Independent mutations at the amino terminus of a protein act as surrogate signals for mitochondrial import.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Experiments With Gene Fusions

1984 • 2,487 citations

Sequence requirements for nuclear location of simian virus 40 large-T antigen

1984 • 1,278 citations

Experiments with gene fusions

1985 • 1,210 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

Methods in yeast genetics, laboratory manual

1987 • 733 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen

1984 • 566 citations

Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.

1984 • 513 citations

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

1986 • 493 citations

Transport of proteins into mitochondria: Translocational intermediates spanning contact sites between outer and inner membranes

1985 • 470 citations

In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent post-translational translocation of the prepro-α-factor

1986 • 332 citations

Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.

1986 • 315 citations

The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae

1986 • 308 citations

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.

1986 • 258 citations

A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.

1985 • 239 citations

Conformations of Signal Peptides Induced by Lipids Suggest Initial Steps in Protein Export

1986 • 225 citations

Secretion in yeast: Reconstitution of the translocation and glycosylation of α-factor and invertase in a homologous cell-free system

1986 • 216 citations

Targeting proteins into mitochondria

1986 • 171 citations

[6] Analysis of products of mitochondrial protein synthesis in yeast: Genetic and biochemical aspects

1979 • 160 citations

Isolation and characterization of MOD5, a gene required for isopentenylation of cytoplasmic and mitochondrial tRNAs of Saccharomyces cerevisiae.

1987 • 143 citations

The first twelve amino acids of a yeast mitochondrial outer membrane protein can direct a nuclear-coded cytochrome oxidase subunit to the mitochondrial inner membrane.

1985 • 140 citations

Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Primary sequence analysis of ATP2 encoding the F1-ATPase beta-subunit precursor.

1985 • 110 citations

Replacement of arginine 246 by histidine in the beta subunit of Escherichia coli H+-ATPase resulted in loss of multi-site ATPase activity.

1986 • 105 citations

Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria.

1984 • 102 citations

Fluorometric measurements on the interactton of melittin with lecithin

1973 • 92 citations

Sequences distal to the mitochondrial targeting sequences are necessary for the maturation of the F1-ATPase beta-subunit precursor in mitochondria.

1987 • 66 citations

Replacement of serine 373 by phenylalanine in the alpha subunit of Escherichia coli F1-ATPase results in loss of steady-state catalysis by the enzyme.

1984 • 55 citations

A phenylalanine for serine substitution in the beta subunit of Escherichia coli F1-ATPase affects dependence of its activity on divalent cations.

1984 • 47 citations

Isopentenylation of both cytoplasmic and mitochondrial tRNA is affected by a single nuclear mutation.

1982 • 41 citations

Isolation and Characterization of MODS, a Gene Required for Isopentenylation of Cytoplasmic and Mitochondrial tRNAs of Saccharomyces cerevisiae

1987 • 41 citations

Fusion of the Saccharomyces cerevisiae leu2 gene to an Escherichia coli beta-galactosidase gene.

1983 • 30 citations

A nuclear mutation altering the assembly of the energy-transducing membrane of yeast.

1979 • 29 citations