Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p.

Antibodies, a laboratory manual

1989 • 12,929 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion

1993 • 997 citations

Identification and characterization of a nuclear pore complex protein

1986 • 610 citations

How proteins enter the nucleus

1991 • 555 citations

Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.

1987 • 533 citations

[35] Epitope tagging and protein surveillance

1991 • 526 citations

Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.

1990 • 510 citations

Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.

1987 • 510 citations

Architecture and design of the nuclear pore complex

1992 • 452 citations

Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.

1991 • 442 citations

Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.

1987 • 428 citations

Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy

1993 • 408 citations

Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import

1991 • 391 citations

Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.

1992 • 370 citations

Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.

1987 • 330 citations

A large particle associated with the perimeter of the nuclear pore complex.

1982 • 314 citations

Genetic analysis of the mitotic transmission of minichromosomes

1985 • 310 citations

A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm

1993 • 308 citations

O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins.

1987 • 268 citations

A new family of yeast nuclear pore complex proteins.

1992 • 256 citations

Toward a more complete 3-D structure of the nuclear pore complex

1991 • 254 citations

Structure and Function of the Nuclear Pore Complex

1992 • 237 citations

Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells

1987 • 233 citations

A complex of nuclear pore proteins required for pore function.

1991 • 231 citations

A yeast mutant which accumulates precursor tRNAs

1978 • 231 citations

Reconstitution of biochemically altered nuclear pores: Transport can be eliminated and restored

1990 • 228 citations

The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.

1990 • 228 citations

High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.

1992 • 225 citations

An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region

1993 • 220 citations

The NUP1 gene encodes an essential component of the yeast nuclear pore complex

1990 • 219 citations

Protein import through the nuclear pore complex is a multistep process.

1989 • 208 citations

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

1988 • 206 citations

Temperature-sensitive Yeast Mutant Defective in Ribonucleic Acid Production

1969 • 201 citations

Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.

1989 • 193 citations

Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae.

1988 • 191 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

NSP1: A yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain

1990 • 180 citations

Classical mutagenesis techniques

2002 • 176 citations

Cloning and Characterization of SRP1, a Suppressor of Temperature-Sensitive RNA Polymerase I Mutations, in Saccharomyces cerevisiae

1992 • 175 citations

A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.

1992 • 172 citations

The nuclear pore complex

1993 • 166 citations

Use of a Screen for Synthetic Lethal and Multicopy Suppressee Mutants To Identify Two New Genes Involved in Morphogenesis in Saccharomyces cerevisiae

1991 • 166 citations

Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin

1988 • 146 citations

[21] Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors

1991 • 146 citations

Primary sequence and heterologous expression of nuclear pore glycoprotein p62.

1990 • 137 citations

Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region.

1991 • 123 citations

NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.

1993 • 119 citations

Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins.

1989 • 112 citations

Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.

1991 • 104 citations

The Role of Cytoplasmic Membranes in Controlling the Transport of Nuclear Messenger RNA and Initiation of Protein Synthesis

1974 • 86 citations

In vitro translocation through the yeast nuclear envelope

1989 • 63 citations

An RNA polymerase II mutation in Drosophila melanogaster that mimics ultrabithorax

1981 • 62 citations

Genetic evidence that the Tat proteins of human immunodeficiency virus types 1 and 2 can multimerize in the eukaryotic cell nucleus

1993 • 57 citations

NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation.

1992 • 53 citations

Control of nucleocytoplasmic transport

1992 • 26 citations