Analysis of apoptosis by laser scanning cytometry

Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics

1972 • 15,349 citations

Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.

1992 • 9,540 citations

Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade

1997 • 7,177 citations

Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked

1997 • 4,921 citations

The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis

1997 • 4,728 citations

A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry

1991 • 4,682 citations

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis

1995 • 4,003 citations

Apoptosis, oncosis, and necrosis. An overview of cell death.

1995 • 3,325 citations

Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages.

1992 • 3,279 citations

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE

1994 • 2,575 citations

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis

1994 • 2,450 citations

Bcl-2 and the regulation of programmed cell death

1994 • 2,346 citations

Bad, a heterodimeric partner for Bcl-xL and Bcl-2, displaces bax and promotes cell death

1995 • 2,114 citations

Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis

1994 • 1,969 citations

Features of apoptotic cells measured by flow cytometry

1992 • 1,912 citations

Annexin V-Affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure

1998 • 1,850 citations

Movement of Bax from the Cytosol to Mitochondria during Apoptosis

1997 • 1,805 citations

Programmed cell death involved in the schizolysigenous formation of the secretory cavity in Citrus sinensis L. (Osbeck)

2010 • 1,629 citations

Localization of mitochondria in living cells with rhodamine 123.

1980 • 1,529 citations

Bax directly induces release of cytochrome c from isolated mitochondria

1998 • 1,514 citations

Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.

1991 • 1,505 citations

X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death

1996 • 1,456 citations

Mitochondrial control of nuclear apoptosis.

1996 • 1,363 citations

Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

1966 • 1,150 citations

Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis)

1997 • 1,082 citations

Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays.

1993 • 997 citations

Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease

1994 • 981 citations

Apoptosis

1993 • 972 citations

Programmed Death of T Cells in HIV-1 Infection

1992 • 932 citations

Manual of Clinical Laboratory Immunology

1997 • 833 citations

A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA.

1993 • 823 citations

Checkpoints of dueling dimers foil death wishes

1994 • 806 citations

Poly(ADP-ribose) polymerase: a molecular nick-sensor

1994 • 790 citations

Flow cytometry of apoptotic cell death

2000 • 764 citations

Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques.

1994 • 697 citations

Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation

1992 • 685 citations

A Selective Procedure for DNA Extraction from Apoptotic Cells Applicable for Gel Electrophoresis and Flow Cytometry

1994 • 670 citations

Signal Transduction by DR3, a Death Domain-Containing Receptor Related to TNFR-1 and CD95

1996 • 653 citations

Interaction of CED-4 with CED-3 and CED-9: A Molecular Framework for Cell Death

1997 • 635 citations

Toward an understanding of the molecular mechanisms of physiological cell death.

1993 • 623 citations

Selenium: Relation to Decreased Toxicity of Methylmercury Added to Diets Containing Tuna

1972 • 623 citations

Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis.

1995 • 598 citations

Internucleosomal DNA Cleavage Should not be the Sole Criterion for Identifying Apoptosis

1992 • 462 citations

Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry

1992 • 443 citations

Expression of bcl-xL can confer a multidrug resistance phenotype

1995 • 421 citations

Subcellular and submitochondrial mode of action of Bcl-2-like oncoproteins

1998 • 421 citations

The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents.

1993 • 403 citations

Mch3, a novel human apoptotic cysteine protease highly related to CPP32.

1995 • 391 citations

Apoptosis Induction by Caspase-8 Is Amplified through the Mitochondrial Release of Cytochrome c

1998 • 380 citations

Chapter 2 Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis

1994 • 354 citations

Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis.

1996 • 353 citations

Interaction and Regulation of Subcellular Localization of CED-4 by CED-9

1997 • 345 citations

Laser-Scanning Cytometry: A New Instrumentation with Many Applications

1999 • 310 citations

Bcl-2 blocks loss of mitochondrial membrane potential while ICE inhibitors act at a different step during inhibition of death induced by respiratory chain inhibitors.

1996 • 300 citations

Induction of DNA strand breaks associated with apoptosis during treatment of leukemias.

