The Mitochondrial Targeting function of Randomly Generated Peptide Sequences Correlates with Predicted Helical Amphiphilicity

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Statistics for Experimenters

1979 • 6,604 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

Transformation of yeast.

1978 • 2,267 citations

Analysis of membrane and surface protein sequences with the hydrophobic moment plot

1984 • 2,234 citations

Biogenesis of Mitochondria

1988 • 1,396 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

Mitochondrial protein import

1989 • 820 citations

Compilation of published signal sequences

1984 • 784 citations

The role of the α-helix dipole in protein function and structure

1985 • 517 citations

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

1986 • 493 citations

Protein Translocation Across Membranes

1988 • 482 citations

Transport of proteins into mitochondria: Translocational intermediates spanning contact sites between outer and inner membranes

1985 • 470 citations

Many Random Sequences Functionally Replace the Secretion Signal Sequence of Yeast Invertase

1987 • 375 citations

Mitochondrial presequences.

1988 • 357 citations

Saturation Mutagenesis of the Yeast his3 Regulatory Site: Requirements for Transcriptional Induction and for Binding by GCN4 Activator Protein

1986 • 352 citations

Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria.

1982 • 351 citations

How proteins find mitochondria and intramitochondrial compartments

1986 • 302 citations

Requirement of a Membrane Potential for the Posttranslational Transfer of Proteins into Mitochondsria

1982 • 292 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

Amphiphilicity is essential for mitochondrial presequence function.

1988 • 274 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

[58] Direct sequencing of denatured plasmid DNA

1987 • 246 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

Artificial mitochondrial presequences.

1986 • 203 citations

The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting

1986 • 194 citations

The cleavable pre-sequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria

1986 • 164 citations

Cloning of random-sequence oligodeoxynucleotides

1986 • 162 citations

Sequences from a prokaryotic genome or the mouse dihydrofolate reductase gene can restore the import of a truncated precursor protein into yeast mitochondria.

1987 • 160 citations

Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli

1975 • 158 citations

Mitochondrial protein import

1989 • 131 citations

Sequence Analysis in Molecular Biology: Treasure Trove or Trivial Pursuit

1987 • 128 citations

The precursor of beta-lactamase: purification, properties and folding kinetics.

1989 • 110 citations

A cytosolic protein contains a cryptic mitochondrial targeting signal

1987 • 101 citations

Structural analysis and amphiphilic properties of a chemically synthesized mitochondrial signal peptide.

1986 • 83 citations

Sequence analysis in molecular biology; Treasure trove or trivial pursuit

1988 • 82 citations

Sequence and structural requirements of a mitochondrial protein import signal defined by saturation cassette mutagenesis.

1989 • 69 citations

The yeast F1-ATPase beta subunit precursor contains functionally redundant mitochondrial protein import information.

1987 • 69 citations

Targeting efficiency of a mitochondrial pre-sequence is dependent on the passenger protein.

1986 • 60 citations

Independent mutations at the amino terminus of a protein act as surrogate signals for mitochondrial import.

1987 • 59 citations

Mitochondrial targeting sequences why ‘non‐amphiphilic’ peptides may still be amphiphilic

1988 • 57 citations

How finicky is mitochondrial protein import?

1988 • 47 citations

Identification of an outer mitochondrial membrane protein that interacts with a synthetic signal peptide.

1987 • 46 citations

Primary structure requirements for correct sorting of the yeast mitochondrial protein ADH III to the yeast mitochondrial matrix space.

1987 • 44 citations

Tight folding of a passenger protein can interfere with the targeting function of a mitochondrial presequence.

1989 • 32 citations

Different structures in the amino-terminal domain of the ornithine transcarbamylase leader peptide are involved in mitochondrial import and carboxyl-terminal cleavage.

1988 • 25 citations

Mutations restoring import of a yeast mitochondrial protein with a nonfunctional presequence.

1988 • 24 citations