G-protein-effector coupling: A real-time light-scattering assay for transducin-phosphodiesterase interaction

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Cyclic GMP Cascade of Vision

1986 • 1,168 citations

Flow of information in the light-triggered cyclic nucleotide cascade of vision.

1981 • 740 citations

A quantitative account of the activation steps involved in phototransduction in amphibian photoreceptors.

1992 • 632 citations

Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits.

1983 • 492 citations

Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments.

1979 • 448 citations

Activation of pigeon erythrocyte membrane adenylate cyclase by guanylnucleotide analogues and separation of a nucleotide binding protein.

1975 • 337 citations

Purification and characterization of the gamma regulatory subunit of the cyclic GMP phosphodiesterase from retinal rod outer segments.

1982 • 336 citations

Molecular mechanism of visual transduction

1989 • 312 citations

THE MOLECULAR MECHANISM OF VISUAL EXCITATION AND ITS RELATION TO THE STRUCTURE AND COMPOSITION OF THE ROD OUTER SEGMENT

1987 • 264 citations

The isolation and purification of osmotically intact discs from retinal rod outer segments

1975 • 248 citations

Interactions between photoexcited rhodopsin and GTP-binding protein: kinetic and stoichiometric analyses from light-scattering changes.

1981 • 247 citations

cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.

1988 • 246 citations

The control of phosphodiesterase in rod disk membranes: Kinetics, possible mechanisms and significance for vision

1979 • 224 citations

Millisecond activation of transducin in the cyclic nucleotide cascade of vision

1984 • 211 citations

Reciprocal control of retinal rod cyclic GMP phosphodiesterase by its γ subunit and transducin

1986 • 151 citations

Subunit stoichiometry of retinal rod cGMP phosphodiesterase

1990 • 138 citations

[72] Real time assay of rod disk membrane cGMP phosphodiesterase and its controller enzymes

1982 • 125 citations

Reciprocal effects of an inhibitory factor on catalytic activity and noncatalytic cGMP binding sites of rod phosphodiesterase.

1982 • 105 citations

Kinetics and components of the flash photocurrent of isolated retinal rods of the larval salamander, Ambystoma tigrinum.

1987 • 102 citations

Activation of retinal rod cyclic GMP‐phosphodiesterase by transducin: Characterization of the complex formed by phosphodiesterase inhibitor and transducin α‐subunit

1986 • 102 citations

Deactivation kinetics of the transduction cascade of vision.

1991 • 101 citations

Activation of cGMP phosphodiesterase in retinal rods: mechanism of interaction with the GTP-binding protein (transducin)

1989 • 94 citations

Kinetic studies suggest that light-activated cyclic GMP phosphodiesterase is a complex with G-protein subunits

1986 • 93 citations

Activation mechanism of retinal rod cyclic GMP phosphodiesterase probed by fluorescein-labeled inhibitory subunit

1990 • 86 citations

Sites of interaction between rod G-protein alpha-subunit and cGMP-phosphodiesterase gamma-subunit. Implications for the phosphodiesterase activation mechanism.

1992 • 81 citations

Two-site high-affinity interaction between inhibitory and catalytic subunits of rod cyclic GMP phosphodiesterase

1992 • 79 citations

The membrane binding domain of rod cGMP phosphodiesterase is posttranslationally modified by methyl esterification at a C-terminal cysteine.

1989 • 75 citations

Activation and solubilization of the retinal cGMP‐specific phosphodiesterase by limited proteolysis

1991 • 67 citations

Functional regions of the inhibitory subunit of retinal rod cGMP phosphodiesterase identified by site-specific mutagenesis and fluorescence spectroscopy

1992 • 66 citations

Watching G proteins at work

1990 • 64 citations

The cGMP phosphodiesterase-transducin complex of retinal rods. Membrane binding and subunits interactions.

1992 • 62 citations

Membrane stimulation of cGMP phosphodiesterase activation by transducin: comparison of phospholipid bilayers to rod outer segment membranes

1992 • 61 citations

Comparison of the phosphodiesterase inhibitory subunit interactions of frog and bovine rod outer segments

1989 • 53 citations

Kinetic study on the equilibrium between membrane-bound and free photoreceptor G-protein

1987 • 50 citations

Evidence for two functionally different membrane fractions in bovine retinal rod outer segments.

1988 • 43 citations

An Antibody-induced Enhancement of the Transducin-stimulated Cyclic GMP Phosphodiesterase Activity

1989 • 41 citations

Displacement of rhodopsin by GDP from three-loop interaction with transducin depends critically on the diphosphate beta-position.

1990 • 41 citations

Rod outer segment phosphodiesterase binding and activation in reconstituted membranes

1984 • 37 citations

The effect of the γ-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme

1990 • 33 citations

Light and GTP dependence of transducin solubility in retinal rods

1988 • 27 citations

Binding of the γ-subunit of retinal rod-outer-segment phosphodiesterase with both transducin and the catalytic subunits of phosphodiesterase

1990 • 21 citations

Resonance energy transfer as a direct monitor of GTP-binding protein-effector interactions: activated .alpha.-transducin binding to the cGMP phosphodiesterase in the bovine phototransduction cascade

1991 • 20 citations

cGMP- and phosphodiesterase-dependent light-scattering changes in rod disk membrane vesicles: relationship to disk vesicle-disk vesicle aggregation

1985 • 17 citations

Light- and nucleotide-dependent binding of phosphodiesterase to rod disk membranes: correlation with light-scattering changes and vesicle aggregation

1986 • 14 citations

The amplified P‐signal, an extremely photosensitive light scattering signal from rod outer segments, which is not affected by pre‐activation of phosphodiesterase with Gα‐GTP‐γ‐S

1987 • 11 citations

A functional link between the dark Mg‐ATPase activity and the light‐induced enzymatic cascade in rod outer segments

1986 • 10 citations

cGMP phosphodiesterase dependent light-induced scattering changes in suspensions of retinal disk membranes

1992 • 7 citations

Rhodopsin-detergent micelles aggregate upon activation of cyclic guanosine monophosphate phosphodiesterase

1990 • 2 citations