Lipid–protein interactions in blood coagulation

The coagulation cascade: initiation, maintenance, and regulation

1991 • 1,810 citations

Anti-phospholipid antibodies are directed against a complex antigen that includes a lipid-binding inhibitor of coagulation: beta 2-glycoprotein I (apolipoprotein H).

1990 • 1,618 citations

Pathophysiologic Implications of Membrane Phospholipid Asymmetry in Blood Cells

1997 • 1,321 citations

Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactor

1990 • 1,294 citations

The molecular basis of blood coagulation

1988 • 1,240 citations

ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes.

1984 • 692 citations

The role of phospholipids and factor Va in the prothrombinase complex.

1980 • 672 citations

Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity.

1988 • 663 citations

The antiphospholipid syndrome: ten years on

1993 • 640 citations

Assembly of the Platelet Prothrombinase Complex Is Linked to Vesiculation of the Platelet Plasma Membrane

1989 • 597 citations

The contribution of bovine Factor V and Factor Va to the activity of prothrombinase.

1979 • 592 citations

The role of phospholipid and factor VIIIa in the activation of bovine factor X.

1981 • 590 citations

Lupus Anticoagulant IgG's (LA) Are Not Directed to Phospholipids only, but to a Complex of Lipid-Bound Human Prothrombin

1991 • 583 citations

Changes in membrane phospholipid distribution during platelet activation

1983 • 551 citations

Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface.

1994 • 535 citations

Pregnancy Loss in the Antiphospholipid-Antibody Syndrome — A Possible Thrombogenic Mechanism

1997 • 525 citations

The inhibition of blood coagulation by activated protein C through the selective inactivation of activated factor V

1979 • 480 citations

Generation of Prothrombin‐Converting Activity and the Exposure of Phosphatidylserine at the Outer Surface of Platelets

1982 • 472 citations

Blood coagulation factors V and VIII: structural and functional similarities and their relationship to hemorrhagic and thrombotic disorders

1988 • 405 citations

Thrombomodulin as a model of molecular mechanisms that modulate protease specificity and function at the vessel surface

1995 • 380 citations

Binding of annexin V/placental anticoagulant protein I to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated platelets.

1990 • 372 citations

Autoantibodies to phospholipid-binding plasma proteins: a new view of lupus anticoagulants and other "antiphospholipid" autoantibodies [see comments]

1994 • 364 citations

"Anticardiolipin" autoantibodies recognize beta 2-glycoprotein I in the absence of phospholipid. Importance of Ag density and bivalent binding.

1995 • 337 citations

Complete amino acid sequence of human plasma beta 2-glycoprotein I.

1984 • 326 citations

Transbilayer movement of phospholipids in red cell and platelet membranes

1991 • 317 citations

Autoantibodies to phospholipid-binding plasma proteins: a new view of lupus anticoagulants and other "antiphospholipid" autoantibodies [see comments]

1994 • 317 citations

γ-carboxyglutamate-containing proteins and the vitamin K-dependent carboxylase

1990 • 312 citations

Membrane asymmetry and blood coagulation

1977 • 309 citations

Loss of membrane phospholipid asymmetry in platelets and red cells may be associated with calcium-induced shedding of plasma membrane and inhibition of aminophospholipid translocase

1990 • 290 citations

The protein C anticoagulant system: Inherited defects as basis for venous thrombosis

1995 • 286 citations

Activation of human prothrombin by human prothrombinase. Influence of factor Va on the reaction mechanism.

1987 • 278 citations

Role of gamma-carboxyglutamic acid. An unusual protein transition required for the calcium-dependent binding of prothrombin to phospholipid.

1976 • 278 citations

Comparison of anticoagulant and procoagulant activities of stimulated platelets and platelet-derived microparticles

1991 • 277 citations

Platelet-derived microparticles express high affinity receptors for factor VIII.

1991 • 277 citations

The fifth domain of beta 2-glycoprotein I contains a phospholipid binding site (Cys281-Cys288) and a region recognized by anticardiolipin antibodies.

