[30] Pore-blocking toxins as probes of voltage-dependent channels

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

PCR protocols — A guide to methods and applications

1990 • 10,078 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Classical Electrostatics in Biology and Chemistry

1995 • 2,801 citations

Proteins: Structures and Molecular Properties

1986 • 2,694 citations

Cation-π Interactions in Chemistry and Biology: A New View of Benzene, Phe, Tyr, and Trp

1996 • 2,561 citations

The action of calcium on the electrical properties of squid axons

1957 • 1,853 citations

High-Resolution Epitope Mapping of hGH-Receptor Interactions by Alanine-Scanning Mutagenesis

1989 • 1,357 citations

Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence

1984 • 1,339 citations

Proteins. Structures and molecular properties

1984 • 1,247 citations

Structural parts involved in activation and inactivation of the sodium channel

1989 • 1,189 citations

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

Structure and Function of Voltage-Sensitive Ion Channels

1988 • 1,171 citations

The folding of an enzyme

1992 • 931 citations

Existence of distinct sodium channel messenger RNAs in rat brain

1986 • 909 citations

Molecular Determinants of State-Dependent Block of Na ⁺ Channels by Local Anesthetics

1994 • 829 citations

Calcium channel characteristics conferred on the sodium channel by single mutations

1992 • 776 citations

CALCIUM CHANNEL DIVERSITY AND NEUROTRANSMITTER RELEASE: The ω-Conotoxins and ω-Agatoxins

1994 • 734 citations

Mapping the transition state and pathway of protein folding by protein engineering

1989 • 699 citations

Acetylcholine Receptor Channel Structure Probed in Cysteine-Substitution Mutants

1992 • 668 citations

A Mechanism for Ion Selectivity in Potassium Channels: Computational Studies of Cation-π Interactions

1993 • 623 citations

Acetylcholine Binding by a Synthetic Receptor: Implications for Biological Recognition

1990 • 608 citations

Molecular Basis of Charge Movement in Voltage-Gated Sodium Channels

1996 • 603 citations

Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH

1975 • 599 citations

Direct Physical Measure of Conformational Rearrangement Underlying Potassium Channel Gating

1996 • 598 citations

Mutations Affecting Tea Blockade and Ion Permeation in Voltage-activated K ⁺ Channels

1990 • 569 citations

Primary structure and functional expression of a mammalian skeletal muscle sodium channel

1989 • 561 citations

Energetics of protein-protein interactions: Analysis ofthe Barnase-Barstar interface by single mutations and double mutant cycles

1995 • 559 citations

Diversity of Conus Neuropeptides

1990 • 537 citations

Expression of functional sodium channels from cloned cDNA

1986 • 537 citations

Transmembrane Movement of the Shaker K+ Channel S4

1996 • 496 citations

Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel II

1991 • 460 citations

The aromatic binding site for tetraethylammonium ion on potassium channels

1992 • 446 citations

Revealing the Architecture of a K ⁺ Channel Pore Through Mutant Cycles with a Peptide Inhibitor

1995 • 434 citations

Strength and co-operativity of contributions of surface salt bridges to protein stability

1990 • 415 citations

Conus geographus toxins that discriminate between neuronal and muscle sodium channels.

1985 • 402 citations

Evidence for voltage-dependent S4 movement in sodium channels

1995 • 396 citations

A Mutant of TTX-Resistant Cardiac Sodium Channels with TTX-Sensitive Properties

1992 • 387 citations

Refined Structure of Charybdotoxin: Common Motifs in Scorpion Toxins and Insect Defensins

1991 • 367 citations

Mechanism of charybdotoxin block of the high-conductance, Ca2+-activated K+ channel.

1988 • 355 citations

Mutant Potassium Channels with Altered Binding of Charybdotoxin, a Pore-Blocking Peptide Inhibitor

1989 • 351 citations

Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles

1990 • 350 citations

A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

1989 • 336 citations

Strategy for analysing the co-operativity of intramolecular interactions in peptides and proteins

1990 • 329 citations

SOLID PHASE SYNTHESIS WITHOUT REPETITIVE ACIDOLYSIS

1979 • 308 citations

Primary structure and expression of a sodium channel characteristic of denervated and immature rat skeletal muscle

1990 • 297 citations

The receptor for tetrodotoxin and saxitoxin. A structural hypothesis

1975 • 295 citations

Spatial Localization of the K+ Channel Selectivity Filter by Mutant Cycle–Based Structure Analysis

1996 • 288 citations

A mild procedure for solid phase peptide synthesis: use of fluorenylmethoxycarbonylamino-acids

1978 • 287 citations

The charybdotoxin receptor of a Shaker K+ channel: Peptide and channel residues mediating molecular recognition

1994 • 286 citations

Discrete Ba2+ block as a probe of ion occupancy and pore structure in the high-conductance Ca2+ -activated K+ channel.

