Mapping of viral epitopes with prokaryotic expression products

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

T-cell antigen receptor genes and T-cell recognition

1988 • 3,099 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation

1981 • 1,603 citations

Structure of a human common cold virus and functional relationship to other picornaviruses

1985 • 1,419 citations

The Antigenic Structure of Proteins: A Reappraisal

1984 • 957 citations

Strategies for epitope analysis using peptide synthesis

1987 • 887 citations

The three-dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution

1989 • 854 citations

The purification of eukaryotic polypeptides synthesized in Escherichia coli

1986 • 838 citations

Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis

1988 • 838 citations

Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli

1983 • 810 citations

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1988 • 662 citations

A sequence pattern common to T cell epitopes.

1988 • 623 citations

Correlation between segmental mobility and the location of antigenic determinants in proteins

1984 • 590 citations

Easy identification of cDNA clones.

1983 • 587 citations

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

1988 • 539 citations

Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

1984 • 538 citations

Human immunodeficiency virus type 1 neutralization epitope with conserved architecture elicits early type-specific antibodies in experimentally infected chimpanzees.

1988 • 514 citations

Intrinsic and Extrinsic Factors in Protein Antigenic Structure

1985 • 481 citations

Protein Folding Intermediates and Inclusion Body Formation.

1989 • 423 citations

Antigenicity: Some Molecular Aspects

1969 • 418 citations

Characterization of a human immunodeficiency virus neutralizing monoclonal antibody and mapping of the neutralizing epitope

1988 • 412 citations

‘ATG vectors’ for regulated high-level expression of cloned genes in Escherichia coli

1985 • 392 citations

[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

1987 • 392 citations

HTLV-III/LAV-Neutralizing Antibodies to anE. coli-Produced Fragment of the Virus Envelope

1986 • 336 citations

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

1985 • 331 citations

Efficient mapping of protein antigenic determinants.

1986 • 292 citations

Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins

1986 • 266 citations

Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus

1989 • 250 citations

Antigenic structures of proteins. Their determination has revealed important aspects of immune recognition and generated strategies for synthetic mimicking of protein binding sites

1984 • 245 citations

Fine mapping of an immunodominant domain in the transmembrane glycoprotein of human immunodeficiency virus

1987 • 242 citations

Three-dimensional structure of poliovirus serotype 1 neutralizing determinants

1988 • 225 citations

Structural and functional approaches to the study of protein antigenicity

1989 • 212 citations

[6] The use of pKC30 and its derivatives for controlled expression of genes

1983 • 202 citations

The Atomic Mobility Component of Protein Antigenicity

1985 • 201 citations

Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli

1985 • 174 citations

Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with Western blotting

1986 • 169 citations

Localization of epitopes of herpes simplex virus type 1 glycoprotein D

1985 • 164 citations

Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.

1984 • 159 citations

Mapping of T cell epitopes using recombinant antigens and synthetic peptides.

1987 • 152 citations

Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals.

1986 • 125 citations

A conserved region at the COOH terminus of human immunodeficiency virus gp120 envelope protein contains an immunodominant epitope.

1987 • 119 citations

Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus

1989 • 119 citations

Efficient mapping and characterization of a T cell epitope by the simultaneous synthesis of multiple peptides

1989 • 114 citations

pUEX, a bacterial expression vector related to pEX with universal host specificity

1987 • 113 citations

Use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the G2 glycoprotein of Rift Valley fever virus

1986 • 111 citations

Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

1983 • 108 citations

Secretion of heterologous gene products to the culture medium ofEscherichia coli

1986 • 103 citations

Solubilization and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants

1983 • 102 citations

Topological mapping of complement component C9 by recombinant DNA techniques suggests a novel mechanism for its insertion into target membranes.

1987 • 101 citations

Immunochemistry of viruses: The basis for serodiagnosis and vaccines

1986 • 95 citations

Neutralizing Epitopes of Type O Foot-and-Mouth Disease Virus. II. Mapping Three Conformational Sites with Synthetic Peptide Reagents

1989 • 92 citations

Synthesis of the complete trans-activation gene product of human T-lymphotropic virus type III in Escherichia coli: demonstration of immunogenicity in vivo and expression in vitro.

1986 • 90 citations

Epitope mapping of the human immunodeficiency virus type 1 gp120 with monoclonal antibodies

1988 • 83 citations

Specificity and Function of the Individual Amino Acids of an Important Determinant of Human Immunodeficiency Virus Type 1 that Induces Neutralizing Activity

1989 • 82 citations

HTLV-III env gene products synthesized in E. coli are recognized by antibodies present in the sera of AIDS patients

1985 • 81 citations

B- and T-lymphocyte responses to an immunodominant epitope of human immunodeficiency virus

1988 • 79 citations

Expression of Measles Virus Nucleoprotein in Escherichia coli: Use of Deletion Mutants to Locate the Antigenic Sites

1989 • 77 citations

[69] Expression, identification, and characterization of recombinant gene products in Escherichia coli

1987 • 76 citations

Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

1983 • 75 citations

Analysis of an immunodominant region of infectious bronchitis virus.