1994 • 275 citations

A Phospholipid-β2-Glycoprotein I Complex Is an Antigen for Anticardiolipin Antibodies Occurring in Autoimmune Disease But Not with Infection

1992 • 262 citations

The calcium ion and membrane binding structure of the Gla domain of calcium-prothrombin fragment 1

1992 • 259 citations

Factor VII binding to tissue factor in reconstituted phospholipid vesicles: induction of cooperativity by phosphatidylserine

1986 • 259 citations

The role of activated human platelets in prothrombin and factor X activation

1985 • 240 citations

Differentiation of metal ion-induced transitions of prothrombin fragment 1.

1977 • 235 citations

Membrane and lipid involvement in blood coagulation

1978 • 234 citations

Molecular Events that Control the Protein C Anticoagulant Pathway

1993 • 223 citations

Maintenance and consequences of membrane phospholipid asymmetry

1994 • 218 citations

The tissue factor pathway of blood coagulation.

1992 • 212 citations

Lupus anticoagulant activity of autoimmune antiphospholipid antibodies is dependent upon beta 2-glycoprotein I.

1992 • 206 citations

Role of Divalency in the High-Affinity Binding of Anticardiolipin Antibody−β₂-Glycoprotein I Complexes to Lipid Membranes

1996 • 201 citations

A Factor VIII Neutralizing Monoclonal Antibody and a Human Inhibitor Alloantibody Recognizing Epitopes in the C2 Domain Inhibit Factor VIII Binding to von Willebrand Factor and to Phosphatidylserine

1993 • 199 citations

Synthetic factor VIII peptides with amino acid sequences contained within the C2 domain of factor VIII inhibit factor VIII binding to phosphatidylserine

1990 • 198 citations

Scott syndrome, characterized by impaired transmembrane migration of procoagulant phosphatidylserine and hemorrhagic complications, is an inherited disorder

1996 • 192 citations

Complete nucleotide and deduced amino acid sequence of human β2-glycoprotein I

1991 • 187 citations

Anticoagulant Activity of β2-Glycoprotein I Is Potentiated by a Distinct Subgroup of Anticardiolipin Antibodies

1992 • 183 citations

The intrinsic pKa values for phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine in monolayers deposited on mercury electrodes

1994 • 180 citations

Continuous Analysis of the Mechanism of Activated Transbilayer Lipid Movement in Platelets

1995 • 179 citations

Phosphatidylethanolamine incorporation into vesicles selectively enhances factor Va inactivation by activated protein C.

1994 • 177 citations

Lupus anticoagulant activity is frequently dependent on the presence of beta 2-glycoprotein I.

1992 • 175 citations

The interaction of bovine factor V and factor V-derived peptides with phospholipid vesicles.

1983 • 171 citations

The active site of blood coagulation factor Xa. Its distance from the phospholipid surface and its conformational sensitivity to components of the prothrombinase complex.

1987 • 170 citations

Surface-dependent hemostasis.

1992 • 167 citations

Platelet procoagulant activity and microvesicle formation. Its putative role in hemostasis and thrombosis.

1992 • 166 citations

Calcium involvement in aminophospholipid exposure and microparticle formation during platelet activation: A study using Ca2+-ATPase inhibitors

1995 • 163 citations

Bidirectional transbilayer movement of phospholipid analogs in human red blood cells. Evidence for an ATP-dependent and protein-mediated process.

1992 • 160 citations

Prothrombinase complex assembly. Kinetic mechanism of enzyme assembly on phospholipid vesicles.

1988 • 158 citations

Kinetics of factor IX activation via the extrinsic pathway. Dependence of Km on tissue factor.

1980 • 157 citations

Role of gamma-carboxyglutamic acid. Cation specificity of prothrombin and factor X-phospholipid binding.

1976 • 152 citations

The prothrombinase-catalyzed activation of prothrombin proceeds through the intermediate meizothrombin in an ordered, sequential reaction.

