Cell-free nucleic acids as biomarkers in cancer patients

Global cancer statistics

2011 • 55,498 citations

MicroRNAs: Target Recognition and Regulatory Functions

2009 • 19,026 citations

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

2009 • 14,044 citations

MicroRNA expression profiles classify human cancers

2005 • 9,457 citations

Circulating microRNAs as stable blood-based markers for cancer detection

2008 • 7,573 citations

EGFRMutation and Resistance of Non–Small-Cell Lung Cancer to Gefitinib

2005 • 3,807 citations

Inhibition of Mutated, Activated BRAF in Metastatic Melanoma

2010 • 3,500 citations

Targeting RAS signalling pathways in cancer therapy

2003 • 3,105 citations

Causes and consequences of microRNA dysregulation in cancer

2009 • 2,879 citations

Genomic DNA methylation: the mark and its mediators

2006 • 2,428 citations

Circulating mutant DNA to assess tumor dynamics

2008 • 2,395 citations

Linking DNA methylation and histone modification: patterns and paradigms

2009 • 2,133 citations

DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells.

2001 • 2,050 citations

EGFR Antagonists in Cancer Treatment

2008 • 1,940 citations

Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer

1999 • 1,865 citations

The first 30 years of p53: growing ever more complex

2009 • 1,805 citations

Detection of elevated levels of tumour‐associated microRNAs in serum of patients with diffuse large B‐cell lymphoma

2008 • 1,692 citations

Shedding microvesicles: artefacts no more

2009 • 1,679 citations

Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosis

2010 • 1,293 citations

Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

2009 • 1,120 citations

Detection and quantification of mutations in the plasma of patients with colorectal tumors

2005 • 1,118 citations

Nuclear Acids In Human Blood Plasma.

1948 • 1,106 citations

Detection, clinical relevance and specific biological properties of disseminating tumour cells

2008 • 1,098 citations

A novel and universal method for microRNA RT-qPCR data normalization

2009 • 985 citations

Histone Deacetylase Inhibitors in Cancer Therapy

2009 • 854 citations

Quantification of Plasma Epstein–Barr Virus DNA in Patients with Advanced Nasopharyngeal Carcinoma

2004 • 840 citations

Circulating nucleic acids (CNAs) and cancer—A survey

2006 • 821 citations

Serum MicroRNA Signatures Identified in a Genome-Wide Serum MicroRNA Expression Profiling Predict Survival of Non–Small-Cell Lung Cancer

2010 • 784 citations

Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma.

1999 • 749 citations

Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours

2001 • 747 citations

Circulating tumour cells in cancer patients: challenges and perspectives

2010 • 695 citations

Microsatellite alterations in serum DNA of head and neck cancer patients

1996 • 653 citations

Circulating Methylated SEPT9 DNA in Plasma Is a Biomarker for Colorectal Cancer

2009 • 509 citations

Soluble normal and mutated DNA sequences from single-copy genes in human blood.

1994 • 478 citations

Direct Serum Assay for MicroRNA-21 Concentrations in Early and Advanced Breast Cancer

2010 • 434 citations

Circulating microRNAs as blood-based markers for patients with primary and metastatic breast cancer

2010 • 409 citations

Plasma Epstein-Barr Virus DNA and Residual Disease After Radiotherapy for Undifferentiated Nasopharyngeal Carcinoma

2002 • 409 citations

Point mutations of the N‐ras gene in the blood plasma DNA of patients with myelodysplastic syndrome or acute myelogenous leukaemia

1994 • 392 citations

Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay

2008 • 386 citations

The methyl-CpG binding domain and the evolving role of DNA methylation in animals

2003 • 386 citations

Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients.

2001 • 384 citations

The Origin and Mechanism of Circulating DNA

2000 • 370 citations

Plasma Epstein-Barr Viral Deoxyribonucleic Acid Quantitation Complements Tumor-Node-Metastasis Staging Prognostication in Nasopharyngeal Carcinoma

2006 • 352 citations

Prediction of Breast Tumor Progression by Integrity of Free Circulating DNA in Serum

2006 • 335 citations

Molecular prognostication of nasopharyngeal carcinoma by quantitative analysis of circulating Epstein-Barr virus DNA.

