A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 • 47,127 citations

Solubilization of membranes by detergents

1975 • 2,909 citations

The Role of Polyamines in the Neutralization of Bacteriophage Deoxyribonucleic Acid

1960 • 2,598 citations

Determination of the helix and β form of proteins in aqueous solution by circular dichroism

1974 • 2,040 citations

Localization of mitochondria in living cells with rhodamine 123.

1980 • 1,529 citations

Phosphatidylcholine vesicles. Formation and physical characteristics

1969 • 1,522 citations

Use of Helical Wheels to Represent the Structures of Proteins and to Identify Segments with Helical Potential

1967 • 1,178 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction

1981 • 1,044 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

THREE-DIMENSIONAL STRUCTURE OF MEMBRANE AND SURFACE PROTEINS

1984 • 959 citations

Mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles

1974 • 949 citations

Lipids of mitochondria

1985 • 842 citations

Protein translocation across the endoplasmic reticulum

1984 • 825 citations

Multiple Mechanisms of Protein Insertion into and Across Membranes

1985 • 720 citations

Liposome-Cell Interaction: Transfer and Intracellular Release of a Trapped Fluorescent Marker

1977 • 702 citations

Membrane fusion proteins of enveloped animal viruses

1983 • 670 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Estimation of Membrane Potential and pH Difference across the Cristae Membrane of Rat Liver Mitochondria

1969 • 589 citations

Amphiphilic Secondary Structure: Design of Peptide Hormones

1984 • 541 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

Transport of proteins into mitochondria: Translocational intermediates spanning contact sites between outer and inner membranes

1985 • 470 citations

Peptides. Structure and function.

1973 • 455 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Import of proteins into mitochondria. Energy-dependent uptake of precursors by isolated mitochondria.

1982 • 351 citations

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

1985 • 327 citations

Secondary structures of proteins and peptides in amphiphilic environments. (A review).

1983 • 299 citations

Requirement of a Membrane Potential for the Posttranslational Transfer of Proteins into Mitochondsria

1982 • 292 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria.

1975 • 262 citations

Transport of proteins into mitochondria: a potassium diffusion potential is able to drive the import of ADP/ATP carrier.

1985 • 245 citations

A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.

1985 • 239 citations

Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides.

1983 • 201 citations

The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix

1984 • 199 citations

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

1984 • 198 citations

The presequences of two imported mitochondrial proteins contain information for intracellular and intramitochondrial sorting

1986 • 194 citations

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

1984 • 154 citations

Penetration of phospholipid monolayers by cardiotoxins

1981 • 153 citations

Yeast mitochondrial outer membrane specifically binds cytoplasmically-synthesized precursors of mitochondrial proteins

1983 • 149 citations

Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes.

1984 • 149 citations

Proteinaceous receptors for the import of mitochondrial precursor proteins.

1984 • 148 citations

A microchemical facility for the analysis and synthesis of genes and proteins

1984 • 134 citations

In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides

1985 • 130 citations

The Enzymes of Biological Membranes

1985 • 122 citations

Exploring the conformational roles of signal sequences: synthesis and conformational analysis of .lambda. receptor protein wild-type and mutant signal peptides

1984 • 117 citations

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

1985 • 107 citations

A synthetic signal peptide blocks import of precursor proteins destined for the mitochondrial inner membrane or matrix.

1985 • 89 citations

Effects of Synthetic Model Peptides Resembling the Extension Peptides of Mitochondrial Enzyme Precursors on Import of the Precursors into Mitochondria1

1985 • 75 citations

Insertion of apocytochrome c into lipid vesicles.

1984 • 71 citations

Is the mitochondrial precursor protein apocytochrome c able to pass a lipid barrier?

1984 • 67 citations

Intracellular sorting of alcohol dehydrogenase isoenzymes in yeast: a cytosolic location reflects absence of an amino-terminal targeting sequence for the mitochondrion.

1986 • 63 citations

Melittin‐induced fusion of acidic liposomes

1983 • 58 citations

Interaction of Staphylococcus aureus .delta.-lysin with phospholipid monolayers

1982 • 48 citations

Purification of a trypsin-insensitive fragment of spectrin from human erythrocyte membranes

1981 • 23 citations

Conversion of a Cary 60 spectropolarimeter into a fast circular dichroism instrument for use with standard rapid reaction techniques

1979 • 20 citations