Analysis of mammalian 20S proteasome biogenesis: the maturation of beta-subunits is an ordered two-step mechanism involving autocatalysis.

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Inhibition of Proteasome Activities and Subunit-Specific Amino-Terminal Threonine Modification by Lactacystin

1995 • 1,601 citations

Crystal Structure of the 20 S Proteasome from the Archaeon T. acidophilum at 3.4 Å Resolution

1995 • 1,513 citations

Serine Proteases: Structure and Mechanism of Catalysis

1977 • 1,238 citations

Ubiquitin, proteasomes, and the regulation of intracellular protein degradation

1995 • 862 citations

A 26 S protease subunit that binds ubiquitin conjugates.

1994 • 759 citations

Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels

1988 • 697 citations

A protein catalytic framework with an N-terminal nucleophile is capable of self-activation

1995 • 609 citations

Proteolysis, proteasomes and antigen presentation

1992 • 576 citations

The multicatalytic and 26 S proteases.

1993 • 476 citations

Penicillin acylase has a single-amino-acid catalytic centre

1995 • 448 citations

Impaired assembly and transport of HLA-A and -B antigens in a mutant TxB cell hybrid.

1986 • 437 citations

A proteasome-related gene between the two ABC transporter loci in the class II region of the human MHC

1991 • 427 citations

Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.

1991 • 421 citations

Proteasomes: multicatalytic proteinase complexes

1993 • 395 citations

Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids

1993 • 323 citations

[28] Cleavage at aspartic acid

1983 • 238 citations

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

1992 • 235 citations

Structure of the Allosteric Regulatory Enzyme of Purine Biosynthesis

1994 • 235 citations

Autocatalytic processing of the 20S proteasome

1996 • 226 citations

Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex.

1994 • 220 citations

Critical elements in proteasome assembly

1994 • 203 citations

Identification of MECL-1 (LMP-10) as the third IFN-gamma-inducible proteasome subunit.

1996 • 196 citations

Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma.

1996 • 195 citations

Proteolytic processing and physiological regulation

1989 • 195 citations

In Vivo Assembly of the Proteasomal Complexes, Implications for Antigen Processing

1995 • 193 citations

The first characterization of a eubacterial proteasome: the 20S complex of Rhodococcus

1995 • 186 citations

cDNA Cloning and Interferon γ Down-Regulation of Proteasomal Aubunits X and Y

1994 • 181 citations

Subunit stoichiometry and three-dimensional arrangement in proteasomes from Thermoplasma acidophilum.

1992 • 180 citations

Activation of Glycosylasparaginase

1996 • 180 citations

Existence of a molecular ruler in proteasomes suggested by analysis of degradation products

1994 • 179 citations

A third interferon‐γ‐induced subunit exchange in the 20S proteasome

1996 • 168 citations

Incorporation of major histocompatibility complex – encoded subunits LMP2 and LMP7 changes the quality of the 20S proteasome polypeptide processing products independent of interferon‐γ

1995 • 167 citations

The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.

1993 • 149 citations

20 S proteasomes are assembled via distinct precursor complexes processing of LMP2 and LMP7 proproteins takes place in 13–16 S preproteasome complexes

1994 • 146 citations

Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 å crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase

1996 • 143 citations

Kinetic Characterization of the Chymotryptic Activity of the 20S Proteasome

1996 • 137 citations

Genes encoded in the major histocompatibility complex affecting the generation of peptides for TAP transport

1995 • 129 citations

Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1991 • 123 citations

Alternative exon usage and processing of the major histocompatibility complex-encoded proteasome subunits.

1992 • 119 citations

Biogenesis, structure and function of the yeast 20S proteasome.

1995 • 115 citations

Evidence Indicating that the Human Proteasome is a Complex Dimer

1993 • 97 citations

Functional analyses of active site residues of human lysosomal aspartylglucosaminidase: implications for catalytic mechanism and autocatalytic activation.

1996 • 88 citations

Molecular cloning and sequence analysis of cDNAs for five major subunits of human proteasomes (multi-catalytic proteinase complexes)

1991 • 81 citations

PRE3, highly homologous to the human major histocompatibility complex‐linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl‐peptide hydrolyzing activity

1994 • 80 citations

Biochemical properties of the proteasome from Thermoplasma acidophilum

1992 • 78 citations

A Proteasome from the Methanogenic Archaeon Methanosarcina thermophila

1995 • 70 citations

The major‐histocompatibility‐complex‐encoded β‐type proteasome subunits LMP2 and LMP7

1993 • 61 citations

Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine.

1994 • 56 citations

Synthetic Inhibitors of the Multicatalytic Proteinase Complex (Proteasome)

1993 • 55 citations

Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits

1994 • 53 citations

The proteasome from Thermoplasma acidophilum is neither a cysteine nor a serine protease

1995 • 51 citations

The human proteasome subunit HsN3 is located inthe inner rings of the complex dimer

1995 • 50 citations

The Structural and Functional Organization of Intramolecular Chaperones: The N-Terminal Propeptides Which Mediate Protein Folding1

1994 • 47 citations

Catalytic Components of the Bovine Pituitary Multicatalytic Proteinase Complex (Proteasome)

1993 • 44 citations

Sequence analyses and inter-species comparisons of three novel human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine potential proteolytic active-site residues

1994 • 34 citations

Nucleotide sequence ofPUP1encoding a putative proteasome subunit inSaccharomyces cerevisiae

1991 • 33 citations

PRS3 encoding an essential subunit of yeast proteasomes homologous to mammalian proteasome subunit C5

1992 • 29 citations