Molecular Events that Control the Protein C Anticoagulant Pathway

The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

1989 • 889 citations

Factor XI Activation in a Revised Model of Blood Coagulation

1991 • 774 citations

The refined 1.9‐Å X‐ray crystal structure of d‐Phe‐Pro‐Arg chloromethylketone‐inhibited human α‐thrombin: Structure analysis, overall structure, electrostatic properties, detailed active‐site geometry, and structure‐function relationships

1992 • 628 citations

Human Plasma Protein C

1979 • 511 citations

Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.

1987 • 358 citations

Key residues involved in calcium-binding motifs in EGF-like domains

1991 • 282 citations

Phospholipid-independent and -dependent interactions required for tissue factor receptor and cofactor function

1991 • 228 citations

The interaction of thrombin with fibrinogen

1992 • 216 citations

Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene

1987 • 209 citations

Proteolytic formation and properties of gamma-carboxyglutamic acid-domainless protein C.

1983 • 202 citations

Regulation of blood coagulation by the protein C system

1992 • 179 citations

The active site of blood coagulation factor Xa. Its distance from the phospholipid surface and its conformational sensitivity to components of the prothrombinase complex.

1987 • 170 citations

How an epidermal growth factor (EGF)-like domain binds calcium. High resolution NMR structure of the calcium form of the NH2-terminal EGF-like domain in coagulation factor X.

1992 • 166 citations

Structure-function relationships of epidermal growth factor modules in vitamin K-dependent clotting factors

1991 • 157 citations

The interaction of a Ca2+-dependent monoclonal antibody with the protein C activation peptide region. Evidence for obligatory Ca2+ binding to both antigen and antibody.

1988 • 156 citations

Steady state kinetic parameters for the thrombin-catalyzed conversion of human fibrinogen to fibrin.

1983 • 154 citations

Hereditary deficiency of antithrombin III, protein C and protein S: prevalence in patients with a history of venous thrombosis and criteria for rational patient screening.

1992 • 151 citations

The Last Three Consecutive Epidermal Growth Factor-like Structures of Human Thrombomodulin Comprise the Minimum Functional Domain for Protein C-activating Cofactor Activity and Anticoagulant Activity

1989 • 149 citations

Glu-192----Gln substitution in thrombin mimics the catalytic switch induced by thrombomodulin.

1991 • 141 citations

The active site of thrombin is altered upon binding to thrombomodulin. Two distinct structural changes are detected by fluorescence, but only one correlates with protein C activation.

1991 • 136 citations

Loss of prothrombin and of factor Xa-factor Va interactions upon inactivation of factor Va by activated protein C.

1984 • 133 citations

A 10-kDa cyanogen bromide fragment from the epidermal growth factor homology domain of rabbit thrombomodulin contains the primary thrombin binding site.

1988 • 130 citations

Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.

1987 • 126 citations

Stable expression of a secretable deletion mutant of recombinant human thrombomodulin in mammalian cells.

1990 • 117 citations

The function of calcium in protein C activation by thrombin and the thrombin-thrombomodulin complex can be distinguished by mutational analysis of protein C derivatives.

1992 • 114 citations

Calcium binding to the isolated β-hydroxyaspartic acid-containing epidermal growth factor-like domain of bovine factor X

1989 • 109 citations

The effect of bovine thrombomodulin on the specificity of bovine thrombin.

1986 • 106 citations

The active site of factor IXa is located far above the membrane surface and its conformation is altered upon association with factor VIIIa. A fluorescence study.

1992 • 105 citations

Assembly of the prothrombinase complex in the absence of prothrombin.

1981 • 104 citations

Calcium binding to the epidermal growth factor homology region of bovine protein C.

1988 • 99 citations

Isolation and characterization of the glycosaminoglycan component of rabbit thrombomodulin proteoglycan.

1990 • 99 citations

Reconstitution of rabbit thrombomodulin into phospholipid vesicles.

1987 • 99 citations

Proteolytic formation and properties of functional domains of thrombomodulin.

1987 • 98 citations

Beta-hydroxyaspartic acid in the first epidermal growth factor-like domain of protein C. Its role in Ca2+ binding and biological activity.

1988 • 98 citations

The fifth and sixth growth factor-like domains of thrombomodulin bind to the anion-binding exosite of thrombin and alter its specificity.

1992 • 97 citations

Calcium-binding properties of bovine factor X lacking the gamma-carboxyglutamic acid-containing region.

