Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility

Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.

1996 • 1,917 citations

Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis

1990 • 1,239 citations

SELF-SPLICING OF GROUP I INTRONS

1990 • 852 citations

Comparative and functional anatomy of group II catalytic introns — a review

1989 • 768 citations

INTRONS AS MOBILE GENETIC ELEMENTS

1993 • 618 citations

STRUCTURE AND ACTIVITIES OF GROUP II INTRONS

1995 • 569 citations

Split genes and RNA splicing

1994 • 562 citations

Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron

1980 • 477 citations

Group I introns as mobile genetic elements: Facts and mechanistic speculations — a review

1989 • 473 citations

Homing endonucleases: keeping the house in order

1997 • 466 citations

Conserved sequences and structures of group I introns: building an active site for RNA catalysis — a review

1988 • 451 citations

The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.

1993 • 437 citations

Zinc Finger Phage: Affinity Selection of Fingers with New DNA-Binding Specificities

1994 • 417 citations

An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene

1985 • 378 citations

Recurrent invasion and extinction of a selfish gene

1999 • 378 citations

Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the ω and rib-1 loci

1980 • 373 citations

A General Strategy for Selecting High-Affinity Zinc Finger Proteins for Diverse DNA Target Sites

1997 • 327 citations

Recognition and Cleavage of DNA by Type‐II Restriction Endonucleases

1997 • 326 citations

A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae

1983 • 302 citations

In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence

1994 • 290 citations

Explosive invasion of plant mitochondria by a group I intron

1998 • 280 citations

Group II intron mobility occurs by target DNA-primed reverse transcription

1995 • 278 citations

Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae

1992 • 275 citations

Group I and group II introns.

1993 • 268 citations

Universal code equivalent of a yeast mitochondrial intron reading frame is expressed into E. coli as a specific double strand endonuclease

1986 • 266 citations

Structure of the multimodular endonuclease FokI bound to DNA

1997 • 259 citations

Crystal Structure of PI-SceI, a Homing Endonuclease with Protein Splicing Activity

1997 • 254 citations

Mechanisms of Intron Mobility

1995 • 248 citations

DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI

1998 • 237 citations

Use of a zinc-finger consensus sequence framework and specificity rules to design specific DNA binding proteins.

1993 • 227 citations

Mg2+ Binding to the Active Site of EcoRV Endonuclease: A Crystallographic Study of Complexes with Substrate and Product DNA at 2-.ANG. Resolution

1995 • 222 citations

A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron

1997 • 219 citations

Zinc finger peptides for the regulation of gene expression

1999 • 217 citations

Retrohoming of a Bacterial Group II Intron

1998 • 213 citations

A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility

1995 • 212 citations

Group II Introns Designed to Insert into Therapeutically Relevant DNA Target Sites in Human Cells

2000 • 204 citations

Compilation and analysis of intein sequences

1997 • 204 citations

Statistical modeling and analysis of the LAGLIDADG family of site- specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family

1997 • 190 citations

The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein

1999 • 186 citations

Mitochondrial genetics. I. Methodology and phenomenology.

1970 • 172 citations

Colicins: structures, modes of action, transfer through membranes, and evolution

1994 • 166 citations

Structural and mechanistic basis of immunity toward endonuclease colicins.

1999 • 165 citations

Efficient integration of an intron RNA into double-stranded DNA by reverse splicing

1996 • 163 citations

An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA

1978 • 162 citations

Homing endonucleases: structure, function and evolution

1999 • 161 citations

Improved DNA binding specificity from polyzinc finger peptides by using strings of two-finger units

2001 • 159 citations

Transposition of an intron in yeast mitochondria requires a protein encoded by that intron

1985 • 159 citations

A mobile group I intron in the nuclear rDNA of physarum polycephalum

1989 • 158 citations

The role of metals in catalysis by the restriction endonuclease BamHI

1998 • 156 citations

Modular organization of inteins and C‐terminal autocatalytic domains

1998 • 149 citations

DNA Recognition and Cleavage by the LAGLIDADG Homing Endonuclease I-Cre I

1998 • 148 citations

Retrotransposition of a bacterial group II intron

2000 • 146 citations

Amino acid sequence motif of group I intron endonucleases is conserved in open reading frames of group II introns

1994 • 146 citations

Invasion of a multitude of genetic niches by mobile endonuclease genes

2000 • 141 citations

Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences

2000 • 137 citations

X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture

1999 • 135 citations

The structure of I-Crel, a Group I intron-encoded homing endonuclease

1997 • 130 citations

Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci

1996 • 125 citations

I- Ppo I and I- Cre I homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment 1 1Edited by G. Smith

1998 • 121 citations

A novel endonuclease mechanism directly visualized for I-PpoI.

1999 • 118 citations

Mobile introns: definition of terms and recommended nomenclature

1989 • 117 citations

Crystal Structure of an Archaeal Intein-encoded Homing Endonuclease PI-PfuI

2000 • 114 citations

Mobility of Yeast Mitochondrial Group II Introns: Engineering a New Site Specificity and Retrohoming via Full Reverse Splicing

1997 • 114 citations

Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution.

