A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

1969 • 1,847 citations

Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli

1981 • 1,732 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.

1967 • 1,317 citations

Mechanisms for the incorporation of proteins in membranes and organelles.

1982 • 875 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

5′-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency

1981 • 367 citations

Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

1982 • 281 citations

Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli

1980 • 268 citations

Assembly of the semliki forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro

1978 • 178 citations

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.

1981 • 177 citations

[53] Signal recognition particle: A ribonucleoprotein required for cotranslational translocation of proteins, isolation and properties

1983 • 155 citations

Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genes

1982 • 152 citations

Use of gene fusions to study outer membrane protein localization in Escherichia coli.

1977 • 148 citations

A bacterial secretory protein requires signal recognition particle for translocation across mammalian endoplasmic reticulum.

1982 • 118 citations

Xenopus oocytes can secrete bacterial β-lactamase

1984 • 79 citations

Efficient translation of prokaryotic mRNAs in a eukaryotic cell-free system requires addition of a cap structure

1979 • 75 citations

Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system.

1975 • 70 citations

Functional interaction of plant ribosomes with animal microsomal membranes

1977 • 69 citations

Cell-free coupled transcription-translation system for investigation of linear DNA segments.

1980 • 55 citations

Interaction of Escherichia coli RNA polymerase with promoters of several coliphage and plasmid DNAs.

1979 • 42 citations