2000 • 333 citations

Flow cytometric analysis of circulating microparticles in plasma

2010 • 324 citations

Increased Integrity of Free Circulating DNA in Sera of Patients with Colorectal or Periampullary Cancer: Direct Quantitative PCR for ALU Repeats

2006 • 304 citations

Quantification of circulating Epstein-Barr virus (EBV) DNA in the diagnosis and monitoring of natural killer cell and EBV-positive lymphomas in immunocompetent patients

2004 • 292 citations

Hypomethylation of a LINE-1 Promoter Activates an Alternate Transcript of the MET Oncogene in Bladders with Cancer

2010 • 278 citations

Phase I/II study of GSK2118436, a selective inhibitor of oncogenic mutant BRAF kinase, in patients with metastatic melanoma and other solid tumors.

2010 • 278 citations

Detection of free‐circulating tumor‐associated DNA in plasma of colorectal cancer patients and its association with prognosis

2002 • 273 citations

Active Alu retrotransposons in the human genome

2008 • 260 citations

Cell-Free Nucleic Acids Circulating in the Plasma of Colorectal Cancer Patients Induce the Oncogenic Transformation of Susceptible Cultured Cells

2010 • 248 citations

Molecular detection of genetic alterations in the serum of colorectal cancer patients.

1998 • 240 citations

Circulating Tumor-Specific DNA: A Marker for Monitoring Efficacy of Adjuvant Therapy in Cancer Patients

2005 • 239 citations

Cell-free Tumor DNA in Blood Plasma As a Marker for Circulating Tumor Cells in Prostate Cancer

2009 • 236 citations

Methylation of Endogenous Human Retroelements in Health and Disease

2006 • 219 citations

K-ras Mutations in DNA Extracted From the Plasma of Patients With Pancreatic Carcinoma: Diagnostic Utility and Prognostic Significance

1999 • 216 citations

Preoperative Serum DNA GSTP1 CpG Island Hypermethylation and the Risk of Early Prostate-Specific Antigen Recurrence Following Radical Prostatectomy

2005 • 206 citations

MicroRNA-500 as a potential diagnostic marker for hepatocellular carcinoma

2009 • 206 citations

PCR-Based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics, Prognostics, and Response to Treatment

2009 • 205 citations

Levels of plasma circulating cell free nuclear and mitochondrial DNA as potential biomarkers for breast tumors

2009 • 205 citations

Quantitative GSTP1 hypermethylation in bodily fluids of patients with prostate cancer

2002 • 203 citations

Quantitative Analysis of Circulating Mitochondrial DNA in Plasma

2003 • 194 citations

Nucleosomes in serum of patients with benign and malignant diseases

2001 • 187 citations

Performance of Epigenetic Markers SEPT9 and ALX4 in Plasma for Detection of Colorectal Precancerous Lesions

2010 • 185 citations

Frequent p15 promoter methylation in tumor and peripheral blood from hepatocellular carcinoma patients.

2000 • 181 citations

The role of macrophages in the in vitro generation of extracellular DNA from apoptotic and necrotic cells

2005 • 179 citations

Detection and Monitoring of Cell‐Free DNA in Blood of Patients with Colorectal Cancer

2008 • 178 citations

249ser TP53 mutation in plasma DNA, hepatitis B viral infection, and risk of hepatocellular carcinoma

2005 • 173 citations

Detection of mutant K-ras DNA in plasma or serum of patients with colorectal cancer

1997 • 172 citations

Molecular Detection of APC, K‐ras, and p53 Mutations in the Serum of Colorectal Cancer Patients as Circulating Biomarkers

2004 • 171 citations

Predictive Utility of Circulating Methylated DNA in Serum of Melanoma Patients Receiving Biochemotherapy

2005 • 169 citations

Quantitative Analysis of Circulating Methylated DNA as a Biomarker for Hepatocellular Carcinoma

2008 • 162 citations

Utility of Circulating B-RAF DNA Mutation in Serum for Monitoring Melanoma Patients Receiving Biochemotherapy

2007 • 159 citations

National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for Use of Tumor Markers in Clinical Practice: Quality Requirements1

2008 • 158 citations

Plasma cell‐free DNA in ovarian cancer

2010 • 157 citations

MicroRNAs as Novel Biomarkers for Breast Cancer

2009 • 154 citations

Cell-free and cell-bound circulating DNA in breast tumours: DNA quantification and analysis of tumour-related gene methylation

2006 • 150 citations

p16(INK4A) Hypermethylation detected by fluorescent methylation-specific PCR in plasmas from non-small cell lung cancer.

