Sequences from a prokaryotic genome or the mouse dihydrofolate reductase gene can restore the import of a truncated precursor protein into yeast mitochondria.

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

1977 • 2,408 citations

Analysis of membrane and surface protein sequences with the hydrophobic moment plot

1984 • 2,234 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

1986 • 493 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

Shotgun DNA sequencing using cloned DNase I-generated fragments

1981 • 425 citations

Has the endosymbiont hypothesis been proven?

1982 • 425 citations

How mitochondria import proteins

1984 • 405 citations

Many Random Sequences Functionally Replace the Secretion Signal Sequence of Yeast Invertase

1987 • 375 citations

Structure and Expression of a Complementary DNA for the Nuclear Coded Precursor of Human Mitochondrial Ornithine Transcarbamylase

1984 • 279 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA.

1982 • 205 citations

Artificial mitochondrial presequences.

1986 • 203 citations

Similar genes for a mitochondrial ATPase subunit in the nuclear and mitochondrial genomes of Neurospora crassa

1982 • 186 citations

Targeting of pre-ornithine transcarbamylase to mitochondria: Definition of critical regions and residues in the leader peptide

1986 • 172 citations

A neutral metallo endoprotease involved in the processing of an F1-ATPase subunit precursor in mitochondria.

1982 • 167 citations

Sequencing of the nuclear gene for the yeast cytochrome c1 precursor reveals an unusually complex amino-terminal presequence.

1984 • 157 citations

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

1984 • 154 citations

Isolation and DNA Sequence of ADH3, a Nuclear Gene Encoding the Mitochondrial Isozyme of Alcohol Dehydrogenase in Saccharomyces cerevisiae

1985 • 146 citations

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

1985 • 140 citations

Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.

1984 • 132 citations

Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron‐sulphur protein

1985 • 129 citations

The cytoplasmically-made subunit IV is necessary for assembly of cytochrome c oxidase in yeast.

1985 • 120 citations

A cytosolic protein contains a cryptic mitochondrial targeting signal

1987 • 101 citations

Biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. Discoordinate synthesis of the 11-kd subunit in response to increased gene copy number.

1983 • 92 citations

Isolation and sequence of the structural gene for cytochrome c oxidase subunit VI from Saccharomyces cerevisiae.

1984 • 92 citations

The Nine Amino-Terminal Residues of δ-Aminolevulinate Synthase Direct β-Galactosidase into the Mitochondrial Matrix

1986 • 67 citations

Independent mutations at the amino terminus of a protein act as surrogate signals for mitochondrial import.

1987 • 59 citations

Cloning and characterization of the yeast nuclear gene for subunit 5 of cytochrome oxidase.

1985 • 50 citations

The Primary Structure of the Imported Mitochondrial Protein, Ornithine Transcarbamylase From Rat Liver: mRNA Levels During Ontogeny

1985 • 28 citations