Degradation of the Proto-Oncogene Product c-Fos by the Ubiquitin Proteolytic System In Vivo and In Vitro: Identification and Characterization of the Conjugating Enzymes

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53

1990 • 3,896 citations

The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation

1991 • 3,396 citations

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53

1993 • 2,329 citations

The ubiquitinproteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB

1994 • 2,124 citations

Role of the Ubiquitin-Proteasome Pathway in Regulating Abundance of the Cyclin-Dependent Kinase Inhibitor p27

1995 • 1,848 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

Fos and jun: The AP-1 connection

1988 • 1,597 citations

Control of IκB-α Proteolysis by Site-Specific, Signal-Induced Phosphorylation

1995 • 1,443 citations

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1983 • 1,105 citations

Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin A

1991 • 1,054 citations

Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

1995 • 960 citations

Bone and haematopoietic defects in mice lacking c-fos

1992 • 939 citations

Ubiquitin-dependent c-Jun degradation in vivo is mediated by the δ domain

1994 • 930 citations

Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination

1992 • 780 citations

The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme

1987 • 731 citations

A proteasome inhibitor prevents activation of NF-kappa B and stabilizes a newly phosphorylated form of I kappa B-alpha that is still bound to NF-kappa B.

1994 • 723 citations

FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA

1982 • 668 citations

Pleiotropic effects of a null mutation in the c-fos proto-oncogene

1992 • 643 citations

THE UBIQUITIN-CONJUGATION SYSTEM

1992 • 561 citations

Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.

1993 • 537 citations

Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini

1983 • 532 citations

Viral and cellular fos proteins: A comparative analysis

1984 • 523 citations

Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.

1990 • 520 citations

Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation.

1986 • 468 citations

Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.

1983 • 446 citations

Avian sarcoma virus 17 carries the jun oncogene.

1987 • 445 citations

Ubiquitination

1991 • 430 citations

Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation.

1982 • 413 citations

Electric field mediated gene transfer

1982 • 364 citations

Degradation of nuclear oncoproteins by the ubiquitin system in vitro.

1991 • 345 citations

A role for the ubiquitin-dependent proteolytic pathway in MHC class l-restricted antigen presentation

1993 • 317 citations

Cyclin Ubiquitination: The destructive end of mitosis

1995 • 304 citations

The leucine repeat motif in Fos protein mediates complex formation with Jun/AP-1 and is required for transformation

1989 • 286 citations

Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway.

1994 • 274 citations

Microinjection of fos-specific antibodies blocks DNA synthesis in fibroblast cells.

1988 • 266 citations

Occurrence of a polyubiquitin structure in ubiquitin-protein conjugates

1985 • 263 citations

Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53.

1994 • 259 citations

Methylated ubiquitin inhibits cyclin degradation in clam embryo extracts.

1991 • 246 citations

The short-lived MAT alpha 2 transcriptional regulator is ubiquitinated in vivo.

1991 • 242 citations

Ubiquitin-aldehyde: a general inhibitor of ubiquitin-recycling processes.

1987 • 189 citations

A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.

1988 • 173 citations

Degradation of transcription factors, c‐Jun and c‐Fos, by calpain

1991 • 163 citations

Ubiquitinylation Is Not an Absolute Requirement for Degradation of c-Jun Protein by the 26 S Proteasome

1995 • 150 citations

The ‘second-codon rule’ and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.

1992 • 147 citations

Reconstitution of p53-ubiquitinylation reactions from purified components: the role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP).

1995 • 117 citations

Targeted Degradation of c-Fos, But Not v-Fos, by a Phosphorylation-Dependent Signal on c-Jun

1992 • 110 citations

The ubiquitin-activating enzyme, E1, is required for stress-induced lysosomal degradation of cellular proteins

1991 • 101 citations

Degradation of the tumor suppressor protein p53 by the ubiquitin-mediated proteolytic system requires a novel species of ubiquitin-carrier protein, E2

1994 • 100 citations

Drosophila UbcD1 encodes a highly conserved ubiquitin-conjugating enzyme involved in selective protein degradation.

1992 • 75 citations

Affinity purification of ubiquitin-protein ligase on immobilized protein substrates. Evidence for the existence of separate NH2-terminal binding sites on a single enzyme.

1990 • 73 citations

Differential sensitivity of FOS and JUN family members to calpains.

1994 • 69 citations

Purification and characterization of a novel species of ubiquitin-carrier protein, E2, that is involved in degradation of non-“N-end rule” protein substrates

1994 • 66 citations

A ubiquitin-protein ligase specific for type III protein substrates.

1990 • 62 citations

Altered I-A protein-mediated transmembrane signaling in B cells that express truncated I-Ak protein.

1989 • 61 citations

Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana

1993 • 48 citations

Determining the effect of inducible protein phosphorylation on the DNA-binding activity of transcription factors

1992 • 47 citations

Uncoupling ubiquitin-protein conjugation from ubiquitin-dependent proteolysis by use of .beta.,.gamma.-nonhydrolyzable ATP analogs

1991 • 43 citations

A major ubiquitin conjugation system in wheat germ extracts involves a 15-kDa ubiquitin-conjugating enzyme (E2) homologous to the yeast UBC4/UBC5 gene products.

1993 • 42 citations

Complete reconstitution of conjugation and subsequent degradation of the tumor suppressor protein p53 by purified components of the ubiquitin proteolytic system

1994 • 39 citations

Purification and characterization of a novel protein that is required for degradation of N-alpha-acetylated proteins by the ubiquitin system.

1991 • 31 citations

Rescue of the complex temperature-sensitive phenotype of Chinese hamster ovary E36ts20 cells by expression of the human ubiquitin-activating enzyme cDNA

1994 • 29 citations