1988 • 285 citations

Molecular Localization of an Ion-Binding Site Within the Pore of Mammalian Sodium Channels

1992 • 281 citations

A structural model of the tetrodotoxin and saxitoxin binding site of the Na+ channel

1994 • 274 citations

Peptidylglycine α‐amidating monooxygenase: A multifunctional protein with catalytic, processing, and routing domains

1993 • 263 citations

An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantula

1995 • 254 citations

On the structural basis for ionic selectivity among Na+, K+, and Ca2+ in the voltage-gated sodium channel

1996 • 254 citations

Topology of the pore-region of a K+ channel revealed by the NMR-derived structures of scorpion toxins

1995 • 253 citations

Genomic Organization and Deduced Amino Acid Sequence of a Putative Sodium Channel Gene in Drosophila

1987 • 244 citations

Pore loops: An emerging theme in ion channel structure

1995 • 237 citations

Potassium blocks barium permeation through a calcium-activated potassium channel.

1988 • 227 citations

Mapping the receptor site for charybdotoxin, a pore-blocking potassium channel inhibitor

1990 • 220 citations

The binding of labelled tetrodotoxin to non‐myelinated nerve fibres

1972 • 206 citations

The adult rat brain beta 1 subunit modifies activation and inactivation gating of multiple sodium channel alpha subunits

1994 • 200 citations

Single sodium channels from rat brain incorporated into planar lipid bilayer membranes

1983 • 186 citations

Silver as a Probe of Pore-Forming Residues in a Potassium Channel

1995 • 182 citations

A Critical Role for Transmembrane Segment IVS6 of the Sodium Channel α Subunit in Fast Inactivation

1995 • 175 citations

Interaction of charybdotoxin with permeant ions inside the pore of a K+ channel

1992 • 175 citations

Three‐dimensional structure of natural charybdotoxin in aqueous solution by ¹H‐NMR Charybdotoxin possesses a structural motif found in other scorpion toxins

1991 • 175 citations

Intimations of potassium channel structure from a complete functional map of the molecular surface of charybdotoxin

1994 • 172 citations

Functional Consequences of Sulfhydryl Modification in the Pore-Forming Subunits of Cardiovascular Ca ²⁺ and Na ⁺ Channels

1995 • 164 citations

Batrachotoxin-activated Na+ channels in planar lipid bilayers. Competition of tetrodotoxin block by Na+.

1984 • 161 citations

Contribution of Sialic Acid to the Voltage Dependence of Sodium Channel Gating

1997 • 161 citations

Mapping function to structure in a channel-blocking peptide: electrostatic mutants of charybdotoxin

1992 • 155 citations

Conotoxins.

1991 • 150 citations

Analysis of side-chain organization on a refined model of charybdotoxin: structural and functional implications

1992 • 148 citations

A New Family of Conotoxins That Blocks Voltage-gated Sodium Channels

1995 • 145 citations

μl Na+ channels expressed transiently in human embryonic kidney cells: Biochemical and biophysical properties

1992 • 137 citations

Voltage-dependent blockade of muscle Na+ channels by guanidinium toxins.

1984 • 134 citations

A Strongly Interacting Pair of Residues on the Contact Surface of Charybdotoxin and a Shaker K+ Channel

1996 • 131 citations

The binding of labelled saxitoxin to the sodium channels in nerve membranes

1973 • 128 citations

The Signature Sequence of Voltage-gated Potassium Channels Projects into the External Vestibule

1996 • 128 citations

Interfacial Solutions of the Poisson-Boltzmann Equation

1961 • 123 citations

Molecular basis for pharmacological differences between brain and cardiac sodium channels

1992 • 111 citations

Active site of mu-conotoxin GIIIA, a peptide blocker of muscle sodium channels.

1991 • 108 citations

Depth Asymmetries of the Pore-Lining Segments of the Na+ Channel Revealed by Cysteine Mutagenesis

1996 • 107 citations

Action of derivatives of .mu.-conotoxin GIIIA on sodium channels. Single amino acid substitutions in the toxin separately affect association and dissociation rates

1992 • 104 citations

A mu-conotoxin-insensitive Na+ channel mutant: possible localization of a binding site at the outer vestibule

1995 • 100 citations

Effects of membrane surface charge and calcium on the gating of rat brain sodium channels in planar bilayers.

1988 • 93 citations

Three-Dimensional Solution Structure of μ-Conotoxin GIIIB, a Specific Blocker of Skeletal Muscle Sodium Channels^,

1996 • 92 citations

Interactions between a Pore-Blocking Peptide and the Voltage Sensor of the Sodium Channel: An Electrostatic Approach to Channel Geometry

1996 • 92 citations

Predominant Interactions between μ-Conotoxin Arg-13 and the Skeletal Muscle Na⁺Channel Localized by Mutant Cycle Analysis

1998 • 87 citations

Characterization of high affinity binding sites for charybdotoxin in synaptic plasma membranes from rat brain. Evidence for a direct association with an inactivating, voltage-dependent, potassium channel.