1986 • 151 citations

The Last Three Consecutive Epidermal Growth Factor-like Structures of Human Thrombomodulin Comprise the Minimum Functional Domain for Protein C-activating Cofactor Activity and Anticoagulant Activity

1989 • 149 citations

Structure of the Ca2+-free GLA domain sheds light on membrane binding of blood coagulation proteins

1995 • 144 citations

Lupus Anticoagulant Activity Is Frequently Dependent on the Presence of β2-Glycoprotein I

1992 • 142 citations

Defective Ca(2+)-induced microvesiculation and deficient expression of procoagulant activity in erythrocytes from a patient with a bleeding disorder: a study of the red blood cells of Scott syndrome

1992 • 142 citations

Role of Translocases in the Generation of Phosphatidylserine Asymmetry

1996 • 141 citations

Cell Mediated Events that Control Blood Coagulation and Vascular Injury

1993 • 138 citations

Impaired factor X and prothrombin activation associated with decreased phospholipid exposure in platelets from a patient with a bleeding disorder

1985 • 135 citations

Human beta2-glycoprotein I as an anticardiolipin cofactor determined using mutants expressed by a baculovirus system

1996 • 132 citations

Identification of the Phospholipid Binding Site in the Vitamin K-dependent Blood Coagulation Protein Factor IX

1996 • 131 citations

The kinetics of activation of normal and gamma-carboxyglutamic acid-deficient prothrombins.

1985 • 131 citations

The binding of factor Va to phospholipid vesicles.

1988 • 125 citations

Prothrombin requires two sequential metal-dependent conformational transitions to bind phospholipid. Conformation-specific antibodies directed against the phospholipid-binding site on prothrombin.

1986 • 124 citations

Deletion analysis of recombinant human factor V. Evidence for a phosphatidylserine binding site in the second C-type domain.

1992 • 115 citations

Phosphatidylethanolamine augments factor VIIa-tissue factor activity: enhancement of sensitivity to phosphatidylserine

1995 • 114 citations

Factor VII autoactivation proceeds via interaction of distinct protease-cofactor and zymogen-cofactor complexes. Implications of a two-dimensional enzyme kinetic mechanism.

1993 • 112 citations

Formation of meizothrombin as intermediate in factor Xa-catalyzed prothrombin activation.

1986 • 111 citations

Membrane-dependent reactions in blood coagulation: role of the vitamin K-dependent enzyme complexes

1994 • 110 citations

Calcium-induced transbilayer scrambling of fluorescent phospholipid analogs in platelets and erythrocytes

1994 • 110 citations

Structure of the prothrombin- and blood clotting factor X-membrane complexes

1977 • 108 citations

Tissue factor and its extracellular soluble domain: the relationship between intermolecular association with factor VIIa and enzymic activity of the complex

1992 • 108 citations

The binding of 35S-labeled recombinant factor VIII to activated and unactivated human platelets.

1988 • 107 citations

Antiphosphatidylserine antibody removes annexin-V and facilitates the binding of prothrombin at the surface of a choriocarcinoma model of trophoblast differentiation

1997 • 104 citations

Comparison of Naturally Occurring Vitamin K-Dependent Proteins: Correlation of Amino Acid Sequences and Membrane Binding Properties Suggests a Membrane Contact Site

1997 • 104 citations

Proteolytic formation and properties of functional domains of thrombomodulin.

1987 • 98 citations

Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine

1993 • 94 citations

Kinetic studies of prothrombin activation: effect of factor Va and phospholipids on formation of the enzyme-substrate complex

1984 • 93 citations

Activation of the Factor VIIIa-Factor IXa Enzyme Complex of Blood Coagulation by Membranes Containing Phosphatidyl-L-serine

1996 • 89 citations

Effect of antiphospholipid antibodies on procoagulant activity of activated platelets and platelet‐derived microvesicles

1993 • 89 citations

Structure of calcium prothrombin fragment 1 including the conformation of the Gla domain

1989 • 88 citations

Beta2-Glycoprotein I

1994 • 87 citations

Structure of the Calcium Ion-Bound .gamma.-Carboxyglutamic Acid-Rich Domain of Factor IX

1995 • 87 citations

Effector Cell Protease Receptor-1, a Platelet Activation-dependent Membrane Protein, Regulates Prothrombinase-catalyzed Thrombin Generation

1997 • 87 citations

The association of bovine prothrombin fragment 1 with phospholipid. Quantitative characterization of the Ca2+ ion-mediated binding of prothrombin fragment 1 to phospholipid vesicles and a molecular model for its association with phospholipids.

