Biomarkers of Oxidative Damage in Human Disease

Free radicals in biology and medicine

1991 • 17,840 citations

Oxidants, oxidative stress and the biology of ageing

2000 • 8,990 citations

Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes

1991 • 6,550 citations

Neurodegenerative diseases and oxidative stress

2004 • 3,422 citations

Role of Oxidative Modifications in Atherosclerosis

2004 • 2,558 citations

Protein carbonyl groups as biomarkers of oxidative stress

2003 • 2,210 citations

Measuring reactive species and oxidative damagein vivoand in cell culture: how should you do it and what do the results mean?

2004 • 2,159 citations

Oxidative stress in Parkinson's disease

2003 • 2,001 citations

Free radical-mediated oxidation of free amino acids and amino acid residues in proteins

2003 • 1,787 citations

Role of oxygen radicals in DNA damage and cancer incidence

2004 • 1,762 citations

Biochemistry and pathology of radical-mediated protein oxidation

1997 • 1,675 citations

The Role of Oxidative Stress in Carcinogenesis

2004 • 1,516 citations

Role of Free Radicals in the Neurodegenerative Diseases

2001 • 1,490 citations

The role of oxidized lipoproteins in atherogenesis

1996 • 1,382 citations

Oxidant signals and oxidative stress

2003 • 1,378 citations

The oxidative environment and protein damage

2004 • 1,285 citations

Effects of Long-term Vitamin E Supplementation on Cardiovascular Events and Cancer

2005 • 1,160 citations

Analysis of glutathione: implication in redox and detoxification

2003 • 1,126 citations

4-Hydroxy-2-nonenal: a product and mediator of oxidative stress

2003 • 1,119 citations

Use of antioxidant vitamins for the prevention of cardiovascular disease: meta-analysis of randomised trials

2003 • 1,094 citations

Measurement of F2-isoprostanes as an index of oxidative stress in vivo

2000 • 960 citations

Protein carbonylation in human diseases

2003 • 942 citations

Free radical-induced damage to DNA: mechanisms and measurement 1,2 1This article is part of a series of reviews on “Oxidative DNA Damage and Repair.” The full list of papers may be found on the homepage of the journal. 2Guest Editor: Miral Dizdaroglu

2002 • 915 citations

3-Chlorotyrosine, a specific marker of myeloperoxidase-catalyzed oxidation, is markedly elevated in low density lipoprotein isolated from human atherosclerotic intima.

1997 • 840 citations

Amyloid β-peptide (1-42)-induced Oxidative Stress and Neurotoxicity: Implications for Neurodegeneration in Alzheimer's Disease Brain. A Review

2002 • 764 citations

Increased prevalence of oxidant stress and inflammation in patients with moderate to severe chronic kidney disease

2004 • 760 citations

Isoprostanes: markers and mediators of oxidative stress

2004 • 740 citations

Evidence that amyloid beta-peptide-induced lipid peroxidation and its sequelae in Alzheimer’s disease brain contribute to neuronal death1

2002 • 700 citations

Biomarkers of Oxidative Stress Study II: Are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning?

2004 • 685 citations

Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations

2004 • 657 citations

Reactive nitrogen species in the chemical biology of inflammation

2004 • 657 citations

Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease

2004 • 654 citations

Carbonyl modified proteins in cellular regulation, aging, and disease2,3 2Guest Editor: Earl Stadtman 3This article is part of a series of reviews on “Oxidatively Modified Proteins in Aging and Disease.” The full list of papers may be found on the homepage of the journal.

2002 • 637 citations

Oxidative stress and nitration in neurodegeneration: Cause, effect, or association?

2003 • 634 citations

Decreased proteolysis caused by protein aggregates, inclusion bodies, plaques, lipofuscin, ceroid, and ‘aggresomes’ during oxidative stress, aging, and disease

2004 • 630 citations

Oxidative stress and nitration in neurodegeneration: Cause, effect, or association?

2003 • 626 citations

Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease

2004 • 623 citations

Reactive Oxygen-Mediated Protein Oxidation in Aging and Disease

1997 • 605 citations

Role of reactive aldehyde in cardiovascular diseases

2000 • 597 citations

Oxidants, antioxidants and the ischemic brain

2004 • 595 citations

Antioxidant Therapy in Acute Central Nervous System Injury: Current State

2002 • 567 citations

Increase of Brain Oxidative Stress in Mild Cognitive Impairment

2002 • 563 citations

Exhaled 8-Isoprostane as an In Vivo Biomarker of Lung Oxidative Stress in Patients with COPD and Healthy Smokers

2000 • 542 citations

Tyrosine nitration: Localisation, quantification, consequences for protein function and signal transduction

2001 • 504 citations

Biological selectivity and functional aspects of protein tyrosine nitration

2003 • 501 citations

A Tale of Two Controversies

2002 • 484 citations

Modification of histidine residues in proteins by reaction with 4-hydroxynonenal.