1984 • 96 citations

Thrombin Glu-39 restricts the P'3 specificity to nonacidic residues

1991 • 90 citations

Structure of the hirulog 3-thrombin complex and nature of the S' subsites of substrates and inhibitors

1992 • 88 citations

Interaction of calcium with bovine plasma protein C

1981 • 88 citations

Equilibrium binding of thrombin to recombinant human thrombomodulin: effect of hirudin, fibrinogen, factor Va, and peptide analogs

1990 • 88 citations

Microthrombomodulin

1989 • 86 citations

Relationship between anticoagulant activities and polyanionic properties of rabbit thrombomodulin.

1988 • 81 citations

Further localization of binding sites for thrombin and protein C in human thrombomodulin

1990 • 80 citations

Interaction of clotting factor V heavy chain with prothrombin and prethrombin 1 and role of activated protein C in regulating this interaction: analysis by analytical ultracentrifugation

1989 • 71 citations

Binding of thrombin to thrombomodulin accelerates inhibition of the enzyme by antithrombin III. Evidence for a heparin-independent mechanism

1987 • 70 citations

Calcium-dependent interaction between the epidermal growth factor precursor-like region of human protein C and a monoclonal antibody.

1987 • 65 citations

Interaction of thrombin des-ETW with antithrombin III, the Kunitz inhibitors, thrombomodulin and protein C. Structural link between the autolysis loop and the Tyr-Pro-Pro-Trp insertion of thrombin.

1992 • 63 citations

Protein structural requirements for Ca2+ binding to the light chain of factor X. Studies using isolated intact fragments containing the gamma-carboxyglutamic acid region and/or the epidermal growth factor-like domains

1991 • 62 citations

The amino‐terminal domain of thrombomodulin and pancreatic stone protein are homologous with lectins

1988 • 61 citations

Analysis of the functions of the first epidermal growth factor-like domain of factor X.

1993 • 59 citations

Characterization of the functional defect in factor IX Alabama. Evidence for a conformational change due to high affinity calcium binding in the first epidermal growth factor domain.

1990 • 59 citations

The chondroitin sulfate moiety of thrombomodulin binds a second molecule of thrombin.

1993 • 58 citations

Heparin binding site, conformational change, and activation of antithrombin

1992 • 58 citations

The active site of membrane-bound meizothrombin. A fluorescence determination of its distance from the phospholipid surface and its conformational sensitivity to calcium and factor Va.

1990 • 50 citations

Expression of human soluble tissue factor in yeast and enzymatic properties of its complex with factor VIIa.

1992 • 50 citations

Enhancing protein C interaction with thrombin results in a clot-activated anticoagulant

1992 • 49 citations

Recombinant human protein C derivatives: altered response to calcium resulting in enhanced activation by thrombin.

1990 • 48 citations

Three-dimensional structure of the apo form of the N-terminal EGF-like module of blood coagulation factor X as determined by NMR spectroscopy and simulated folding

1992 • 45 citations

Aspartic acid 349 in the fourth epidermal growth factor-like structure of human thrombomodulin plays a role in its Ca(2+)-mediated binding to protein C.

1991 • 44 citations

Evidence for multiple conformational changes in the active center of thrombin induced by complex formation with thrombomodulin: an analysis employing nitroxide spin-labels

1988 • 42 citations

Thrombomodulin

1991 • 38 citations

Localization of thrombomodulin-binding site within human thrombin.

1990 • 37 citations

Recombinant human thrombomodulin. Regulation of cofactor activity and anticoagulant function by a glycosaminoglycan side chain

1992 • 36 citations

Structure-function studies of the epidermal growth factor domains of human thrombomodulin

1992 • 33 citations

Calcium-dependence of the interactions between protein C, thrombin and the elastase fragment of thrombomodulin. Analysis by ultracentrifugation

1992 • 29 citations

The high affinity calcium-binding site involved in protein C activation is outside the first epidermal growth factor homology domain.

1992 • 29 citations

Biological relevance of the protein C system and laboratory diagnosis of protein C and protein S deficiencies

1990 • 26 citations

Effect of rabbit thrombomodulin on thrombin inhibition by antithrombin in the presence of heparin

1989 • 25 citations

Identification of structural domains in protein C involved in its interaction with thrombin-thrombomodulin on the surface of endothelial cells.

1992 • 25 citations

10 Protein C and protein S

1989 • 24 citations

Thrombomodulin: An Anticoagulant Cell Surface Proteoglycan With Physiologically Relevant Glycosaminoglycan Moiety

1992 • 23 citations

Laboratory and clinical aspects of inherited thrombotic disorders

1992 • 17 citations

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