1979 • 86 citations

Identification of Structural Mutations in the Fifth Domain of Apolipoprotein H ( 2-Glycoprotein I) Which Affect Phospholipid Binding

1997 • 86 citations

Protein Structural Requirements and Properties of Membrane Binding by γ-Carboxyglutamic Acid-containing Plasma Proteins and Peptides

1989 • 86 citations

Interaction of bovine blood clotting factor Va and its subunits with phospholipid vesicles

1983 • 85 citations

Prothrombin activation on phospholipid membranes with positive electrostatic potential

1988 • 77 citations

The Location of the Active Site of Blood Coagulation Factor VIIa above the Membrane Surface and Its Reorientation upon Association with Tissue Factor

1996 • 72 citations

Assembly of the Prothrombinase Complex on Lipid Vesicles Depends on the Stereochemical Configuration of the Polar Headgroup of Phosphatidylserine

1994 • 72 citations

The activation of prothrombin by the prothrombinase complex. The contribution of the substrate-membrane interaction to catalysis.

1994 • 72 citations

Meizothrombin formation during factor Xa-catalyzed prothrombin activation. Formation in a purified system and in plasma.

1991 • 70 citations

Changes in β2-Glycoprotein I Antigenicity Induced by Phospholipid Binding

1993 • 67 citations

In Vivo Models of Thrombosis for the Antiphospholipid Syndrome

1996 • 66 citations

Contribution of different phospholipid classes to the prothrombin converting capacity of sonicated lipid vesicles

1996 • 62 citations

Membrane-binding peptide from the C2 domain of factor VIII forms an amphipathic structure as determined by NMR spectroscopy

1995 • 61 citations

Production and characterization of transformed B-lymphocytes expressing the membrane defect of Scott syndrome.

1994 • 60 citations

Phosphatidylethanolamine Induces High Affinity Binding Sites for Factor VIII on Membranes Containing Phosphatidyl-L-serine

1995 • 59 citations

The factor IX phospholipid-binding site is required for calcium-dependent activation of factor IX by factor XIa.

1987 • 59 citations

Factor Va-membrane interaction is mediated by two regions located on the light chain of the cofactor

1994 • 56 citations

Amino-terminal alanine functions in a calcium-specific process essential for membrane binding by prothrombin fragment 1

1988 • 56 citations

Structure of the Metal-free γ-Carboxyglutamic Acid-rich Membrane Binding Region of Factor IX by Two-dimensional NMR Spectroscopy

1995 • 55 citations

The binding energy of human coagulation protein C to acidic phospholipid vesicles contains a major contribution from leucine 5 in the gamma-carboxyglutamic acid domain.

1994 • 54 citations

Identification and characterization of a phospholipid-binding site of bovine factor Va.

1990 • 50 citations

Soluble Phospholipids Enhance Factor X_a-Catalyzed Prothrombin Activation in Solution

1996 • 50 citations

Factor VIIa-Tissue Factor: Functional Importance of Protein-Membrane Interactions

1997 • 50 citations

Specific contribution of different phospholipid surfaces to the activation of prothrombin by the fully assembled prothrombinase.