1992 • 481 citations

Selective degradation of oxidatively modified protein substrates by the proteasome

2003 • 457 citations

Association of Nitrotyrosine Levels With Cardiovascular Disease and Modulation by Statin Therapy

2003 • 453 citations

Stable markers of oxidant damage to proteins and their application in the study of human disease

1999 • 451 citations

Proteasome inhibition by paired helical filament‐tau in brains of patients with Alzheimer's disease

2003 • 447 citations

Quantification of Isoprostanes as Indices of Oxidant Stress and the Risk of Atherosclerosis in Humans

2005 • 447 citations

4-Hydroxy-2-Nonenal, a Specific Lipid Peroxidation Product, Is Elevated in Lungs of Patients with Chronic Obstructive Pulmonary Disease

2002 • 443 citations

The myeloperoxidase product hypochlorous acid oxidizes HDL in the human artery wall and impairs ABCA1-dependent cholesterol transport

2004 • 443 citations

Lipid peroxidation in aging brain and Alzheimer’s disease1,2 1Guest Editors: Mark A. Smith and George Perry 2This article is part of a series of reviews on “Causes and Consequences of Oxidative Stress in Alzheimer’s Disease.” The full list of papers may be found on the homepage of the journal.

2002 • 440 citations

Proteins as biomarkers of oxidative/nitrosative stress in diseases: The contribution of redox proteomics

2004 • 439 citations

Statins Promote Potent Systemic Antioxidant Effects Through Specific Inflammatory Pathways

2003 • 396 citations

Glutathione, iron and Parkinson’s disease

2002 • 395 citations

Increased levels of 4-hydroxynonenal and acrolein, neurotoxic markers of lipid peroxidation, in the brain in Mild Cognitive Impairment and early Alzheimer's disease

2005 • 377 citations

Protein Nitration in Cardiovascular Diseases

2002 • 372 citations

Proteomic method identifies proteins nitrated in vivo during inflammatory challenge

2001 • 353 citations

Increased 8,12-iso-iPF2?-VI in Alzheimer's disease: Correlation of a noninvasive index of lipid peroxidation with disease severity

2000 • 345 citations

Immunological characterization of urinary 8-epi-prostaglandin F2 alpha excretion in man.

1995 • 340 citations

Oxidation of Methionine Residues of Proteins: Biological Consequences

2003 • 324 citations

Isoprostanes as a biomarker of lipid peroxidation in humans: physiology, pharmacology and clinical implications

2002 • 323 citations

Biomarkers of lipid peroxidation, airway inflammation and asthma

2003 • 320 citations

4-Hydroxy-2-nonenal-mediated Impairment of Intracellular Proteolysis during Oxidative Stress

1999 • 320 citations

The importance of lipid-derived malondialdehyde in diabetes mellitus

2000 • 313 citations

Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells?

2004 • 306 citations

Proteasome inhibition by lipofuscin/ceroid during postmitotic aging of fibroblasts

2000 • 302 citations

Determination of Carbonyl Groups in Oxidized Proteins

2003 • 299 citations

Antioxidant responses to oxidant-mediated lung diseases

2002 • 294 citations

Biomarkers of some pulmonary diseases in exhaled breath

2002 • 288 citations

Eosinophils Are a Major Source of Nitric Oxide-Derived Oxidants in Severe Asthma: Characterization of Pathways Available to Eosinophils for Generating Reactive Nitrogen Species

2001 • 281 citations

Eosinophils generate brominating oxidants in allergen-induced asthma

2000 • 279 citations

S‐Glutathionylation: from redox regulation of protein functions to human diseases

2004 • 278 citations

The effect of dietary nitrate on salivary, plasma, and urinary nitrate metabolism in humans

2003 • 277 citations

A Causative Role for Redox Cycling of Myoglobin and Its Inhibition by Alkalinization in the Pathogenesis and Treatment of Rhabdomyolysis-induced Renal Failure

1998 • 271 citations

Effect of diet on cancer development: is oxidative DNA damage a biomarker?1,2 1This article is part of a series of reviews on “Oxidative DNA Damage and Repair.” The full list of papers may be found on the homepage of the journal. 2Guest Editor: Miral Dizdaroglu

