Proteasome: from structure to function

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

Inhibition of Proteasome Activities and Subunit-Specific Amino-Terminal Threonine Modification by Lactacystin

1995 • 1,601 citations

Crystal Structure of the 20 S Proteasome from the Archaeon T. acidophilum at 3.4 Å Resolution

1995 • 1,513 citations

THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATION

1992 • 1,402 citations

Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway.

1995 • 1,296 citations

Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

1995 • 960 citations

Ubiquitin, proteasomes, and the regulation of intracellular protein degradation

1995 • 862 citations

The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.

1995 • 745 citations

A protein catalytic framework with an N-terminal nucleophile is capable of self-activation

1995 • 609 citations

Proteolysis, proteasomes and antigen presentation

1992 • 576 citations

Penicillin acylase has a single-amino-acid catalytic centre

1995 • 448 citations

GENERATION, TRANSLOCATION, AND PRESENTATION OF MHC CLASS I-RESTRICTED PEPTIDES

1995 • 428 citations

Mechanistic aspects of NF-κB regulation: The emerging role of phosphorylation and proteolysis

1995 • 392 citations

Proteasomes: destruction as a programme

1996 • 373 citations

Mechanistic Studies on the Inactivation of the Proteasome by Lactacystin

1996 • 370 citations

Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids

1993 • 323 citations

Protein Degradation or Regulation: Ub the Judge

1996 • 275 citations

Selective protein degradation: A journey's end within the proteasome

1995 • 259 citations

Structural Features of the 26 S Proteasome Complex

1993 • 255 citations

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

1992 • 235 citations

Structure of the Allosteric Regulatory Enzyme of Purine Biosynthesis

1994 • 235 citations

A beta-lactone related to lactacystin induces neurite outgrowth in a neuroblastoma cell line and inhibits cell cycle progression in an osteosarcoma cell line.

1994 • 225 citations

Novel Multiubiquitin Chain Linkages Catalyzed by the Conjugating Enzymes E2EPF and RAD6 Are Recognized by 26 S Proteasome Subunit 5

1996 • 221 citations

Conformational constraints in protein degradation by the 20S proteasome

1995 • 202 citations

Identification of MECL-1 (LMP-10) as the third IFN-gamma-inducible proteasome subunit.

1996 • 196 citations

Primary structures of two homologous subunits of PA28, a γ‐interferon‐inducible protein activator of the 20S proteasome

1995 • 196 citations

The first characterization of a eubacterial proteasome: the 20S complex of Rhodococcus

1995 • 186 citations

Subunit stoichiometry and three-dimensional arrangement in proteasomes from Thermoplasma acidophilum.

1992 • 180 citations

Existence of a molecular ruler in proteasomes suggested by analysis of degradation products

1994 • 179 citations

Three-dimensional structure of human lysosomal aspartylglucosaminidase

1995 • 177 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 172 citations

Degradation of the Proto-Oncogene Product c-Fos by the Ubiquitin Proteolytic System In Vivo and In Vitro: Identification and Characterization of the Conjugating Enzymes

1995 • 167 citations

Incorporation of major histocompatibility complex – encoded subunits LMP2 and LMP7 changes the quality of the 20S proteasome polypeptide processing products independent of interferon‐γ

1995 • 167 citations

Identification of the gal4 suppressor Sug1 as a subunit of the yeast 26S proteasome

1996 • 165 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 160 citations

Proteolysis: The proteasome: a protein-degrading organelle?

1995 • 155 citations

Ubiquitinylation Is Not an Absolute Requirement for Degradation of c-Jun Protein by the 26 S Proteasome

1995 • 150 citations

Arabidopsis MBP1 gene encodes a conserved ubiquitin recognition component of the 26S proteasome.

1996 • 143 citations

Degradation of c-Fos by the 26S Proteasome Is Accelerated by c-Jun and Multiple Protein Kinases

1995 • 140 citations

Kinetic Characterization of the Chymotryptic Activity of the 20S Proteasome

1996 • 137 citations

New insights into proteasome function: from archaebacteria to drug development

1995 • 136 citations

Possible mechanism of nuclear translocation of proteasomes

1990 • 132 citations

Subunits of the regulatory complex of the 26S protease

1995 • 127 citations

Ubiquitin C-terminal hydrolase activity associated with the 26 S protease complex.

1993 • 125 citations

Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1991 • 123 citations

PRE5 and PRE6, the Last Missing Genes Encoding 20S Proteasome Subunits from Yeast? Indication for a Set of 14 Different Subunits in the Eukaryotic Proteasome Core

1994 • 121 citations

Release of a macromolecular protein component from human erythrocyte ghosts

1968 • 117 citations

Structural Features of 26S and 20S Proteasomes

1993 • 107 citations

Degradation of oxidized insulin B chain by the multiproteinase complex macropain (proteasome)

1991 • 99 citations

Evidence Indicating that the Human Proteasome is a Complex Dimer

1993 • 97 citations

LMP2+ proteasomes are required for the presentation of specific antigens to cytotoxic T lymphocytes

1995 • 97 citations

Effects of Major‐Histocompatibility‐Complex‐Encoded Subunits on the Peptidase and Proteolytic Activities of Human 20S Proteasomes

1996 • 95 citations

Human proteasomes analysed with monoclonal antibodies

1995 • 91 citations

Pathways for the processing and presentation of antigens to T cells

1995 • 89 citations

Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli

1992 • 89 citations

Nuclear localization signals of human and Thermoplasma proteasomal alpha subunits are functional in vitro.

1995 • 88 citations

Biochemical properties of the proteasome from Thermoplasma acidophilum

1992 • 78 citations

A Proteasome from the Methanogenic Archaeon Methanosarcina thermophila

1995 • 70 citations

Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease

1996 • 68 citations

Proteasome sequences in eubacteria

1994 • 67 citations

20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences

1996 • 66 citations

Roles of proteasomes in cell growth

1995 • 65 citations

cDNA cloning of a new putative ATPase subunit p45 of the human 26S proteasome, a homolog of yeast transcriptional factor Sug1p

1995 • 60 citations

Thermoplasma acidophilum proteasomes degrade partially unfolded and ubiquitin‐associated proteins

1993 • 52 citations

Appearance of apparently ubiquitin-conjugated lκB-α during its phosphorylation-induced degradation in intact cells

1995 • 51 citations

The proteasome from Thermoplasma acidophilum is neither a cysteine nor a serine protease

1995 • 51 citations

The human proteasome subunit HsN3 is located inthe inner rings of the complex dimer

1995 • 50 citations

Proteasome and class I antigen processing and presentation

1995 • 42 citations

Proteasomes: Multisubunit Proteinases common to Thermoplasma and Eukaryotes

1993 • 40 citations

Catalytic Mechanism of the 20S Proteasome of Thermoplasma acidophilum Revealed by X-ray Crystallography

1995 • 37 citations

Preliminary X-ray Crystallographic Study of the Proteasome from Thermoplasma acidophilum

1993 • 27 citations

A sting in the (N-terminal) tail

1995 • 24 citations

Ordered Structure of the Crystallized Bovine 20S Proteasome1

1995 • 13 citations

Localization of a sequence motif complementary to the nuclear localization signal in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1992 • 9 citations

Peptide traffic across the ER membrane: TAPs and other conduits

1994 • 9 citations