Studies on Nucleotide and Receptor Regulation of G_iProteins: Effects of Pertussis Toxin

Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction

1973 • 12,522 citations

A highly sensitive adenylate cyclase assay

1974 • 4,213 citations

A ternary complex model explains the agonist-specific binding properties of the adenylate cyclase-coupled beta-adrenergic receptor.

1980 • 1,579 citations

The βγ subunits of GTP-binding proteins activate the muscarinic K+ channel in heart

1987 • 1,186 citations

Mechanism of cholera toxin action: Covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system

1978 • 902 citations

GTP-binding proteins couple cardiac muscarinic receptors to a K channel

1985 • 868 citations

Direct modification of the membrane adenylate cyclase system by islet-activating protein due to ADP-ribosylation of a membrane protein.

1982 • 808 citations

Rhodopsin content in the outer segment membranes of bovine and frog retinal rods

1974 • 730 citations

ADP ribosylation of the specific membrane protein of C6 cells by islet-activating protein associated with modification of adenylate cyclase activity.

1982 • 600 citations

Identification of the predominant substrate for ADP-ribosylation by islet activating protein.

1983 • 583 citations

A quantitative analysis of beta-adrenergic receptor interactions: resolution of high and low affinity states of the receptor by computer modeling of ligand binding data.

1980 • 545 citations

Purification and properties of the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase.

1984 • 498 citations

Relationship between the beta-adrenergic receptor and adenylate cyclase.

1977 • 497 citations

The Glucagon-sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver

1971 • 487 citations

Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site.

1985 • 457 citations

Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells.

1986 • 424 citations

Mechanism of adenylate cyclase activation through the beta-adrenergic receptor: catecholamine-induced displacement of bound GDP by GTP.

1978 • 372 citations

Reconstitution of resolved muscarinic cholinergic receptors with purified GTP-binding proteins.

1985 • 314 citations

The G protein-gated atrial K+ channel is stimulated by three distinct GIα-subunits

1988 • 306 citations

Direct evidence for involvement of a guanine nucleotide-binding protein in chemotactic peptide-stimulated formation of inositol bisphosphate and trisphosphate in differentiated human leukemic (HL-60) cells. Reconstitution with Gi or Go of the plasma membranes ADP-ribosylated by pertussis toxin.

1986 • 276 citations

Selection of a Variant Lymphoma Cell Deficient in Adenylate Cyclase

1975 • 276 citations

Pertussis toxin. Affinity purification of a new ADP-ribosyltransferase.

1983 • 268 citations

G protein multiplicity in eukaryotic signal transduction systems

1988 • 267 citations

Direct G protein gating of ion channels

1988 • 258 citations

Islet-activating protein. A modifier of receptor-mediated regulation of rat islet adenylate cyclase.

1981 • 257 citations

Mammalian .beta.2-adrenergic receptor: reconstitution of functional interactions between pure receptor and pure stimulatory nucleotide binding protein of the adenylate cyclase system

1984 • 249 citations

Hormone-stimulated desensitization of hormone-dependent adenylyl cyclase. Dual action of luteninizing hormone on pig graafian follicle membranes.

1976 • 244 citations

Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclases. Purification of the human erythrocyte proteins without the use of activating regulatory ligands.

1984 • 242 citations

Reconstitution of catecholamine-stimulated guanosine triphosphatase activity

1983 • 213 citations

Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.

1985 • 213 citations

Functional reconstitution of the alpha 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles.

1986 • 199 citations

A new GTP‐binding protein in brain tissues serving as the specific substrate of islet‐activating protein, pertussis toxin

1987 • 189 citations

ADP-ribosylation of adenylate cyclase by pertussis toxin. Effects on inhibitory agonist binding.

1984 • 189 citations

Reconstitution of Somatostatin and Muscarinic Receptor Mediated Stimulation of K⁺Channels by Isolated G_KProtein in Clonal Rat Anterior Pituitary Cell Membranes *

1987 • 188 citations

Identification of the GTP-binding protein encoded by Gi3 complementary DNA.

1988 • 169 citations

Functional interaction of purified muscarinic receptors with purified inhibitory guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.

1986 • 161 citations

Relationships within the family of GTP-binding proteins isolated from bovine central nervous system.

1985 • 160 citations

Signal transduction by G proteins

1990 • 159 citations

Association of the N-formyl-Met-Leu-Phe receptor in human neutrophils with a GTP-binding protein sensitive to pertussis toxin.