2002 • 263 citations

Human Atherosclerotic Intima and Blood of Patients with Established Coronary Artery Disease Contain High Density Lipoprotein Damaged by Reactive Nitrogen Species

2004 • 261 citations

Proteolytic Degradation of Tyrosine Nitrated Proteins

2000 • 260 citations

Urinary 8-iso-Prostaglandin F _2α as a Risk Marker in Patients With Coronary Heart Disease

2004 • 260 citations

4-Hydroxynonenal As a Biological Signal: Molecular Basis and Pathophysiological Implications

1999 • 249 citations

Alcohol-induced generation of lipid peroxidation products in humans

1999 • 249 citations

Biomarkers

2002 • 248 citations

Neutrophils employ the myeloperoxidase system to generate antimicrobial brominating and chlorinating oxidants during sepsis

2001 • 248 citations

Oxidative Stress and Respiratory Muscle Dysfunction in Severe Chronic Obstructive Pulmonary Disease

2005 • 244 citations

Blood Glutathione Disulfide: In Vivo Factor or in Vitro Artifact?

2002 • 242 citations

Cerebrospinal fluid F₂‐isoprostane levels are increased in Alzheimer's disease

1998 • 241 citations

Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes

2005 • 237 citations

Tyrosine oxidation products: analysis and biological relevance

2003 • 228 citations

Isoprostanes: an overview and putative roles in pulmonary pathophysiology

2001 • 226 citations

Recent advances in the analysis of oxidized proteins

2003 • 221 citations

Exhaled 8-isoprostane as a new non-invasive biomarker of oxidative stress in cystic fibrosis

2000 • 220 citations

Nitrated and oxidized plasma proteins in smokers and lung cancer patients.

2001 • 212 citations

ReviewIsoprostanes: Novel Bioactive Products of Lipid Peroxidation

2003 • 210 citations

Changes in glutathione status and the antioxidant system in blood and in cancer cells associate with tumour growth in vivo

1999 • 206 citations

Intracellular metabolism of 4-hydroxynonenal

2003 • 206 citations

Myeloperoxidase and protein oxidation in cystic fibrosis

2000 • 205 citations

Kinetic Analysis of the Reactions of Hypobromous Acid with Protein Components: Implications for Cellular Damage and Use of 3-Bromotyrosine as a Marker of Oxidative Stress

2004 • 195 citations

Biomarkers of oxidative stress study

2004 • 194 citations

The isoprostanes in biology and medicine

2001 • 190 citations

Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation

2002 • 183 citations

Dityrosine as a product of oxidative stress and fluorescent probe

2003 • 178 citations

Inflammation and NO_x-induced nitration: Assay for 3-nitrotyrosine by HPLC with electrochemical detection

1997 • 175 citations

Trans -4-hydroxy-2-nonenal inhibits nucleotide excision repair in human cells: A possible mechanism for lipid peroxidation-induced carcinogenesis

2004 • 174 citations

Mass spectrometry for detection of 4‐hydroxy‐trans‐2‐nonenal (HNE) adducts with peptides and proteins

2004 • 174 citations

Genotoxic properties of 4-hydroxyalkenals and analogous aldehydes

1993 • 166 citations

Actin Glutathionylation Increases in Fibroblasts of Patients with Friedreich's Ataxia

2003 • 156 citations

Plasma proteins modified by tyrosine nitration in acute respiratory distress syndrome

2000 • 151 citations

Peripheral F₂‐isoprostanes and F₄‐neuroprostanes are not increased in Alzheimer's disease

2002 • 151 citations

Histidine and lysine as targets of oxidative modification

2003 • 150 citations

An improved HPLC measurement for GSH and GSSG in human blood

2003 • 146 citations

Myeloperoxidase and Protein Oxidation in the Airways of Young Children with Cystic Fibrosis

2004 • 144 citations

Protein oxidation and ageing

2001 • 143 citations

Hydroxyl radical generation during exercise increases mitochondrial protein oxidation and levels of urinary dityrosine

1999 • 140 citations

Fate of 4-hydroxynonenal in vivo: disposition and metabolic pathways

2003 • 138 citations

Analysis of free and protein-bound nitrotyrosine in human plasma by a gas chromatography/mass spectrometry method that avoids nitration artifacts

2000 • 135 citations

Proteasome inhibition by lipofuscin/ceroid during postmitotic aging of fibroblasts

2000 • 134 citations

Artifact-Free Quantification of Free 3-Chlorotyrosine, 3-Bromotyrosine, and 3-Nitrotyrosine in Human Plasma by Electron Capture–Negative Chemical Ionization Gas Chromatography Mass Spectrometry and Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