2000 • 114 citations

mRNA splicing and autocatalytic introns: Distant cousins or the products of chemical determinism?

1993 • 112 citations

Configuration of the catalytic GIY-YIG domain of intron endonuclease I-TevI: coincidence of computational and molecular findings

1999 • 112 citations

Archaeal introns: splicing, intercellular mobility and evolution

1997 • 112 citations

Self‐splicing group I and group II introns encode homologous (putative) DNA endonucleases of a new family

1994 • 111 citations

Structural parsimony in endonuclease active sites: should the number of homing endonuclease families be redefined?

1999 • 110 citations

Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene

1982 • 108 citations

Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease

1995 • 107 citations

Beyond Homing: Competition between Intron Endonucleases Confers a Selective Advantage on Flanking Genetic Markers

1996 • 106 citations

Intron mobility in phage T4 is dependent upon a distinctive class of endonucleases and independent of DNA sequences encoding the intron core: mechanistic and evolutionary implications

1990 • 105 citations

The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.

2001 • 101 citations

Characterization of I-Ppo, an Intron-Encoded Endonuclease That Mediates Homing of a Group I Intron in the Ribosomal DNA of Physarum polycephalum

1990 • 99 citations

Substrate Recognition and Induced DNA Distortion by the PI-SceI Endonuclease, an Enzyme Generated by Protein Splicing

1996 • 98 citations

Crystal structure of the thermostable archaeal intron-encoded endonuclease I- Dmo I 1 1Edited by I. A. Wilson

1999 • 96 citations

Mutations affecting RNA splicing and the interaction of gene expression of the yeast mitochondrial loci cob and oxi-3

1980 • 91 citations

Two-domain structure of the td intron-encoded endonuclease I-TevI correlates with the two-domain configuration of the homing site

1997 • 90 citations

Identification of a family of bacteriophage T4 genes encoding proteins similar to those present in group I introns of fungi and phage.

1992 • 87 citations

A self-splicing group I intron in the DNA polymerase gene of bacillus subtilis bacteriophage SPO1

1990 • 87 citations

The active site of Serratia endonuclease contains a conserved magnesium-water cluster

1999 • 84 citations

Evolutionary transfer of ORF-containing group I introns between different subcellular compartments (chloroplast and mitochondrion).

1995 • 84 citations

Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos.

1998 • 81 citations

A site-specific endonuclease encoded by a typical archaeal intron.

1993 • 80 citations

The td intron endonuclease I-TevI makes extensive sequence-tolerant contacts across the minor groove of its DNA target.

1993 • 75 citations

Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases

2001 • 74 citations

The phage T4 nrdB intron: a deletion mutant of a version found in the wild.

1991 • 73 citations

Splicing of a group II intron in a functional transfer gene of Lactococcus lactis

1996 • 72 citations

Site-specific cleavage of DNA–RNA hybrids by zinc finger/FokI cleavage domain fusions

1997 • 71 citations

Evolution of mobile group I introns: recognition of intron sequences by an intron-encoded endonuclease.

1994 • 68 citations

Design of polyzinc finger peptides with structured linkers

2001 • 68 citations

The I-CeuI endonuclease recognizes a sequence of 19 base pairs and preferentially cleaves the coding strand of theChlamydomonas moewusiichloroplast large subunit rRNA gene

1992 • 68 citations

Cleavage and recognition pattern of a double-strand-specific endonuclease (I-CreI) encoded by the chloroplast 23S rRNA intron of Chlamydomonas reinhardtii

1992 • 68 citations

Intron-encoded endonuclease I-TevI binds as a monomer to effect sequential cleavage via conformational changes in the td homing site.

1995 • 66 citations

A similar active site for non-specific and specific endonucleases.

1999 • 64 citations

A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease

1997 • 64 citations

A family of nuclear homing endonucleases

1993 • 62 citations

In vivo mobility of a group I twintron in nuclear ribosomal DNA of the myxomycete Didymium iridis

1997 • 62 citations

The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate

1999 • 61 citations

I-TevI, the endonuclease encoded by the mobile td intron, recognizes binding and cleavage domains on its DNA target.

1991 • 60 citations

Selection of a Remote Cleavage Site by I-TevI, thetdIntron-encoded Endonuclease

1995 • 59 citations

Amino Acid Residues in Both the Protein Splicing and Endonuclease Domains of the PI-SceI Intein Mediate DNA Binding

1998 • 55 citations

Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI

1996 • 54 citations

Chimeric Restriction Enzyme: Gal4 Fusion to Fokl Cleavage Domain

1998 • 54 citations

Replacement of two non-adjacent amino acids in the S.cerevisiae bi2 intron-encoded RNA maturase is sufficient to gain a homing-endonuclease activity.

