Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Proteolysis, proteasomes and antigen presentation

1992 • 576 citations

The N-End Rule in Bacteria

1991 • 536 citations

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

1992 • 235 citations

Subunit stoichiometry and three-dimensional arrangement in proteasomes from Thermoplasma acidophilum.

1992 • 180 citations

Electron microscopy and image analysis of the multicatalytic proteinase

1988 • 150 citations

In vivo function of the proteasome in the ubiquitin pathway.

1992 • 144 citations

Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

1988 • 132 citations

The Drosophila proteasome undergoes changes in its subunit pattern during development

1989 • 127 citations

Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1991 • 123 citations

The three‐dimensional structure of proteasomes from Thermoplasma acidophilum as determined by electron microscopy using random conical tilting

1991 • 92 citations

Cloning and sequencing of the gene encoding the large (α‐) subunit of the proteasome from Thermoplasma acidophilum

1991 • 80 citations

An efficient method to purify active eukaryotic proteins from the inclusion bodies in Escherichia coli.

1991 • 78 citations

The proteasome/multicatalytic—multifunctional proteinase In vivo function in the ubiquitin‐dependent N‐end rule pathway of protein degradation in eukaryotes

1992 • 76 citations

A 20S particle ubiquitous from yeast to human

1987 • 67 citations

Phosphorylation of the multicatalytic proteinase complex from bovine pituitaries by a copurifying cAMP-dependent protein kinase

1990 • 60 citations

Citrate synthase from the thermophilic archaebacterium Thermoplasma acidophilum

1990 • 53 citations

Electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groEL-Type proteins and proteasomes

1990 • 46 citations

Overexpression of the methanococcal ribosomal protein L12 in Escherichia coli and its incorporation into halobacterial 50 S subunits yielding active ribosomes.

1990 • 21 citations

Expression and purification of plasmid‐encoded Thermoplasma acidophilum citrate synthase from Escherichia coli

1991 • 19 citations

Genetic engineering and overexpression of ribosomal L12 protein genes from three different archaebacteria in E coli

1991 • 2 citations