Functional reconstitution of the alpha 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.

1984 • 1,300 citations

Purification and properties of the inhibitory guanine nucleotide regulatory unit of brain adenylate cyclase.

1984 • 585 citations

Validation and statistical analysis of a computer modeling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes.

1982 • 573 citations

Purification and properties of the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase.

1984 • 498 citations

Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits.

1983 • 492 citations

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution of the activated 45,000-dalton (alpha) subunit.

1983 • 380 citations

Stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins

1983 • 309 citations

The regulatory components of adenylate cyclase and transducin. A family of structurally homologous guanine nucleotide-binding proteins.

1983 • 304 citations

Mammalian .beta.2-adrenergic receptor: reconstitution of functional interactions between pure receptor and pure stimulatory nucleotide binding protein of the adenylate cyclase system

1984 • 249 citations

Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclases. Purification of the human erythrocyte proteins without the use of activating regulatory ligands.

1984 • 242 citations

Pertussis toxin substrate, the putative Ni component of adenylyl cyclases, is an alpha beta heterodimer regulated by guanine nucleotide and magnesium.

1983 • 237 citations

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution, activity, and properties of the 35,000-dalton (beta) subunit.

1983 • 237 citations

Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles.

1985 • 213 citations

Reconstitution of catecholamine-stimulated guanosine triphosphatase activity

1983 • 213 citations

[45] ADP-ribosylation of membrane components by pertussis and cholera toxin

1985 • 209 citations

Mammalian .beta.2-adrenergic receptor: purification and characterization

1984 • 207 citations

Antisera against a guanine nucleotide binding protein from retina cross-react with the beta subunit of the adenylyl cyclase-associated guanine nucleotide binding proteins, Ns and Ni.

1985 • 160 citations

Reconstitution of catecholamine-stimulated binding of guanosine 5'-O-(3-thiotriphosphate) to the stimulatory GTP-binding protein of adenylate cyclase

1984 • 136 citations

Functional reconstitution of purified muscarinic receptors and inhibitory guanine nucleotide regulatory protein

1985 • 119 citations

The beta 1-adrenergic receptor of the turkey erythrocyte. Molecular heterogeneity revealed by purification and photoaffinity labeling.

1982 • 117 citations

Effects of guanyl nucleotides and rhodopsin on ADP-ribosylation of the inhibitory GTP-binding component of adenylate cyclase by pertussis toxin.

1984 • 100 citations

A role for Ni in the hormonal stimulation of adenylate cyclase

1985 • 99 citations

Antibodies against the carboxyl-terminal 5-kDa peptide of the alpha subunit of transducin crossreact with the 40-kDa but not the 39-kDa guanine nucleotide binding protein from brain.

1985 • 98 citations

The beta-adrenergic receptor: rapid purification and covalent labeling by photoaffinity crosslinking.

1982 • 76 citations

Purification and characterization of the mammalian .beta.2-adrenergic receptor

1983 • 66 citations

Organization of rhodopsin in photoreceptor membranes. 1. Proteolysis of bovine rhodopsin in native membranes and the distribution of sulfhydryl groups in the fragments

1978 • 65 citations

The fat cell beta-adrenergic receptor. Purification and characterization of a mammalian beta 1-adrenergic receptor.

1984 • 59 citations

Antibodies against a retinal guanine nucleotide‐binding protein cross‐react with a single plasma membrane protein in non‐retinal tissues

1984 • 51 citations

Transducin and the inhibitory nucleotide regulatory protein inhibit the stimulatory nucleotide regulatory protein mediated stimulation of adenylate cyclase in phospholipid vesicle systems

1985 • 43 citations

Identification of the subunit-binding site of alpha 2-adrenergic receptors using [3H]phenoxybenzamine.

1984 • 29 citations

Affinity chromatography of human platelet α ₂ -adrenergic receptors

1982 • 27 citations

Updated Protocols and Comments on the Purification without Use of Activating Ligands of the Coupling Proteins N_sand N_iof the Hormone Sensitive Adenylyl Cyclase

1984 • 23 citations