1989 • 73 citations

Antigenicity of the peplomer protein of infectious bronchitis virus

1989 • 73 citations

A poliovirus type 1 neutralization epitope is located within amino acid residues 93 to 104 of viral capsid polypeptide VP1.

1983 • 71 citations

Method to map antigenic determinants recognized by monoclonal antibodies: localization of a determinant of virus neutralization on the feline leukemia virus envelope protein gp70.

1984 • 69 citations

Construction of an excretion vector and extracellular production of human growth hormone from Escherichia coli

1987 • 68 citations

Antigenic cross-reactivity between proteins and peptides: new insights and applications

1987 • 68 citations

High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein

1983 • 67 citations

Analysis of HPV-1 E4 gene expression using epitope-defined antibodies.

1988 • 65 citations

Expression of the PDGF-related transforming protein of simian sarcoma virus in E. coli

1984 • 65 citations

A broad-host-range vector system for cloning and translational lacZ fusion analysis

1988 • 65 citations

A Neutralizing Epitope on Human Rhinovirus Type 2 Includes Amino Acid Residues between 153 and 164 of Virus Capsid Protein VP2

1987 • 64 citations

Expression in Escherichia Coli of an Epitope of the Glycoprotein of Infectious Hematopoietic Necrosis Virus Protects Against Viral Challenge

1988 • 63 citations

Gene cloning: An introduction

1991 • 62 citations

Protective immunisation against hepatitis B with an internal antigen of the virus

1987 • 61 citations

Peptides and the cellular immune response

1986 • 61 citations

Antigenicity and Immunogenicity of Synthetic Peptides of Foot-and-Mouth Disease Virus

1987 • 60 citations

Immunoenzymatic detection of expressed gene fragments cloned in the lac Z gene of E. coli.

1982 • 59 citations

Gene cloning: an introduction.

1990 • 58 citations

Human antibodies react with an epitope of the human papillomavirus type 6b L1 open reading frame which is distinct from the type-common epitope

1989 • 58 citations

Fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein D

1984 • 57 citations

Amino acid sequence of a conserved neutralizing epitope of murine coronaviruses

1989 • 56 citations

[31] Expression and secretion of foreign proteins in Escherichia coli

1987 • 56 citations

Analysis of functional domains on reovirus cell attachment protein σ1 using cloned s1 gene deletion mutants

1987 • 55 citations

Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC

1985 • 52 citations

Characterization and epitope mapping of a human monoclonal antibody reactive with the envelope glycoprotein of human immunodeficiency virus.

1987 • 51 citations

Serodiagnosis of Antibodies to the Human AIDS Retrovirus with a Bacterially Synthesized env Polypeptide

1986 • 44 citations

Bacterially expressed antigenic peptide from foot-and-mouth disease virus capsid elicits variable immunologic responses in animals.

1986 • 43 citations

A plasmid vector for cloning and expression of gene segments: expression of an HTLV-I envelope gene segment

1986 • 42 citations

Construction of a plasmid for expression of foreign epitopes as fusion proteins with subunit B of Escherichia coli heat-labile enterotoxin

1989 • 42 citations

Location of a Neutralizing Epitope for the Haemagglutinin-Neuraminidase Glycoprotein of Newcastle Disease Virus

1988 • 41 citations

Expression of a major bovine rotavirus neutralisation antigen (VP7c) in Escherichia coli

1987 • 40 citations

Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

1982 • 40 citations

Expression in Escherichia coli of cDNA fragments encoding the gene for the host-protective antigen of infectious bursal disease virus

1986 • 39 citations

Cloning vectors. A laboratory manual

1987 • 37 citations

Semliki Forest virus E2 envelope epitopes induce a nonneutralizing humoral response which protects mice against lethal challenge

1989 • 37 citations

Mapping of functional and antigenic domains of the alpha 4 protein of herpes simplex virus 1

1988 • 33 citations

Comparison of Western blot (immunoblot) based on recombinant-derived p41 with conventional tests for serodiagnosis of human immunodeficiency virus infections

1988 • 30 citations

An Antigenic Analysis of the Adenovirus Type 2 Fibre Polypeptide

1988 • 30 citations

Synthesis of fusion proteins with multiple copies of an antigenic determinant of foot-and-mouth disease virus antibodies

1986 • 30 citations

New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli

1989 • 29 citations

A plasmid expression vector that permits stabilization of both mRNAs and proteins encoded by the cloned genes