1993 • 292 citations

Detection of DNA fragmentation in apoptosis: application of in situ nick translation to cell culture systems and tissue sections.

1993 • 276 citations

Evolution of the serologic response to Borrelia burgdorferi in treated patients with culture-confirmed erythema migrans

1996 • 263 citations

Activation of Caspases Measured in Situ by Binding of Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Correlation with DNA Fragmentation

2000 • 257 citations

DNA STRAND BREAKS OCCURRING DURING APOPTOSIS - THEIR EARLY INSITU DETECTION BY THE TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND NICK TRANSLATION ASSAYS AND PREVENTION BY SERINE PROTEASE INHIBITORS

1992 • 253 citations

Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry

1994 • 245 citations

Microscope‐based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data

1991 • 242 citations

Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry

1994 • 226 citations

Delayed internucleosomal DNA fragmentation in programmed cell death

1993 • 222 citations

Interaction of rhodamine 123 with living cells studied by flow cytometry.

1982 • 220 citations

Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis

1995 • 219 citations

Functional Heterogeneity of an Isolated Mitochondrial Population Revealed by Cytofluorometric Analysis at the Single Organelle Level

1996 • 212 citations

Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation

1995 • 211 citations

Apoptosis in interleukin-3-dependent haemopoietic cells

1992 • 207 citations

Increased membrane permeability of apoptotic thymocytes: A flow cytometric study

1993 • 200 citations

Mitochondrial Modifications during Rat Thymocyte Apoptosis: A Study at the Single Cell Level

1994 • 192 citations

Slide-Based Laser Scanning Cytometry

1997 • 188 citations

Single‐step procedure for labeling DNA strand breaks with fluorescein‐ or Bodipy‐conjugated deoxynucleotides: Detection of apoptosis and bromodeoxyuridine incorporation

1995 • 178 citations

Supravital exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentation

1996 • 160 citations

Quantification of apoptotic cells with fluorescein isothiocyanate-labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin

1996 • 158 citations

The thiol crosslinking agent diamide overcomes the apoptosis-inhibitory effect of Bcl-2 by enforcing mitochondrial permeability transition

1998 • 155 citations

In vivo DNA degradation in thymocytes of γ-irradiated or hydrocortisone-treated rats

1981 • 146 citations

Challenges for the development of female-controlled vaginal microbicides

1994 • 143 citations

Demonstration of extensive chromatin cleavage in transplanted Morris hepatoma 7777 tissue: apoptosis or necrosis?

1993 • 141 citations

Apoptosis in anititumor strategies: Modulation of cell cycle or differentiation

1995 • 138 citations

Caspases disrupt mitochondrial membrane barrier function

1998 • 137 citations

The study of apoptotic cells by flow cytometry

1998 • 129 citations

bcl-2 in cancer, development and apoptosis

1994 • 128 citations

Mitochondria alterations and dramatic tendency to undergo apoptosis in peripheral blood lymphocytes during acute HIV syndrome

1997 • 126 citations

Nucleic acid dyes for detection of apoptosis in live cells

1995 • 125 citations

Cleavage of Poly(ADP-Ribose) Polymerase Measured in Situ in Individual Cells: Relationship to DNA Fragmentation and Cell Cycle Position during Apoptosis

2000 • 124 citations

Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA

1994 • 123 citations

Detection of apoptosis in live cells by MitoTracker? Red CMXRos and SYTO dye flow cytometry

1997 • 122 citations

Detection of Apoptosis and DNA Replication by Differential Labeling of DNA Strand Breaks with Fluorochromes of Different Color

1996 • 119 citations

CED-4—The Third Horseman of Apoptosis

1997 • 116 citations

Role of proteolysis in apoptosis: involvement of serine proteases in internucleosomal DNA fragmentation in immature thymocytes

1993 • 109 citations

The apoptosis endonuclease and its regulation.

1992 • 108 citations

Expression of the Bcl-2 homologue Mcl-1 correlates with survival of peripheral blood B lymphocytes.

