Probing the Structure of the PI-SceI-DNA Complex by Affinity Cleavage and Affinity Photocross-linking

[20] Processing of X-ray diffraction data collected in oscillation mode

1997 • 33,348 citations

Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical features

1983 • 14,802 citations

Improved methods for building protein models in electron density maps and the location of errors in these models

1991 • 12,831 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

X-PLOR Version 3.1: A System for X-ray Crystallography and NMR

1992 • 3,488 citations

The Definition of Generalized Helicoidal Parameters and of Axis Curvature for Irregular Nucleic Acids

1988 • 895 citations

Protein Splicing Converts the Yeast TFP1 Gene Product to the 69-kdDSubunit of the Vacuolar H ⁺ -Adenosine Triphosphatase

1990 • 485 citations

Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.

1990 • 474 citations

Homing endonucleases: keeping the house in order

1997 • 466 citations

Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae

1992 • 275 citations

Crystal Structure of a Hedgehog Autoprocessing Domain: Homology between Hedgehog and Self-Splicing Proteins

1997 • 274 citations

Crystal Structure of PI-SceI, a Homing Endonuclease with Protein Splicing Activity

1997 • 254 citations

DNA Recognition and Cleavage by the LAGLIDADG Homing Endonuclease I-Cre I

1998 • 148 citations

Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution

1997 • 137 citations

The structure of I-Crel, a Group I intron-encoded homing endonuclease

1997 • 130 citations

Incorporation of an EDTA-metal complex at a rationally selected site within a protein: application to EDTA-iron DNA affinity cleaving with catabolite gene activator protein (CAP) and Cro

1992 • 109 citations

Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease

1995 • 107 citations

Substrate Recognition and Induced DNA Distortion by the PI-SceI Endonuclease, an Enzyme Generated by Protein Splicing

1996 • 98 citations

Crystal structure of the thermostable archaeal intron-encoded endonuclease I- Dmo I 1 1Edited by I. A. Wilson

1999 • 96 citations

Structural Motif of the GCN4 DNA Binding Domain Characterized by Affinity Cleaving

1990 • 91 citations

Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors

1997 • 79 citations

Statistical Modeling, Phylogenetic Analysis and Structure Prediction of a Protein Splicing Domain Common to Inteins and Hedgehog Proteins

1997 • 74 citations

The two α subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact different halves of the DNA upstream element

1997 • 65 citations

Purification and characterization of VDE, a site-specific endonuclease from the yeast Saccharomyces cerevisiae.

1993 • 64 citations

Synthesis of the Protein Cutting Reagent Iron (S)-1-(p-Bromoacetamidobenzyl)ethylenediaminetetraacetate and Conjugation to Cysteine Side Chains

1997 • 63 citations

Amino Acid Residues in Both the Protein Splicing and Endonuclease Domains of the PI-SceI Intein Mediate DNA Binding

1998 • 55 citations

Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI

1996 • 54 citations

Mapping the Promoter DNA Sites Proximal to Conserved Regions of σ⁷⁰ in an Escherichia coli RNA Polymerase−lacUV5 Open Promoter Complex

1998 • 51 citations

[24] Characterization of protein—DNA complexes by affinity cleaving

1991 • 40 citations

The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding

1998 • 39 citations

1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA–Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays

2003 • 31 citations

Novel site-specific DNA endonucleases

1998 • 30 citations

Identification of Lys-403 in the PI-SceI Homing Endonuclease as Part of a Symmetric Catalytic Center

1998 • 29 citations

Photocross-linking of the Homing Endonuclease PI-SceI to Its Recognition Sequence

1999 • 28 citations

Mapping of a DNA Binding Region of the PI-SceI Homing Endonuclease by Affinity Cleavage and Alanine-Scanning Mutagenesis

1999 • 23 citations

Structure of the LexA Repressor−DNA Complex Probed by Affinity Cleavage and Affinity Photo-Cross-Linking

1996 • 21 citations

Phenyl-azide-mediated Photocrosslinking Analysis of Cro-DNA Interaction

1993 • 20 citations

Orientation of the LexA DNA-binding motif on operator DNA as inferred from cysteine-mediated phenyl azide crosslinking.

1993 • 18 citations