of Nuclear Import

1996 • 54 citations

Defining the essential functional regions of the nucleoporin Nup145p

1997 • 42 citations

Nucleocytoplasmic transport: factors and mechanisms

1995 • 38 citations