The cleavable pre-sequence of an imported chloroplast protein directs attached polypeptides into yeast mitochondria

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

1982 • 575 citations

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

1986 • 493 citations

Organization and Expression of the Mitochondrial Genome

1999 • 377 citations

Transport of proteins into mitochondria and chloroplasts.

1979 • 355 citations

Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria.

1982 • 351 citations

Cytochrome c Oxidase from Bakers' Yeast

1973 • 331 citations

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

1985 • 327 citations

Transport of proteins into chloroplasts

1984 • 310 citations

How are proteins imported into mitochondria?

1983 • 307 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.

1985 • 239 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts

1985 • 233 citations

Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides.

1983 • 201 citations

The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix

1984 • 199 citations

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

1984 • 198 citations

Spectrophotometric quantitation of silver grains eluted from autoradiograms

1983 • 195 citations

The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting

1986 • 194 citations

On the Hydrophobic Nature of Signal Sequences

1981 • 193 citations

A neutral metallo endoprotease involved in the processing of an F1-ATPase subunit precursor in mitochondria.

1982 • 167 citations

Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli

1975 • 158 citations

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

1984 • 154 citations

NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii.

1979 • 142 citations

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

1985 • 140 citations

The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.

1985 • 120 citations

Functional determinants in transit sequences: import and partial maturation by vascular plant chloroplasts of the ribulose-1,5-bisphosphate carboxylase small subunit of Chlamydomonas.

1985 • 112 citations

Complete sequence of one of the mRNAs coding for the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris

1984 • 38 citations

Ochromonas mitochondria contain a specific chloroplast protein

1985 • 28 citations