2002 • 145 citations

Circulating Nucleic Acids in Plasma and Serum

2006 • 143 citations

Clinical Relevance of Circulating Nucleosomes in Cancer

2008 • 139 citations

DNA‐Based Detection of Prostate Cancer in Blood, Urine, and Ejaculates

2001 • 136 citations

Circulating DNA as a Possible Factor in Oncogenesis

1965 • 134 citations

DNA Methylation in Serum and Tumors of Cervical Cancer Patients

2004 • 132 citations

Levels of Circulating Cell-Free Nuclear and Mitochondrial DNA in Benign and Malignant Ovarian Tumors

2008 • 130 citations

Persistent hepatitis B viral replication affects recurrence of hepatocellular carcinoma after curative resection

2007 • 129 citations

EGFR mutation status in tumour-derived DNA from pleural effusion fluid is a practical basis for predicting the response to gefitinib

2006 • 126 citations

Differential methylation of cell‐free circulating DNA among patients with pancreatic cancer versus chronic pancreatitis

2010 • 126 citations

Serum LINE-1 hypomethylation as a potential prognostic marker for hepatocellular carcinoma

2007 • 125 citations

Tumor-specific p53 sequences in blood and peritoneal fluid of women with epithelial ovarian cancer

2005 • 123 citations

Tumor DNA in plasma at diagnosis of breast cancer patients is a valuable predictor of disease-free survival.

2002 • 123 citations

Chemical regulation of epigenetic modifications: Opportunities for new cancer therapy

2008 • 122 citations

CpG Island hypermethylation in cell‐free serum DNA identifies patients with localized prostate cancer

2007 • 121 citations

14-3-3σ Methylation in Pretreatment Serum Circulating DNA of Cisplatin-Plus-Gemcitabine-Treated Advanced Non–Small-Cell Lung Cancer Patients Predicts Survival: The Spanish Lung Cancer Group

2005 • 119 citations

Multimarker Circulating DNA Assay for Assessing Blood of Prostate Cancer Patients

2009 • 117 citations

Circulating Nucleic Acids in Plasma and Serum

2008 • 117 citations

Biomarkers of apoptosis

2008 • 116 citations

Circulating Mitochondrial Nucleic Acids Have Prognostic Value for Survival in Patients with Advanced Prostate Cancer

2007 • 114 citations

Microsatellite analysis of free tumor DNA in urine, serum, and plasma of patients: a minimally invasive method for the detection of bladder cancer.

2002 • 113 citations

Human papillomavirus DNA in plasma of patients with cervical cancer

2001 • 113 citations

Plasma DNA microsatellites as tumor-specific markers and indicators of tumor progression in melanoma patients.

1999 • 112 citations

Quantification of Free Circulating Tumor DNA as a Diagnostic Marker for Breast Cancer

2008 • 110 citations

Circulating tumour‐associated plasma DNA represents an independent and informative predictor of prostate cancer

2006 • 109 citations

Diagnostic and prognostic implications of circulating cell-free Epstein-Barr virus DNA in natural killer/T-cell lymphoma.

2002 • 108 citations

Prospective Study of Quantitation of Plasma DNA Levels in the Diagnosis of Malignant versus Benign Prostate Disease

2005 • 105 citations

Suitable reference genes for relative quantification of miRNA expression in prostate cancer

2010 • 103 citations

Detection of p16 hypermethylation in circulating plasma DNA of non-small cell lung cancer patients

2002 • 102 citations

Detection of BRAF mutations in the tumour and serum of patients enrolled in the AZD6244 (ARRY-142886) advanced melanoma phase II study

2009 • 102 citations

Cell-Free Circulating DNA: Diagnostic Value in Patients With Testicular Germ Cell Cancer

2008 • 101 citations

Association of Circulating Tumor Cells with Serum Tumor-Related Methylated DNA in Peripheral Blood of Melanoma Patients

2006 • 100 citations

Potential Clinical Significance of a Plasma-Based KRAS Mutation Analysis in Patients with Advanced Non–Small Cell Lung Cancer

2010 • 99 citations

Circulating mitochondrial DNA in the serum of patients with testicular germ cell cancer as a novel noninvasive diagnostic biomarker

2009 • 98 citations

Effect of DNA size and strandedness on the in vivo clearance and organ localization of DNA.

