Altered regulation of platelet-derived growth factor A-chain and c-fos gene expression in senescent progeria fibroblasts

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949 • 19,979 citations

LIGAND: A versatile computerized approach for characterization of ligand-binding systems

1980 • 8,534 citations

The serial cultivation of human diploid cell strains

1961 • 7,886 citations

The limited in vitro lifetime of human diploid cell strains

1965 • 5,595 citations

Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins

1989 • 3,219 citations

The biology of platelet-derived growth factor

1986 • 2,036 citations

Phospholipase C-γ is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro

1989 • 978 citations

cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines

1986 • 786 citations

Signal Transduction by the Platelet-Derived Growth Factor Receptor

1989 • 653 citations

Interleukin-1 Mitogenic Activity for Fibroblasts and Smooth Muscle Cells Is Due to PDGF-AA

1989 • 648 citations

Two Different Subunits Associate to Create Isoform-Specific Platelet-derived Growth Factor Receptors

1989 • 633 citations

Isolation of a Novel Receptor cDNA Establishes the Existence of Two PDGF Receptor Genes

1989 • 610 citations

Two Classes of PDGF Receptor Eecognize Different Isoforms of PDGF

1988 • 603 citations

c-fos protein can induce cellular transformation: A novel mechanism of activation of a cellular oncogene

1984 • 601 citations

PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex

1990 • 561 citations

Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins

1989 • 524 citations

Binding of different dimeric forms of PDGF to human fibroblasts: evidence for two separate receptor types.

1988 • 514 citations

Direct activation of the serine/threonine kinase activity of raf-1 through tyrosine phosphorylation by the PDGF β-receptor

1989 • 513 citations

Repression of c- fos Transcription and an Altered Genetic Program in Senescent Human Fibroblasts

1990 • 478 citations

Superinduction of c- fos by Nerve Growth Factor in the Presence of Peripherally Active Benzodiazepines

1985 • 478 citations

Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation.

1986 • 468 citations

A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains

1986 • 418 citations

Mutator phenotype of Werner syndrome is characterized by extensive deletions.

1989 • 417 citations

cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.

1989 • 413 citations

The Hutchinson-Gilford progeria syndrome

1972 • 389 citations

Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells.

1987 • 381 citations

cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules.

1988 • 338 citations

A major part of platelet-derived growth factor purified from human platelets is a heterodimer of one A and one B chain.

1988 • 317 citations

Cloning and expression of a cDNA coding for the human platelet-derived growth factor receptor: evidence for more than one receptor class.

1988 • 314 citations

Microinjection of fos-specific antibodies blocks DNA synthesis in fibroblast cells.

1988 • 266 citations

Synthesis and secretion of platelet-derived growth factor by human breast cancer cell lines.

1987 • 266 citations

Purification and properties of porcine platelet-derived growth factor.

1984 • 260 citations

Characterization of a tyrosine-specific kinase activity in human fibroblast membranes stimulated by platelet-derived growth factor.

1982 • 253 citations

Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts.

1986 • 247 citations

Cytogenetics of Werner’s syndrome cultured skin fibroblasts: variegated translocation mosaicism

1981 • 241 citations

Interaction of platelet-derived growth factor with its fibroblast receptor. Demonstration of ligand degradation and receptor modulation.

1982 • 239 citations

Replicative senescence of human skin fibroblasts correlates with a loss of regulation and overexpression of collagenase activity

1989 • 237 citations

Possible positive autocrine feedback in the prereplicative phase of human fibroblasts

1987 • 234 citations

Sera and conditioned media contain different isoforms of platelet-derived growth factor (PDGF) which bind to different classes of PDGF receptor

1989 • 216 citations

Expression and developmental control of platelet-derived growth factor A-chain and B-chain/Sis genes in rat aortic smooth muscle cells.

1988 • 214 citations

Evidence for the platelet-derived growth factor-stimulated tyrosine phosphorylation of the platelet-derived growth factor receptor in vivo. Immunopurification using a monoclonal antibody to phosphotyrosine.