1985 • 159 citations

Guanine nucleotide inhibition of cyc- S49 mouse lymphoma cell membrane adenylyl cyclase.

1982 • 151 citations

Effects of guanine nucleotides and Mg on human erythrocyte Ni and Ns, the regulatory components of adenylyl cyclase.

1984 • 144 citations

Chromatographic resolution and immunologic identification of the alpha 40 and alpha 41 subunits of guanine nucleotide-binding regulatory proteins from bovine brain.

1988 • 144 citations

The Somatostatin Receptor Is Directly Coupled to Adenylate Cyclase in GH4Ci Pituitary Cell Membranes*

1984 • 130 citations

[3H]GDP release from rat and hamster adipocyte membranes independently linked to receptors involved in activation or inhibition of adenylate cyclase. Differential susceptibility to two bacterial toxins.

1984 • 116 citations

G-protein distribution in canine cardiac sarcoplasmic reticulum and sarcolemma: Comparison to rabbit skeletal muscle membranes and to brain and erythrocyte G-proteins

1987 • 115 citations

Guanine nucleotide regulation of a mammalian myocardial muscarinic receptor system. Evidence for homo- and heterotropic cooperativity in ligand binding analyzed by computer-assisted curve fitting.

1985 • 113 citations

Alpha i-3 cDNA encodes the alpha subunit of Gk, the stimulatory G protein of receptor-regulated K+ channels.

1988 • 104 citations

Stereochemistry of the guanyl nucleotide binding site of transducin probed by phosphorothioate analogs of GTP and GDP

1985 • 102 citations

Functional reconstitution of beta-adrenergic receptors and the stimulatory GTP-binding protein of adenylate cyclase.

1982 • 100 citations

Transient and steady state kinetics of the interaction of guanyl nucleotides with the adenylyl cyclase system from rat liver plasma membranes. Interpretation in terms of a simple two-state model.

1980 • 92 citations

GTP hydrolysis by pure Ni, the inhibitory regulatory component of adenylyl cyclases.

1984 • 89 citations

Differential effect of pertussis toxin on the affinity state for agonists of renal alpha 1- and alpha 2- adrenoceptors.

1984 • 79 citations

Guanine nucleotide-sensitive, high affinity binding of carbachol to muscarinic cholinergic receptors of 1321N1 astrocytoma cells is insensitive to pertussis toxin.

1985 • 78 citations

Hormone receptor-mediated stimulation of adenylyl cyclase systems. Nucleotide effects and analysis in terms of a simple two-state model for the basic receptor-affected enzyme.

1980 • 72 citations

Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates.

1987 • 66 citations

Reconstitution of the purified porcine atrial muscarinic acetylcholine receptor with purified porcine atrial inhibitory guanine nucleotide binding protein

1987 • 63 citations

Adenosine Diphosphate Ribosylation of G Proteins by Pertussis and Cholera Toxin in Isolated Membranes. Different Requirements for and Effects of Guanine Nucleotides and Mg²⁺

1987 • 61 citations

Adenine nucleotides directly stimulate pertussis toxin.

1985 • 61 citations

Regulation of glucagon receptor binding. Lack of effect of Mg and preferential role for GDP.

1985 • 48 citations

Functional uncoupling of muscarinic receptors from adenylate cyclase in rat cardiac membranes by the active component of islet-activating protein, pertussis toxin.

1983 • 47 citations

Modulation of muscarinic cholinergic receptor affinity for antagonists in rat heart.

1984 • 43 citations

The interaction of nucleotides with pertussis toxin. Direct evidence for a nucleotide binding site on the toxin regulating the rate of ADP-ribosylation of Ni, the inhibitory regulatory component of adenylyl cyclase.

1986 • 39 citations

Molecular Basis of Regulation of Ionic Channels by G Proteins

1989 • 24 citations

Updated Protocols and Comments on the Purification without Use of Activating Ligands of the Coupling Proteins N_sand N_iof the Hormone Sensitive Adenylyl Cyclase

1984 • 23 citations

Receptor-Effector Coupling by G Proteins: Purification of Human Erythrocyte Gi-2 and Gi-3 and Analysis of Effector Regulation Using Recombinant Subunits Synthesized in Escherichia coli

1988 • 17 citations