1984 • 98 citations

The presence of circulating total DNA and methylated genes is associated with circulating tumour cells in blood from breast cancer patients

2009 • 95 citations

Noncancerous PTGS2 DNA fragments of apoptotic origin in sera of prostate cancer patients qualify as diagnostic and prognostic indicators

2007 • 94 citations

Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer

2010 • 93 citations

Role of Cell‐Free Plasma DNA as a Diagnostic Marker for Prostate Cancer

2004 • 93 citations

Origin and prognostic value of circulating KRAS mutations in lung cancer patients

2007 • 93 citations

Differential Methylation Profile of Ovarian Cancer in Tissues and Plasma

2008 • 92 citations

Quantification of LINE1 in Circulating DNA as a Molecular Biomarker of Breast Cancer

2008 • 92 citations

Amount of DNA in plasma and cancer risk: A prospective study

2004 • 92 citations

Detection of plasma tumor DNA in head and neck squamous cell carcinoma by microsatellite typing and p53 mutation analysis.

2000 • 91 citations

Apoptosis-related deregulation of proteolytic activities and high serum levels of circulating nucleosomes and DNA in blood correlate with breast cancer progression

2011 • 90 citations

Detection of aberrant promoter methylation of GSTP1 in the tumor and serum of Chinese human primary hepatocellular carcinoma patients

2006 • 90 citations

Mitochondrial DNA in serum of patients with prostate cancer: a predictor of biochemical recurrence after prostatectomy

2008 • 89 citations

Ser‐249TP53 mutation in tumour and plasma DNA of hepatocellular carcinoma patients from a high incidence area in the Gambia, West Africa

2004 • 89 citations

Plasma Cell‐free Epstein‐Barr Virus DNA Quantitation in Patients with Nasopharyngeal Carcinoma: Correlation with Clinical Staging

2000 • 87 citations

Clinical significance of promoter hypermethylation of DNA repair genes in tumor and serum DNA in invasive ductal breast carcinoma patients

2010 • 85 citations

Cell‐Surface‐Bound Nucleic Acids: Free and Cell‐Surface‐Bound Nucleic Acids in Blood of Healthy Donors and Breast Cancer Patients

2004 • 84 citations

Prognostic significance of circulating microsatellite markers in the plasma of melanoma patients.

2001 • 83 citations

Clinical usefulness of serum telomerase reverse transcriptase (hTERT) mRNA and epidermal growth factor receptor (EGFR) mRNA as a novel tumor marker for lung cancer

2006 • 83 citations

The prognostic value of circulating plasma DNA level and its allelic imbalance on chromosome 8p in patients with hepatocellular carcinoma

2006 • 78 citations

Methylation profile of circulating plasma DNA in patients with pancreatic cancer

2008 • 78 citations

Role of active cell death (apoptosis) in multi-stage carcinogenesis

1995 • 78 citations

Impact of platinum‐based chemotherapy on circulating nucleic acid levels, protease activities in blood and disseminated tumor cells in bone marrow of ovarian cancer patients

2010 • 77 citations

Promoter hypermethylation in circulating blood cells identifies prostate cancer progression

2007 • 77 citations

Assessing the Use of p16INK4a Promoter Gene Methylation in Serum for Detection of Bladder Cancer

2002 • 75 citations

Persistent Aberrations in Circulating DNA Integrity after Radiotherapy Are Associated with Poor Prognosis in Nasopharyngeal Carcinoma Patients

2008 • 73 citations

Free circulating mRNA in plasma from breast cancer patients and clinical outcome

2008 • 73 citations

Circulating nucleic acids in plasma and serum (CNAPS) and its relation to stem cells and cancer metastasis: state of the issue.

2004 • 72 citations

Methylation‐Specific Sequencing of GSTP1 Gene Promoter in Circulating/Extracellular DNA from Blood and Urine of Healthy Donors and Prostate Cancer Patients

2008 • 72 citations

Prediction of epidermal growth factor receptor mutations in the plasma/pleural effusion to efficacy of gefitinib treatment in advanced non-small cell lung cancer

2010 • 70 citations

Apoptotic DNA fragments in serum of patients with muscle invasive bladder cancer: A prognostic entity

2008 • 67 citations

CpG hypomethylation of MDR1 gene in tumor and serum of invasive ductal breast carcinoma patients

2009 • 66 citations

Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease

2010 • 64 citations

Circulating DNA Microsatellites: Molecular Determinants of Response to Biochemotherapy in Patients With Metastatic Melanoma

2004 • 63 citations

Hypermethylation of p16INK4a in Chinese lung cancer patients: biological and clinical implications