1984 • 209 citations

Arterial smooth muscle cells express platelet-derived growth factor (PDGF) A chain mRNA, secrete a PDGF-like mitogen, and bind exogenous PDGF in a phenotype- and growth state-dependent manner

1988 • 205 citations

Defining cellular senescence in IMR-90 cells: a flow cytometric analysis.

1988 • 196 citations

Existence of High Abundance Antiproliferative mRNA's in Senescent Human Diploid Fibroblasts

1986 • 194 citations

Human vascular smooth muscle cells both express and respond to heparin-binding growth factor I (endothelial cell growth factor).

1987 • 194 citations

Isoform-specific induction of actin reorganization by platelet-derived growth factor suggests that the functionally active receptor is a dimer.

1989 • 191 citations

Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

1988 • 187 citations

Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor (PDGF) and produce a PDGF-like growth factor.

1986 • 187 citations

Identification and Structural Analysis of the A Type Receptor for Platelet-derived Growth Factor

1989 • 179 citations

Cellular Senescence revisited: a review

1987 • 171 citations

Use of an antiserum against phosphotyrosine for the identification of phosphorylated components in human fibroblasts stimulated by platelet-derived growth factor.

1984 • 163 citations

Rat skeletal myoblasts and arterial smooth muscle cells express the gene for the A chain but not the gene for the B chain (c-sis) of platelet-derived growth factor (PDGF) and produce a PDGF-like protein.

1986 • 162 citations

Multiple forms of endothelial cell growth factor. Rapid isolation and biological and chemical characterization.

1985 • 160 citations

Dominance of the Senescent Phenotype in Heterokaryons Between Replicative and Post-Replicative Human Fibroblast-Like Cells

1974 • 157 citations

TGF‐β stimulates primary human skin fibroblast DNA synthesis via an autocrine production of PDGF‐related peptides

1989 • 154 citations

Serum-Inducible Genes

1989 • 143 citations

Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.

1988 • 139 citations

Independent expression of human alpha or beta platelet-derived growth factor receptor cDNAs in a naive hematopoietic cell leads to functional coupling with mitogenic and chemotactic signaling pathways.

1989 • 137 citations

Regulated expression of the platelet-derived growth factor A chain gene in microvascular endothelial cells.

1987 • 126 citations

A 57,000-mol-wt protein uniquely present in nonproliferating cells and senescent human fibroblasts.

1985 • 125 citations

Cultured human endothelial cells express platelet-derived growth factor A chain.

1987 • 115 citations

Progressive Loss of the Proliferative Response of Senescing Wi-38 Cells to Platelet-derived Growth Factor, Epidermal Growth Factor, Insulin, Transferrin, and Dexamethasone

1984 • 113 citations

Systematic growth studies, cocultivation, and cell hybridization studies of Werner syndrome cultured skin fibroblasts

1981 • 109 citations

Progeria: a cell culture study on aging

1971 • 100 citations

Growth Factor Responsiveness of Cultured Human Fibroblasts Declines with Age

1983 • 99 citations

A human glioma cell line secretes three structurally and functionally different dimeric forms of platelet‐derived growth factor

1988 • 88 citations

Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro*1

1984 • 87 citations

Heparin-binding growth factor 1 stimulates tyrosine phosphorylation in NIH 3T3 cells.

1989 • 85 citations

[125I]EGF binding ability is stable throughout the replicative life-span of WI-38 cells

1983 • 84 citations

Diminished Response of Werner's Syndrome Fibroblasts to Growth Factors PDGF and FGF

1986 • 79 citations

Senescent and quiescent cell inhibitors of DNA synthesis

1985 • 79 citations

Alterations in the Responsiveness of Senescent Cells to Growth Factors

1989 • 79 citations

Both homodimeric isoforms of PDGF (AA and BB) have mitogenic and chemotactic activity and stimulate phosphoinositol turnover

1989 • 78 citations

Purification of the platelet-derived growth factor receptor by using an anti-phosphotyrosine antibody.

1985 • 75 citations

Molecular biology of aging

1985 • 73 citations

Regulation of ornithine decarboxylase and other cell cycle-dependent genes during senescence of IMR-90 human diploid fibroblasts.

