Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

CLUSTAL: a package for performing multiple sequence alignment on a microcomputer

1988 • 3,446 citations

Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.

1984 • 1,467 citations

A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex

1991 • 1,112 citations

Strategies for epitope analysis using peptide synthesis

1987 • 887 citations

The molecular evolution of genes and proteins: a tale of two serines

1988 • 548 citations

The multicatalytic proteinase complex, a major extralysosomal proteolytic system

1990 • 464 citations

A proteasome-related gene between the two ABC transporter loci in the class II region of the human MHC

1991 • 427 citations

Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.

1991 • 421 citations

Identity of the 19S 'prosome' particle with the large multifunctional protease complex of mammalian cells (the proteasome)

1988 • 404 citations

ATP-dependent incorporation of 20S protease into the 26S complex that degrades proteins conjugated to ubiquitin.

1989 • 370 citations

Second proteasome-related gene in the human MHC class II region

1991 • 348 citations

The proteasome (multicatalytic protease) is a component of the 1500-kDa proteolytic complex which degrades ubiquitin-conjugated proteins.

1990 • 327 citations

A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.

1988 • 319 citations

The multicatalytic proteinase (prosome) is ubiquitous from eukaryotes to archaebacteria

1989 • 277 citations

Homology of proteasome subunits to a major histocompatibility complex-linked LMP gene

1991 • 256 citations

The multicatalytic proteinase of mammalian cells

1989 • 242 citations

A sensitive procedure to compare amino acid sequences

1987 • 191 citations

The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.

1984 • 180 citations

Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase

1988 • 179 citations

Involvement of the proteasome in various degradative processes in mammalian cells.

1989 • 162 citations

Electron microscopy and image analysis of the multicatalytic proteinase

1988 • 150 citations

Nuclear location signal-mediated protein transport

1989 • 144 citations

Possible mechanism of nuclear translocation of proteasomes

1990 • 132 citations

Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

1988 • 132 citations

Proteasomes are essential for yeast proliferation. cDNA cloning and gene disruption of two major subunits.

1990 • 127 citations

Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1991 • 123 citations

Release of a macromolecular protein component from human erythrocyte ghosts

1968 • 117 citations

Molecular cloning and functional analysis of three subunits of yeast proteasome.

1991 • 97 citations

The three‐dimensional structure of proteasomes from Thermoplasma acidophilum as determined by electron microscopy using random conical tilting

1991 • 92 citations

The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.

1989 • 89 citations

Functional evolutionary divergence of proteolytic enzymes and their inhibitors

1989 • 89 citations

Identification of a fourth class of proteins linked to the murine major histocompatibility complex.

1982 • 87 citations

Molecular cloning of cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver: primary structure of the largest component (C2)

1989 • 86 citations

Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes)

1991 • 81 citations

H–2-linked low-molecular weight polypeptide antigens assemble into an unusual macromolecular complex

1984 • 80 citations

Cloning and sequencing of the gene encoding the large (α‐) subunit of the proteasome from Thermoplasma acidophilum

1991 • 80 citations

Molecular cloning of cDNA for proteasomes from rat liver: primary structure of component C3 with a possible tyrosine phosphorylation site

1990 • 73 citations

Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (Multiprotease complexes) in liver

1989 • 69 citations

Relationships among the subunits of the high molecular weight proteinase, macropain (proteasome)

1990 • 67 citations

cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells

1990 • 67 citations

Prosomes, subcomplexes of untranslated mRNP

1990 • 65 citations

The primary structures of four subunits of the human, high-molecular-weight proteinase, macropain (proteasome), are distinct but homologous

1991 • 56 citations

N‐Terminal sequence similarities between components of the multicatalytic proteinase complex

1990 • 56 citations

The Drosophila PROS-28.1 gene is a member of the proteasome gene family

1990 • 54 citations

cDNA cloning and sequencing of component C8 of proteasomes from rat hepatoma cells

1990 • 50 citations

cDNA cloning and sequencing of component C5 of proteasomes from rat hepatoma cells

1990 • 48 citations

Electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groEL-Type proteins and proteasomes

1990 • 46 citations

The structure and organization of the 16S ribosomal RNA gene from the archaebacterium Thermoplasma acidophilum

1989 • 36 citations

Nucleotide sequence ofPUP1encoding a putative proteasome subunit inSaccharomyces cerevisiae

1991 • 33 citations

The Drosophila PROS-29 gene is a new member of the PROS-gene family

1990 • 32 citations

Deduced primary structure of a Xenopus proteasome subunit XC3 and expression of its mRNA during early development

1991 • 20 citations

Cloning and sequence analysis of pituitary cDNA encoding the β‐subunit of Xenopus proteasome

1991 • 17 citations