2003 • 61 citations

Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patients

2010 • 60 citations

Prevalence and distribution of human papillomavirus types in cervical cancer, squamous intraepithelial lesions, and with no intraepithelial lesions in women from Southern Mexico

2010 • 59 citations

Detection of tumor‐specific DNA in blood and bone marrow plasma from patients with prostate cancer

2007 • 58 citations

Comparative evaluation of cell-free tumor DNA in blood and disseminated tumor cells in bone marrow of patients with primary breast cancer

2009 • 55 citations

Detection of Microsatellite Alterations in the DNA Isolated from Tumor Cells and from Plasma DNA of Patients with Lung Cancer

2000 • 55 citations

Methods of Cancer Diagnosis, Therapy and Prognosis

2008 • 54 citations

Prognostic Value of Circulating Mutant DNA in Unresectable Metastatic Colorectal Cancer

2010 • 53 citations

Tumor p16M is a possible marker of advanced stage in non–small cell lung cancer

2002 • 53 citations

Allelic Imbalance on 12q22-23 in Serum Circulating DNA of Melanoma Patients Predicts Disease Outcome

2004 • 51 citations

Clinical Biomarkers in Oncology

2009 • 46 citations

Frequent copresence of methylated DNA and fragmented nucleosomal DNA in plasma of lymphoma patients

2003 • 42 citations

Evaluation of circulating thyroid‐specific transcripts as markers of thyroid cancer relapse

2004 • 41 citations

Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression

2010 • 41 citations

ChemInform Abstract: Chemical Regulations of Epigenetic Modifications: Opportunities for New Cancer Therapy

2008 • 38 citations

Analysis of Methylated Genes in Peritoneal Fluids of Ovarian Cancer Patients: A New Prognostic Tool

2004 • 38 citations

Microsatellite analysis of serum DNA in patients with head and neck cancer

2004 • 38 citations

Keeping Tabs on Tumor DNA

2010 • 37 citations

Prognostic value of circulating KRAS2 gene mutations in colorectal cancer with distant metastases

2006 • 35 citations

Circulating Nucleic Acids in Plasma and Serum

2010 • 34 citations

TP53 mutations in circulating free DNA from Egyptian patients with non-Hodgkin’s lymphoma

2008 • 33 citations

Prognostic Value of Circulating KRAS2 Gene Mutations in Colorectal Cancer with Distant Metastases

2006 • 33 citations

Comparative Analysis of Mesenteric and Peripheral Blood Circulating Tumor DNA in Colorectal Cancer Patients

2006 • 32 citations

Reduced Plasma RNA Integrity in Nasopharyngeal Carcinoma Patients

2006 • 30 citations

Detection of tumor-specific genetic alterations in bone marrow from early-stage breast cancer patients.

2003 • 30 citations

Feasibility and relevance of global expression profiling of gene transcripts in serum from breast cancer patients using whole genome microarrays and quantitative RT-PCR.

2008 • 29 citations

Circulating cell-free and Epstein–Barr virus DNA in pediatric B-non-Hodgkin lymphomas

2010 • 29 citations

Effect of Adjuvant Chemotherapy on Integrity of Free Serum DNA in Patients with Breast Cancer

2008 • 27 citations

Measurement of Cyclin E Genomic Copy Number and Strand Length in Cell-Free DNA Distinguish Malignant versus Benign Effusions

2007 • 25 citations

Extracellular DNA in Breast Cancer: Cell‐Surface‐Bound, Tumor‐Derived Extracellular DNA in Blood of Patients with Breast Cancer and Nonmalignant Tumors

2004 • 24 citations

Quantitative Analysis of Cell-Free Epstein-Barr Virus DNA in Plasma of Patients with Nonnasopharyngeal Head and Neck Carcinomas

2004 • 23 citations

Apoptosis and nucleosomes

2002 • 20 citations

Detection of Stage I nasopharyngeal carcinoma by serologic screening and clinical examination

2011 • 18 citations

Methylation status of the fragile histidine triad and E-cadherin genes in plasma of cervical cancer patients

2006 • 15 citations

Quantitative analysis of plasma HBV DNA for early evaluation of the response to transcatheter arterial embolization for HBV-related hepatocellular carcinoma

2005 • 10 citations

Circulating thyroglobulin mRNA does not predict early and midterm recurrences in patients undergoing thyroidectomy for cancer

2008 • 10 citations