2002 • 133 citations

Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis

2005 • 131 citations

Mass Spectrometric Quantification of 3-Chlorotyrosine in Human Tissues with Attomole Sensitivity

1997 • 130 citations

Urinary isoprostane excretion is not confounded by the lipid content of the diet

1999 • 130 citations

Simultaneous determination of tyrosine, phenylalanine and deoxyguanosine oxidation products by liquid chromatography–tandem mass spectrometry as non-invasive biomarkers for oxidative damage

2003 • 129 citations

Protein carbonyl measurement by enzyme-linked immunosorbent assay

1999 • 128 citations

High Molecular Weight Neurofilament Proteins Are Physiological Substrates of Adduction by the Lipid Peroxidation Product Hydroxynonenal

2002 • 127 citations

Antioxidant Treatment in Alzheimer's Disease: Current State

2003 • 127 citations

Alzheimer-specific epitopes of tau represent lipid peroxidation-induced conformations

2004 • 124 citations

Supplementation of postmenopausal women with fish oil rich in eicosapentaenoic acid and docosahexaenoic acid is not associated with greater in vivo lipid peroxidation compared with oils rich in oleate and linoleate as assessed by plasma malondialdehyde and F2-isoprostanes

2000 • 121 citations

Abnormalities in Nitric Oxide and Its Derivatives in Lung Cancer

2005 • 121 citations

Damage to plasmid DNA by singlet oxygen and its protection

1993 • 121 citations

A review of approaches to the analysis of 3-nitrotyrosine

2003 • 120 citations

Blood glutathione decreases in chronic diseases

2000 • 119 citations

F2-isoprostanes as indices of lipid peroxidation in inflammatory diseases

2003 • 118 citations

Oxidized proteins as a marker of oxidative stress during coronary heart surgery

1999 • 117 citations

Chronic Angiotensin II Receptor Blockade Reduces (Intra)Renal Vascular Resistance in Patients with Type 2 Diabetes

2005 • 114 citations

Factors Regulating Isoprostane Formation In Vivo

2004 • 112 citations

F₂-Isoprostanes in Alzheimer and Other Neurodegenerative Diseases

2004 • 112 citations

Oxidized amino acids: culprits in human atherosclerosis and indicators of oxidative stress 1,2 1This article is part of a series of reviews on “Oxidatively Modified Proteins in Aging and Disease.” The full list of papers may be found on the homepage of the journal. 2Guest Editor: Earl Stadtman

2002 • 111 citations

Detection of HOCl-mediated protein oxidation products in the extracellular matrix of human atherosclerotic plaques

2003 • 110 citations

Eosinophil peroxidase produces hypobromous acid in the airways of stable asthmatics

2002 • 108 citations

Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis

2005 • 102 citations

Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection

2002 • 101 citations

Divergence in urinary 8-iso-PGF2α (iPF2α-III, 15-F2t-IsoP) levels from gas chromatography–tandem mass spectrometry quantification after thin-layer chromatography and immunoaffinity column chromatography reveals heterogeneity of 8-iso-PGF2α

2003 • 100 citations

Quantification of 3-nitrotyrosine in biological tissues and fluids: Generating valid results by eliminating artifactual formation

2000 • 98 citations

The human pituitary nitroproteome: detection of nitrotyrosyl-proteins with two-dimensional Western blotting, and amino acid sequence determination with mass spectrometry

2004 • 97 citations

Increased oxidative stress in kwashiorkor

2000 • 96 citations

Recent methodological advances in the mass spectrometric analysis of free and protein-associated 3-nitrotyrosine in human plasma

2004 • 92 citations

Increasing plasma fatty acids elevates F2-isoprostanes in humans: implications for the cardiovascular risk factor cluster

2002 • 90 citations

Fast liquid chromatography–mass spectrometry glutathione measurement in whole blood: micromolar GSSG is a sample preparation artifact

2003 • 88 citations

Oxidized amino acids in the urine of aging rats: potential markers for assessing oxidative stress in vivo

1999 • 87 citations

Blood glutathione and cysteine changes in cardiovascular disease

2000 • 86 citations

Nebivolol decreases systemic oxidative stress in healthy volunteers

2000 • 85 citations

Gas Chromatographic–Tandem Mass Spectrometric Quantification of Free 3-Nitrotyrosine in Human Plasma at the Basal State

1999 • 84 citations

Urinary aldehydes as indicators of lipid peroxidation in vivo

2000 • 81 citations

Covalent modification of actin by 4‐hydroxy‐trans‐2‐nonenal (HNE): LC‐ESI‐MS/MS evidence for Cys374 Michael adduction

