The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

A complementation analysis of the restriction and modification of DNA in Escherichia coli

1969 • 3,866 citations

[1] Citrate synthase

1969 • 1,958 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

[10] Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast

1983 • 1,095 citations

[16] Plasmids of Escherichia coli as cloning vectors

1979 • 655 citations

IMPORT OF PROTEINS INTO MITOCHONDRIA

1991 • 583 citations

A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

1982 • 575 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

Membranes of mitochondria and chloroplasts

1970 • 477 citations

How mitochondria import proteins

1984 • 405 citations

Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria.

1982 • 351 citations

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

1985 • 327 citations

[13] Immunochemical identification of membrane proteins after sodium dodecyl sulfate—polyacrylamide gel electrophoresis

1983 • 309 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent.

1982 • 254 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor. Functional implications of the pre sequence for protein transport into mitochondria.

1982 • 249 citations

A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.

1985 • 239 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase

1967 • 235 citations

Import of proteins into mitochondria. Energy-dependent, two-step processing of the intermembrane space enzyme cytochrome b2 by isolated yeast mitochondria.

1982 • 220 citations

Nucleotide sequence of the yeast alcohol dehydrogenase II gene.

1983 • 208 citations

Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides.

1983 • 201 citations

The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix

1984 • 199 citations

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

1984 • 198 citations

Spectrophotometric quantitation of silver grains eluted from autoradiograms

1983 • 195 citations

Lactic dehydrogenase and cytochrome b2 of baker's yeast. Purification and crystallization

1959 • 187 citations

Sequencing of the nuclear gene for the yeast cytochrome c1 precursor reveals an unusually complex amino-terminal presequence.

1984 • 157 citations

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

1984 • 154 citations

Synthesis of polyoma proteins in vitro

1973 • 151 citations

Isolation and DNA Sequence of ADH3, a Nuclear Gene Encoding the Mitochondrial Isozyme of Alcohol Dehydrogenase in Saccharomyces cerevisiae

1985 • 146 citations

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

1985 • 140 citations

Import of proteins into mitochondria. Extramitochondrial pools and post-translational import of mitochondrial protein precursors in vivo.

1982 • 136 citations

Import of proteins into mitochondria. Translatable mRNAs for imported mitochondrial proteins are present in free as well as mitochondria-bound cytoplasmic polysomes.

1982 • 134 citations

Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.

1984 • 132 citations

The Primary Structure of Yeast Alcohol Dehydrogenase

1977 • 117 citations

Isolation and DNA sequence of ADH3, a nuclear gene encoding the mitochondrial isozyme of alcohol dehydrogenase in Saccharomyces cerevisiae.

1985 • 115 citations

Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.

1983 • 110 citations

Import of proteins into mitochondria. Import and maturation of the mitochondrial intermembrane space enzymes cytochrome b2 and cytochrome c peroxidase in intact yeast cells.

1982 • 102 citations

Biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. Discoordinate synthesis of the 11-kd subunit in response to increased gene copy number.

1983 • 92 citations

The biosynthesis of the ubiquinol‐cytochrome c reductase complex in yeast

1984 • 92 citations

Membrane‐Bound and Water‐Soluble Cytochrome c₁ From Neurospora Mitochondria

1981 • 75 citations

Intracellular sorting of alcohol dehydrogenase isoenzymes in yeast: a cytosolic location reflects absence of an amino-terminal targeting sequence for the mitochondrion.

1986 • 63 citations

Biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. Characterization of precursor forms of the 44-kDa, 40-kDa and 17-kDa subunits and identification of individual messenger RNAs for these and other imported subunits of the complex

1983 • 28 citations