1993 • 49 citations

Role of the N- and C-Terminal Domains of Bovine β2-Glycoprotein I in Its Interaction with Cardiolipin1

1995 • 46 citations

Phospholipid-Specific Conformational Changes in Human Prothrombin upon Binding to Procoagulant Acidic Lipid Membranes

1994 • 46 citations

Membrane binding properties of blood coagulation factor V and derived peptides

1984 • 46 citations

Non β₂-Glycoprotein I Cofactors for Antiphospholipid Antibodies

1996 • 45 citations

Lipid mobility in the assembly and expression of the activity of the prothrombinase complex.

1985 • 42 citations

Prothrombin activation on membranes with anionic lipids containing phosphate, sulfate and/or carboxyl groups

1990 • 42 citations

Hydrophobic Amino Acid Residues of Human Anticoagulation Protein C That Contribute to Its Functional Binding to Phospholipid Vesicles

1995 • 42 citations

Increased aminophospholipid translocase activity in human platelets during secretion

1990 • 41 citations

A domain of membrane-bound blood coagulation factor Va is located far from the phospholipid surface. A fluorescence energy transfer measurement

1986 • 40 citations

Thrombogenic Mechanisms of Antiphospholipid Antibodies

1997 • 38 citations

Binding of bovine factor Va to phosphatidylcholine membranes

1996 • 38 citations

Electrostatic and hydrophobic interactions are involved in factor Va binding to membranes containing acidic phospholipids.

1994 • 38 citations

Effect of membrane fluidity and fatty acid composition on the prothrombin-converting activity of phospholipid vesicles

1992 • 37 citations

Prothrombin-membrane interaction. Effects of ionic strength, pH, and temperature

1980 • 36 citations

Structure of membrane‐bound human factor Va

1994 • 35 citations

Enhancement of thrombin-thrombomodulin-catalysed protein C activation by phosphatidylethanolamine containing unsaturated fatty acids: possible physiological significance of phosphatidylethanolamine in anticoagulant activity of thrombomodulin

1994 • 32 citations

[1] Modular design of proteases of coagulation, fibrinolysis, and complement activation: Implications for protein engineering and structure-function studies

1993 • 31 citations

A membrane-mediated catalytic event in prothrombin activation.

1994 • 31 citations

Binding of blood coagulation factor VIII and its light chain to phosphatidylserine/phosphatidylcholine bilayers as measured by ellipsometry

1995 • 29 citations

Structurally and Functionally Distinct Ca²⁺ Binding Sites in the γ‐Carboxyglutamic Acid‐Containing Domain of Factor VIIa

1995 • 27 citations

Fluorescence Resonance Energy Transfer Study of Shape Changes in Membrane-Bound Bovine Prothrombin and Meizothrombin

1997 • 27 citations

Inhibition of Phospholipid-Dependent Coagulation Reactions by 'Antiphospholipid Antibodies': Possible Modes of Action

1994 • 26 citations

Insights into the complex association of bovine factor Va with acidic-lipid-containing synthetic membranes

1996 • 25 citations

The association of human coagulation factors VIII, IXa and X with phospholipid vesicles involves both electrostatic and hydrophobic interactions

1992 • 23 citations

Antibodies Against Platelet-Activating Factor in Patients with Antiphospholipid Antibodies

1994 • 20 citations

Phosphatidylserine-Containing Membranes Alter the Thermal Stability of Prothrombin's Catalytic Domain: A Differential Scanning Calorimetric Study

1994 • 19 citations

Tissue factor

1995 • 19 citations

Prothrombin activation on dioleoylphosphatidylcholine membranes

1994 • 19 citations

Calcium-independent activation of prothrombin on membranes with positively charged lipids

1988 • 18 citations

The metal‐dependent conformational changes in Factor IX associated with phospholipid binding.

1993 • 16 citations

Modification of the N-terminus of human factor IX by defective propeptide cleavage or acetylation results in a destabilized calcium-induced conformation: effects on phospholipid binding and activation by factor XIa

1997 • 10 citations

Properties of a Recombinant Chimeric Protein in which the gamma-Carboxyglutamic Acid and Helical Stack Domains of Human Anticoagulant Protein C Are Replaced by those of Human Coagulation Factor VII

1997 • 9 citations