1988 • 73 citations

Ongoing DNA synthesis continues in young human diploid cells (HDC) fused to senescent HDC, but entry into S phase is inhibited

1980 • 73 citations

Platelet-derived growth factor receptors expressed by cDNA transfection couple to a diverse group of cellular responses associated with cell proliferation.

1988 • 73 citations

Expression of recombinant platelet-derived growth factor A- and B-chain homodimers in rat-1 cells and human fibroblasts reveals differences in protein processing and autocrine effects.

1988 • 71 citations

Insulin‐like growth factor‐I: Specific binding to high and low affinity sites and mitogenic action throughout the life span of WI‐38 cells

1987 • 67 citations

Syndromes of Premature Aging

1987 • 66 citations

Heparin-binding growth factor-1 stimulation of human endothelial cells induces platelet-derived growth factor A-chain gene expression.

1990 • 65 citations

Purified human platelet-derived growth factor receptor has ligand-stimulated tyrosine kinase activity.

1986 • 64 citations

Human Genetic Disorders That Feature Premature Onset and Accelerated Progression of Biological Aging

1978 • 62 citations

c-Ha-ras-1 proto-oncogene amplification and overexpression during the limited replicative life span of normal human fibroblasts.

1985 • 60 citations

Alternative splicing of the platelet-derived growth factor A-chain transcript occurs in normal as well as tumor cells and is conserved among mammalian species.

1989 • 58 citations

Cell enlargement: One possible mechanism underlying cellular senescence

1989 • 58 citations

Growth factors induce early pre-replicative changes in senescent human fibroblasts.

1986 • 57 citations

Deletion of the kinase insert sequence of the platelet-derived growth factor beta-receptor affects receptor kinase activity and signal transduction.

1990 • 55 citations

Density-Dependent Inhibition of Cell Growth by Transforming Growth Factor-β1 in Normal Human Fibroblasts

1988 • 51 citations

Inhibition of DNA synthesis in proliferating human diploid fibroblasts by fusion with senescent cytoplasts*1

1983 • 49 citations

Decreased sensitivity of old and progeric human fibroblasts to a preparation of factors with insulinlike activity.

1981 • 48 citations

Inhbition of DNA synthesis in young cycling human diploid fibroblast-like cells upon fusion to enucleate cytoplasts from senescent cells

1983 • 44 citations

Human iliac artery endothelial cells express both genes encoding the chains of platelet‐derived growth factor (PDGF) and synthesize PDGF‐like mitogen

1987 • 42 citations

Tumor necrosis factor-induced expression of platelet-derived growth factor A-chain messenger RNA in fibroblasts

1989 • 42 citations

Regulation of c-myc and c-fos proto-oncogene expression by animal cell growth factors

1988 • 41 citations

EGF‐dependent phosphorylation of the EGF receptor in plasma membranes isolated from young and senescent WI‐38 cells

1987 • 36 citations

Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts

1987 • 34 citations

Identification of a highly abundant cDNA isolated from senescent WI-38 cells

1989 • 29 citations

Loss of responsiveness in senescent human TIG-1 cells to the DNA synthesis-inducing effect of various growth factors

1984 • 28 citations

Patterns of peptide synthesis in senescent and presenescent human fibroblasts

1979 • 27 citations

Cultured primate aortic smooth muscle cells express both the PDGF‐‐A and PDGF‐B genes but do not secrete mitogenic activity or dimeric platelet‐derived growth factor protein

1988 • 24 citations

Spreading behavior and surface characteristics of young and senescent WI38 fibroblasts revealed by scanning electron microscopy

1979 • 23 citations

The Two-dimensional polypeptide profile of terminally non-dividing human diploid cells*1

1984 • 21 citations

Enhanced synthesis of a Mr = 55,000 dalton peptide by senescent human fibroblasts

1987 • 20 citations

A B-type PDGF receptor lacking most of the intracellular domain escapes degradation after ligand binding

1989 • 20 citations

Two-Dimensional Electrophoretic Analysis of Nuclear Acidic Proteins in Senescent Human Diploid Cells

1979 • 17 citations

Absence of three secreted proteins and presence of a 57-kDa protein related to irreversible arrest of cell growth.

1988 • 14 citations

Styl RFLP of the human platelet derived growth factor (PDGF) A-chain gene

1990 • 5 citations