1986 • 28 citations

Mapping of antigenic domains of Sendai virus nucleocapsid protein expressed in Escherichia coli

1988 • 27 citations

Synthesis of the outer-capsid glycoprotein of the simian rotavirus SA11 in Escherichia coli

1986 • 27 citations

[30] Expression plasmid containing the λ PL promoter and cI857 repressor

1987 • 26 citations

High-level expression in Escherichia coli of a chemically synthesized gene for [Leu-28]echistatin

1989 • 26 citations

Use of simian virus 40 large T-beta-galactosidase fusion proteins in an immunochemical analysis of simian virus 40 large T antigen

1985 • 25 citations

Characterization of immunoreactive epitopes of the HIV-1 p41 envelope protein using fusion proteins synthesized in Escherichia coli

1988 • 24 citations

High-level expression vectors to synthesize unfused proteins in Escherichia coli

1986 • 24 citations

Recombinant Proteins: Virtual Authenticity

1987 • 23 citations

Improvement of the cloning linker of the bacterial expression vector pEX

1989 • 21 citations

λgt22, an improved λ vector for the directional cloning of full-length cDNA

1987 • 21 citations

Human retroviral env and gag polypeptides: serologic assays to measure infection.

1986 • 20 citations

Synthesis of fusion proteins containing antigenic determinants of foot-and-mouth disease virus

1986 • 20 citations

The Type-specific Epitopes of the Epstein-Barr Virus Nuclear Antigen 2 Are Near the Carboxy Terminus of the Protein

1989 • 18 citations

Identification of two epitopes in the carboxyterminal 15 amino acids of the E1 glycoprotein of mouse hepatitis virus A59 by using hybrid proteins

1986 • 18 citations

[25] Expression of eukaryotic genes in Escherichia coli with a synthetic two-cistron system

1987 • 17 citations

A versatile phage lambda expression vector system for cloning in Escherichia coli

1989 • 17 citations

Expression of Bovine Rotavirus Neutralization Antigen in Escherichia coli

1987 • 16 citations

Recombinant Polypeptides from the Human Immunodeficiency Virus Reverse Transcriptase Define Three Epitopes Recognized by Antibodies in Sera from Patients with Acquired Immunodeficiency Syndrome

1989 • 15 citations

Regions of poliovirus protein VP1 produced in Escherichia coli induce neutralizing antibodies

1987 • 13 citations

Conserved Immunogenic Region of a Major Core Protein (p24) of Human and Simian Immunodeficiency Viruses

1988 • 13 citations

High-level bacterial expression and purification of human T-lymphotropic virus type I (HTLV-I) transmembrane env protein

1985 • 13 citations

Expression in Escherichia coli of the major outer capsid protein of infectious pancreatic necrosis virus

1989 • 13 citations

Functional mutants of phenylalanine transfer RNA from Escherichia coli.

1985 • 12 citations

Identification of a DTH-inducing T-cell epitope on the E2 membrane protein of Semliki Forest virus

1989 • 12 citations

Use of TrpE/Gag fusion proteins to characterize immunoreactive domains on the human immunodeficiency virus type 1 core protein

1989 • 12 citations

Linear Neutralizing Epitopes on the Peplomer Protein of Coronaviruses

1990 • 12 citations

Expression of canine parvovirus-β-galactosidase fusion proteins in Escherichia coli

1984 • 12 citations

Location of Antigenic Sites of the S-Glycoprotein of Transmissible Gastroenteritis Virus and their Conservation in Coronaviruses

1990 • 11 citations

A Survey of Vectors for Regulating Expression of Cloned DNA in E. coli

1988 • 11 citations

Vaccines: New Concepts and Developments

1989 • 11 citations

High level expression of genes cloned in phage λgt11

1989 • 10 citations

[9] Use of open reading frame expression vectors

1987 • 9 citations

Measles virus-specific murine T cell clones: characterization of fine specificity and function.

1989 • 9 citations

An immunodominant domain in adenovirus type 2 early region 1A proteins

1986 • 9 citations

Purification of fusion proteins expressed by pEX3 and a truncated pEX3 derivative

1988 • 6 citations

Cloning and Expression of Viral Antigens in Escherichia coli and Other Microorganisms

1984 • 4 citations

Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli

1988 • 4 citations

Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli

1987 • 4 citations

[8] Searching for clones with open reading frames

1987 • 3 citations

Production of oncogene-specific proteins and human T-cell leukemia (lymphotropic) retrovirus (HTLV-I) envelope protein in bacteria and its potential for use in human cancers and seroepidemiological surveys.

1985 • 2 citations

pXmnATG: anE.colivector for expression of unfused proteins

1987 • 1 citations