2005 • 79 citations

Dietary hydroxy fatty acids are absorbed in humans: implications for the measurement of ‘oxidative stress’ in vivo

2002 • 78 citations

Gas chromatographic–mass spectrometric analysis of stable isotopes of cysteine and glutathione in biological samples

1999 • 77 citations

Determination of isoprostanes in urine samples from Alzheimer patients using porous graphitic carbon liquid chromatography–tandem mass spectrometry

2003 • 75 citations

ReviewBiomarkers in Breath Condensate: A promising New Non-invasive Technique in Free Radical Research

2003 • 70 citations

National Heart, Lung, and Blood Institute Clinical Proteomics Working Group Report

2004 • 68 citations

Protein carbonyl groups’ content as a useful clinical marker of antioxidant barrier impairment in plasma of children with juvenile chronic arthritis

2000 • 67 citations

Elevated protein carbonyls as plasma markers of oxidative stress in acute pancreatitis

2003 • 66 citations

Adaptation of the Griess Reaction for Detection of Nitrite in Human Plasma

2004 • 66 citations

Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease

2003 • 60 citations

Acrolein Modifies Apolipoprotein A‐I in the Human Artery Wall

2005 • 56 citations

Specific and rapid quantification of 8-iso-prostaglandin F2α in urine of healthy humans and patients with Zellweger syndrome by gas chromatography–tandem mass spectrometry

1998 • 55 citations

Determination of 3-nitrotyrosine in human urine at the basal state by gas chromatography–tandem mass spectrometry and evaluation of the excretion after oral intake

2005 • 55 citations

NADPH Oxidase of Neutrophils Elevates o,o′-Dityrosine Cross-Links in Proteins and Urine during Inflammation

2001 • 54 citations

Proteomics for the identification of specifically oxidized proteins in brain: Technology and application to the study of neurodegenerative disorders

2003 • 52 citations

Relationship between plasma malondialdehyde levels and adenosine deaminase activities in preeclampsia

2002 • 51 citations

A derivatization assay using gaschromatography/negative chemical ionization tandem mass spectrometry to quantify 3‐nitrotyrosine in human plasma

2003 • 46 citations

Elevated levels of protein carbonyls in sera of chronic fatigue syndrome patients.

2003 • 46 citations

Nitration of endogenous para-hydroxyphenylacetic acid and the metabolism of nitrotyrosine

2003 • 45 citations

Cerebral Vasculature Is the Major Target of Oxidative Protein Alterations in Bacterial Meningitis

2002 • 43 citations

Accurate quantification of basal plasma levels of 3-nitrotyrosine and 3-nitrotyrosinoalbumin by gas chromatography–tandem mass spectrometry

2002 • 43 citations

Specific Analysis in Plasma and Urine of 2,3-Dinor-5,6-dihydro-isoprostane F2α-III, a Metabolite of Isoprostane F2α-III and an Oxidation Product of γ-Linolenic Acid

2000 • 41 citations

The identification of a reaction site of glutathione mixed-disulphide formation on gammaS-crystallin in human lens

2004 • 37 citations

Generation of 8‐epi‐prostaglandin F_2α in isolated rat kidney glomeruli by a radical‐independent mechanism

2001 • 32 citations

Sensitive and accurate analyses of free 3-nitrotyrosine in exhaled breath condensate by LC–MS/MS

2005 • 31 citations

Circulating levels of nitrated apolipoprotein A-I are increased in type 2 diabetic patients

2005 • 24 citations

Quantification of urinary o,o′-dityrosine, a biomarker for oxidative damage to proteins, by high performance liquid chromatography with triple quadrupole tandem mass spectrometryA comparison with ion-trap tandem mass spectrometry

2005 • 24 citations

Plasma 3-NITROTYROSINE and outcome in neonates with severe bronchopulmonary dysplasia after inhaled nitric oxide

2003 • 24 citations

Mass spectrometry in aging research

2004 • 23 citations

Interference of Plasmatic Reduced Glutathione and Hemolysis on Glutathione Disulfide Levels in Human Blood

2004 • 22 citations

Nitric oxide in coronary artery disease: effects of antioxidants

2005 • 15 citations

Cardioprotective effects of carvedilol on acute autoimmune myocarditis

2004 • 15 citations

Is There an Answer?

2005 • 13 citations

Bio-assays for oxidative stress status (BOSS)

2001 • 10 citations

INTRODUCTION TO OXIDATIVE STRESS STATUS (OSS)

2001 • 2 citations