1990 • 87 citations

Voltage-dependent block by saxitoxin of sodium channels incorporated into planar lipid bilayers

1984 • 86 citations

Structure-activity relationships of .mu.-conotoxin GIIIA: structure determination of active and inactive sodium channel blocker peptides by NMR and simulated annealing calculations

1992 • 84 citations

Block of Ca channels in rat central neurons by the spider toxin omega- Aga-IIIA

1994 • 82 citations

Simple shifts in the voltage dependence of sodium channel gating caused by divalent cations.

1983 • 82 citations

Electrified Interfaces in Physics, Chemistry and Biology

1992 • 79 citations

Chimeric study of sodium channels from rat skeletal and cardiac muscle

1992 • 78 citations

Tertiary structure of conotoxin GIIIA in aqueous solution

1991 • 78 citations

Tetrodotoxin receptors in plasma membranes isolated from lobster nerve fibers

1973 • 74 citations

A point mutation in a Shaker K+ channel changes its charybdotoxin binding site from low to high affinity

1992 • 71 citations

Structure, function and expression of voltage-dependent sodium channels

1993 • 70 citations

Specific inhibition of [3H] saxitoxin binding to skeletal muscle sodium channels by geographutoxin II, a polypeptide channel blocker.

1986 • 69 citations

Altered Ionic Selectivity of the Sodium Channel Revealed by Cysteine Mutations within the Pore

1997 • 69 citations

P-loop Flexibility in Na+ Channel Pores Revealed by Single- and Double-cysteine Replacements

1997 • 63 citations

Isochannels and Blocking Modes of Voltage‐Dependent Sodium Channels^a

1986 • 62 citations

Single point mutations of the sodium channel drastically reduce the pore permeability without preventing its gating

1991 • 61 citations

Molecular motions within the pore of voltage-dependent sodium channels

1997 • 60 citations

A novel sodium channel inhibitor from Conus geographus: purification, structure, and pharmacological properties

1988 • 60 citations

The rates of saxitoxin action and of saxitoxin-tetrodotoxin interaction at the node of Ranvier

1975 • 54 citations

The ?I skeletal muscle sodium channel: Mutation E403Q eliminates sensitivity to tetrodotoxin but not to ?-conotoxins GIIIA and GIIIB

1994 • 53 citations

Topology of the P segments in the sodium channel pore revealed by cysteine mutagenesis

1997 • 53 citations

Specificity for block by saxitoxin and divalent cations at a residue which determines sensitivity of sodium channel subtypes to guanidinium toxins.

1995 • 51 citations

Mechanisms of sodium/calcium selectivity in sodium channels probed by cysteine mutagenesis and sulfhydryl modification

1997 • 46 citations

Molecular properties of a superfamily of plasma-membrane cation channels

1994 • 45 citations

Characterizing the mu-conotoxin binding site on voltage-sensitive sodium channels with toxin analogs and channel mutations.

1995 • 44 citations

Modeling ion permeation through batrachotoxin-modified Na+ channels from rat skeletal muscle with a multi-ion pore

1992 • 41 citations

Diffusion-controlled binding of a peptide neurotoxin to its potassium channel receptor

1990 • 40 citations

From Ion Channels to Cell-to-Cell Conversations

1997 • 32 citations

Cloning and expression of wild-type and mutant forms of the cardiotonic polypeptide anthopleurin B.

1992 • 32 citations

Fine structure-activity analysis of mutations at position 51 of tyrosyl-tRNA synthetase

1985 • 31 citations

Molecular basis of permeation in voltage-gated ion channels.

1993 • 28 citations

Differential effects of sulfhydryl reagents on saxitoxin and tetrodotoxin block of voltage-dependent Na channels

1994 • 27 citations

A method for the site-directed mono- and multi-mutagenesis of double-stranded DNA

1993 • 26 citations

Mapping hydrophobic residues of the interaction surface of charybdotoxin

1992 • 25 citations

Solvation, water permeation, and ionic selectivity of a putative model for the pore region of the voltage-gated sodium channel

1996 • 24 citations

Kinetics of TTX‐STX Block of Sodium Channels^a

1986 • 23 citations

Post-repolarization block of cloned sodium channels by saxitoxin: the contribution of pore-region amino acids

1994 • 19 citations

Synthesis and characterization of μ‐conotoxin IIIa

1989 • 19 citations

Structure and Function of Sodium Channels

1993 • 18 citations

Rapid Site-Directed Mutagenesis by a Method That Selects for Full Length Mutated DNA

1996 • 6 citations

Synthesis and characterization of an N‐terminal‐specific ¹²⁵I‐photoaffinity derivative of μ‐Conotoxin GIIIA which binds to the voltage‐dependent sodium channel

1990 • 6 citations

Molecular Aspects of Ion Permeation through Channels

1993 • 4 citations