1996 • 103 citations

Thymus-Derived Lymphocytes: Humoral and Cellular Reactions Distinguished by Hydrocortisone

1972 • 99 citations

Activation of nuclear factor kappa B (NF-?B) assayed by laser scanning cytometry (LSC)

1998 • 97 citations

The mitochondrion as an integrator/coordinator of cell death pathways

1998 • 97 citations

Apoptotic Cell Death During Treatment of Leukemias

1994 • 96 citations

Resolution of mitotic cells using laser scanning cytometry

1996 • 86 citations

Radiation-induced Apoptosis and Necrosis in Molt-4 Cells: A Study of Dose-effect Relationships and Their Modification

1993 • 84 citations

Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry

2000 • 83 citations

Techniques in Apoptosis: A User's Guide.

1997 • 79 citations

Apoptosis of rat thymocytes triggered by prednisolone, camptothecin, or teniposide is selective to G0 cells and is prevented by inhibitors of proteases.

1992 • 75 citations

Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry

2000 • 63 citations

Etoposide-induced cytotoxicity in two human T-cell leukemic lines: delayed loss of membrane permeability rather than DNA fragmentation as an indicator of programmed cell death.

1993 • 62 citations

Laser scanning cytometry distinguishes lymphocytes, monocytes, and granulocytes by differences in their chromatin structure

1997 • 61 citations

Generation of reactive oxygen intermediates after treatment of blasts of acute myeloblastic leukemia with cytosine arabinoside: role of bcl-2.

1996 • 59 citations

Simplified immunophenotypic analysis by laser scanning cytometry

1998 • 56 citations

Effect of Protease Inhibitors on Early Events of Apoptosis

1996 • 54 citations

The Schrödinger's Cat Quandary in Cell Biology: Integration of Live Cell Functional Assays with Measurements of Fixed Cells in Analysis of Apoptosis

1999 • 52 citations

Cell cycle specificity of apoptosis during treatment of leukaemias

1997 • 52 citations

Multiparameter Immunophenotypic Analysis of Fine Needle Aspiration Biopsies and Other Hematologic Specimens by Laser Scanning Cytometry

1997 • 51 citations

Enzyme kinetic reactions and fluorochrome uptake rates measured in individual cells by laser scanning cytometry

1998 • 49 citations

Laser Scanning Cytometry in Pathology of Solid Tumors

1997 • 49 citations

Laser scanning cytometry allows detection of cell death with morphological features of apoptosis in cells stained with PI

1997 • 46 citations

Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive patients by simultaneous flow cytometric analysis of intracellular HIV RNA and cellular immunophenotype

1998 • 45 citations

Five-color immunophenotyping plus DNA content analysis by laser scanning cytometry

1998 • 42 citations

Human Granulocytic Ehrlichiosis: Report of a Case in Northern California

1996 • 42 citations

Laser scanning cytometry (LCS) allows detailed analysis of the cell cycle in PI stained human fibroblasts (TIG-7)

1997 • 38 citations

Evaluation of in vivo induction of apoptosis in patients with acute leukemia treated on a phase I study of paclitaxel.

1995 • 31 citations

Comparative analysis of apoptosis measured by Hoechst and flow cytometry in non-Hodgkin's lymphomas

1998 • 30 citations

Laser scanning cytometer (LSC) analysis of fraction of labelled mitoses (FLM)

1996 • 30 citations

Cell cycle effects and induction of apoptosis caused by infection of HL-60 cells with human granulocytic ehrlichiosis pathogen measured by flow and laser scanning cytometry

1998 • 27 citations

The Bcl-2 family of proteins and activation of the ICE-CED-3 family of proteases: A balancing act in apoptosis?

1997 • 21 citations

Novel approach for simultaneous evaluation of cell phenotype, apoptosis, and cell cycle using multiparameter flow cytometry

1998 • 17 citations

Analysis and sorting of apoptotic cells from fine-needle aspirates of excised human primary breast carcinomas

1998 • 15 citations

Apoptosis and some of its medical implications.

1998 • 13 citations