Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity.

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Biostatistical Analysis

1996 • 36,713 citations

Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor

1987 • 10,774 citations

Nitric oxide as a secretory product of mammalian cells

1992 • 4,437 citations

Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.

1990 • 3,304 citations

Passive Immunization Against Cachectin/Tumor Necrosis Factor Protects Mice from Lethal Effect of Endotoxin

1985 • 2,380 citations

Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase

1991 • 2,371 citations

Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse Macrophages

1992 • 1,914 citations

Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.

1986 • 1,439 citations

Glucocorticoids inhibit the expression of an inducible, but not the constitutive, nitric oxide synthase in vascular endothelial cells.

1990 • 1,117 citations

Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.

1991 • 982 citations

Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.

1992 • 877 citations

Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shock

1991 • 873 citations

Interleukin-1 receptor antagonist reduces mortality from endotoxin shock

1990 • 865 citations

Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes.

1993 • 845 citations

Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine.

1993 • 832 citations

Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein.

1991 • 812 citations

Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation

1983 • 801 citations

Tumor necrosis factor downregulates an endothelial nitric oxide synthase mRNA by shortening its half-life.

1993 • 778 citations

Analysis of reduced forms of biopterin in biological tissues and fluids

1980 • 713 citations

Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells

1990 • 700 citations

Macrophage Oxidation of L-Arginine to Nitric Oxide, Nitrite, and Nitrate

1989 • 681 citations

Cytokines, endotoxin, and glucocorticoids regulate the expression of inducible nitric oxide synthase in hepatocytes.

1993 • 671 citations

Calmodulin is a subunit of nitric oxide synthase from macrophages.

1992 • 640 citations

Nitric oxide synthase is a cytochrome P-450 type hemoprotein

1992 • 617 citations

Nitrogen Oxide Levels in Patients After Trauma and During Sepsis

1991 • 609 citations

Reduced Biopterin as a Cofactor in the Generation of Nitrogen Oxides by Murine Macrophages

1989 • 571 citations

Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle

1993 • 552 citations

BIOSYNTHESIS AND METABOLISM OF TETRAHYDROBIOPTERIN AND MOLYBDOPTERIN

1985 • 542 citations

Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo

1990 • 536 citations

Culture of vascular endothelium.

1976 • 532 citations

Calcium‐dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin

1990 • 507 citations

Molecular cloning and characterization of human endothelial nitric oxide synthase

1992 • 494 citations

Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase.

1992 • 477 citations

Endothelial Cell Production of Nitrogen Oxides in Response to Interferon in Combination With Tumor Necrosis Factor, Interleukin-1, or Endotoxin

1990 • 474 citations

Tetrahydrobiopterin synthesis. An absolute requirement for cytokine-induced nitric oxide generation by vascular smooth muscle.

1992 • 444 citations

Stimulation of the nitric oxide synthase pathway in human hepatocytes by cytokines and endotoxin.

1992 • 428 citations

Cytokine-activated endothelial cells express an isotype of nitric oxide synthase which is tetrahydrobiopterin-dependent, calmodulin-independent and inhibited by arginine analogs with a rank-order of potency characteristic of activated macrophages

1991 • 391 citations

Macrophage nitric oxide synthase subunits. Purification, characterization, and role of prosthetic groups and substrate in regulating their association into a dimeric enzyme.

1993 • 391 citations

Tumor Necrosis Factor and Granulocyte Macrophage-Colony Stimulating Factor Stimulate Human Macrophages to Restrict Growth of Virulent Mycobacterium avium and to Kill Avirulent M. avium: Killing Effector Mechanism Depends on the Generation of Reactive Nitrogen Intermediates

1991 • 390 citations

Cloning of Inducible Nitric Oxide Synthase in Rat Vascular Smooth Muscle Cells

1993 • 319 citations

The Role of Neopterin as a Monitor of Cellular Immune Activation in Transplantation, Inflammatory, Infectious, and Malignant Diseases

1992 • 318 citations

Evidence for IFN-gamma as a mediator of the lethality of endotoxin and tumor necrosis factor-alpha.

1992 • 300 citations

Tetrahydrobiopterin-dependent formation of nitrite and nitrate in murine fibroblasts.

1990 • 288 citations

Pteridine biosynthesis in human endothelial cells. Impact on nitric oxide-mediated formation of cyclic GMP.

1993 • 266 citations

Functional heterogeneity of vascular endothelial cells

1987 • 265 citations

L-citrulline production from L-arginine by macrophage nitric oxide synthase. The ureido oxygen derives from dioxygen.

1990 • 240 citations

Platelet adhesion to human vascular endothelium is modulated by constitutive and cytokine induced nitric oxide

1993 • 222 citations

Parallel induction of tetrahydrobiopterin biosynthesis and indoleamine 2,3-dioxygenase activity in human cells and cell lines by interferon-γ

1989 • 201 citations

Nitric oxide synthesis in endothelial cells: evidence for a pathway inducible by TNF-alpha

1991 • 200 citations

Regulation of muscle transcription by the MyoD family. The heart of the matter.

1993 • 174 citations

Macrophage nitric oxide synthase: relationship between enzyme-bound tetrahydrobiopterin and synthase activity

1992 • 174 citations

Tetrahydrobiopterin, a cofactor for rat cerebellar nitric oxide synthase, does not function as a reactant in the oxygenation of arginine.

1991 • 164 citations

Mutation of N-myristoylation site converts endothelial cell nitric oxide synthase from a membrane to a cytosolic protein.

1993 • 162 citations

Tetrahydrobiopterin-dependent formation of endothelium-derived relaxing factor (nitric oxide) in aortic endothelial cells

1992 • 162 citations

Hepatocytes and Macrophages Express an Identical Cytokine-Inducible Nitric Oxide Synthase Gene

1993 • 113 citations

Lectures on General Pathology

1954 • 102 citations

GTP Cyclohydrolase I mRNA Is Induced by LPS in Vascular Smooth Muscle: Characterization, Sequence and Relationship to Nitric Oxide Synthase

1993 • 102 citations

Cloning and sequencing of cDNA encoding rat GTP cyclohydrolase I. The first enzyme of the tetrahydrobiopterin biosynthetic pathway.

1991 • 97 citations

Tetrahydrobiopterin is required for cytokine-induced nitric oxide production in a murine macrophage cell line (RAW 264).

1993 • 95 citations

Induction of a macrophage-like nitric oxide synthase in cultured rat aortic endothelial cells. IL-1 beta-mediated induction regulated by tumor necrosis factor-alpha and IFN-gamma.

1993 • 92 citations

Effects of interferon-gamma on nitric oxide synthase activity and endothelin-1 production by vascular endothelial cells.

1992 • 84 citations

Relationship between neopterin and granulocyte elastase plasma levels and the severity of multiple organ failure

1989 • 84 citations

Guanosine triphosphate cyclohydrolase I assay in human and rat liver using high-performance liquid chromatography of neopterin phosphates and guanine nucleotides

1983 • 64 citations

Identification of inducible calmodulin-dependent nitric oxide synthase in the liver of rats.

1992 • 53 citations

Hyperphenylalaninemia Due to Dihydropteridine Reductase Deficiency: Diagnosis by Measurement of Oxidized and Reduced Pterins in Urine

1980 • 50 citations

Impact of tumour necrosis factor-α and interferon-γ on tetrahydrobiopterin synthesis in murine fibroblasts and macrophages

1991 • 48 citations

Biosynthesis and Metabolism of Tetrahydrobiopterin and Molybdopterin

1985 • 36 citations

Course of Immune Activation Markers in Patients after Severe Multiple Trauma

1990 • 7 citations