Protein engineering of the restriction endonuclease EcoRV

Enzyme structure and mechanism

1977 • 2,935 citations

Enzyme structure and mechanism

1978 • 2,897 citations

Validation of the general purpose tripos 5.2 force field

1989 • 2,743 citations

The gapped duplex DNA approach to oligonucleotide-directed mutation construction

1984 • 511 citations

Structural studies of protein–nucleic acid interaction: the sources of sequence-specific binding

1990 • 508 citations

The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.

1993 • 437 citations

Refinement of Eco RI Endonuclease Crystal Structure: A Revised Protein Chain Tracing

1990 • 339 citations

Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease

1987 • 169 citations

EcoRV restriction endonuclease binds all DNA sequences with equal affinity

1991 • 139 citations

Structure and function of restriction endonucleases

1991 • 132 citations

Restriction endonucleases and modification methylases

1993 • 103 citations

Structural aspects of protein-DNA recognition

1991 • 96 citations

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis

1991 • 81 citations

Structure and function of restriction endonucleases

1992 • 73 citations

Magnesium(2+) confers DNA binding specificity to the EcoRV restriction endonuclease

1992 • 71 citations

On the catalytic mechanism of EcoRI and EcoRV A detailed proposal based on biochemical results, structural data and molecular modelling

1992 • 67 citations

Interaction of the EcoRV restriction endonuclease with the deoxyadenosine and thymidine bases in its recognition hexamer d(GATATC)

1990 • 67 citations

Purification and crystallization of the EcoRV restriction endonuclease.

1985 • 67 citations

Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.

1990 • 64 citations

Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases

1986 • 61 citations

Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides

1988 • 55 citations

Mutational Analysis of the Function of Gln115 in the EcoRI Restriction Endonuclease, a Critical Amino Acid for Recognition of the Inner Thymidine Residue in the Sequence -GAATTC- and for Coupling Specific DNA Binding to Catalysis

1993 • 41 citations

Structure–function correlation for the EcoRV restriction enzyme: from non‐specific binding to specific DNA cleavage

1993 • 33 citations

Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants

1989 • 31 citations

Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases

1986 • 30 citations

Effects of functional group changes in the EcoRV recognition site on the cleavage reaction catalyzed by the endonuclease

1989 • 22 citations