1996 • 52 citations

Non-canonical inteins

1998 • 50 citations

The DNA polymerase genes of several HMU-bacteriophages have similar group I introns with highly divergent open reading frames

1994 • 50 citations

Genetic Analysis of the Chlamydomonas reinhadtii I-CreI Mobile Intron Homing System in Escherichia coli

1997 • 48 citations

Analysis of the HO-cleaved MAT DNA intermediate generated during the mating type switch in the yeast Saccharomyces cerevisiae.

1989 • 48 citations

Conformational Changes and Cleavage by the Homing Endonuclease I-PpoI: A Critical Role for a Leucine Residue in the Active Site

2000 • 48 citations

Substrate Binding and Turnover by the Highly Specific I-PpoI Endonuclease

1996 • 48 citations

The beta-Ribbon DNA Recognition Motif

1994 • 45 citations

Interaction of the intron-encoded mobility endonuclease I-PpoI with its target site.

1993 • 45 citations

Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

1998 • 43 citations

Interspecific transfer of mitochondrial genes in fungi and creation of a homologous hybrid gene.

1994 • 42 citations

Incipient mitochondrial evolution in yeasts

1991 • 42 citations

Evolutionarily conserved and functionally important residues in the I- CeuI homing endonuclease

1997 • 41 citations

The maturase encoded by a group I intron from Aspergillus nidulans stabilizes RNA tertiary structure and promotes rapid splicing 1 1Edited by D. E. Draper

1999 • 39 citations

A new class of site-specific endodeoxyribonucleases. Endo.Sce I isolated from a eukaryote, Saccharomyces cerevisiae.

1983 • 39 citations

Incipient mitochondrial evolution in yeasts

1991 • 39 citations

The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding

1998 • 39 citations

Chemical Mechanism of DNA Cleavage by the Homing Endonuclease I-PpoI

1999 • 37 citations

Mapping metal ions at the catalytic centres of two intron-encoded endonucleases

1997 • 37 citations

Functional characterization of isoschizomeric His‐Cys box homing endonucleases from Naegleria

2000 • 36 citations

Probing the Structure of the PI-SceI-DNA Complex by Affinity Cleavage and Affinity Photocross-linking

2000 • 36 citations

Degenerate DNA recognition by I-PpoI endonuclease

1998 • 36 citations

Cleavage experiments with deoxythymidine 3′,5′‐bis‐(p‐nitrophenyl phosphate) suggest that the homing endonuclease I‐PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non‐specific Serratia nuclease¹

1999 • 35 citations

A mitochondrial group-I intron in fission yeast encodes a maturase and is mobile in crosses

1994 • 35 citations

Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility

2000 • 34 citations

Protein engineering of the restriction endonuclease EcoRV

1994 • 33 citations

PI-PfuI and PI-Pfull, intein-coded homing endonucleases from Pyrococcus furiosus. II. Characterization of the binding and cleavage abilities by site-directed mutagenesis

1999 • 32 citations

Intron-encoded Endonuclease I-TevII Binds Across the Minor Groove and Induces Two Distinct Conformational Changes in its DNA Substrate

1996 • 31 citations

Biochemical characterization of I-CmoeI reveals that this H-N-H homing endonuclease shares functional similarities with H-N-H colicins

2000 • 29 citations

The catalytic group-I introns of the psbA gene of Chlamydomonas reinhardtii : core structures, ORFs and evolutionary implications

1999 • 29 citations

I‐NjaI, a nuclear intron‐encoded homing endonuclease from Naegleria, generates a pentanucleotide 3′ cleavage‐overhang within a 19 base‐pair partially symmetric DNA recognition site

1999 • 29 citations

Identification of Lys-403 in the PI-SceI Homing Endonuclease as Part of a Symmetric Catalytic Center

1998 • 29 citations

Ethnomethodology and phenomenology

1968 • 27 citations

Major Groove Recognition by Three-stranded β-Sheets: Affinity Determinants and Conserved Structural Features

2000 • 25 citations

Identification of Asp218 and Asp326 as the Principal Mg²⁺ Binding Ligands of the Homing Endonuclease PI-SceI^,

2000 • 24 citations

Site-Specific Endo-Deoxyribonucleases in Eukaryotes: Endonucleases of YeastsSaccharomyces and Pichia1

1981 • 22 citations

EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodexynucleotides

1996 • 19 citations

A Model for the PI-SceI×DNA Complex Based on Multiple Base and Phosphate Backbone-specific Photocross-links

2000 • 15 citations

Purification and characterization of the DNA cleavage and recognition site of I-ScaI mitochondrial group I intron encoded endonuclease produced in Escherichia coli

2000 • 14 citations

Interaction of the Intron-Encoded Mobility Endonuclease I-PpoI with its Target Site

1993 • 13 citations

The apocytochrome-b gene in Chlorogonium elongatum (Chlamydomonadaceae): an intronic GIY-YIG ORF in green algal mitochondria

1997 • 11 citations

Intron-encoded open reading frame of the GIY-YIG subclass in a plastid gene

1995 • 7 citations

Mass migration of a group I intron: Promiscuity on a grand scale

1998 • 5 citations