Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites

1974 • 3,603 citations

Nucleotide sequence of bacteriophage G4 DNA

1978 • 2,137 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis

1981 • 1,881 citations

Nucleotide sequence of bacteriophage λ DNA

1982 • 1,171 citations

Synthesis of deoxyoligonucleotides on a polymer support

1981 • 870 citations

Cloning and expression of murine interleukin-1 cDNA in Escherichia coli

1984 • 820 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

[16] Plasmids of Escherichia coli as cloning vectors

1979 • 655 citations

A freeze-squeeze method for recovering long DNA from agarose gels

1975 • 478 citations

Effect of Growth Conditions on the Formation of the Relaxation Complex of Supercoiled ColE1 Deoxyribonucleic Acid and Protein in Escherichia coli

1972 • 351 citations

Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding

1982 • 348 citations

A role for mRNA secondary structure in the control of translation initiation

1982 • 344 citations

Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

1983 • 307 citations

A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.

1984 • 246 citations

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

1982 • 211 citations

Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans.

1983 • 211 citations

The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments

1980 • 206 citations

[6] The use of pKC30 and its derivatives for controlled expression of genes

1983 • 202 citations

[35] Use of the λ phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles

1979 • 127 citations

Increased Synthesis inE. coliof Fibroblast and Leukocyte Interferons Through Alterations in Ribosome Binding Sites

1982 • 106 citations

Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins.

1984 • 100 citations

HTLV-III env gene products synthesized in E. coli are recognized by antibodies present in the sera of AIDS patients

1985 • 81 citations

Stabilization of a degradable protein by its overexpression in Escherichia coli

1981 • 80 citations

Expression of Treponema pallidum Antigens in Escherichia coli

1982 • 72 citations

Portable Shine-Dalgarno Regions: A System for a Systematic Study of Defined Alterations of Nucleotide Sequences withinE. coliRibosome Binding Sites

1983 • 67 citations

[8] Plasmids containing the rp promoters of Escherichia coli and Serratia marcescents and their use in expressing cloned genes

1983 • 57 citations

Cloning, sequence analysis, and expression of alteration of the mRNA stability gene (ams+) of Escherichia coli

1985 • 55 citations

Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

1982 • 40 citations

Insulins, Growth Hormone, and Recombinant DNA Technology

1982 • 39 citations

Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site.

1981 • 38 citations

[55] Expression of human interferon genes in E. coli with the lambda PL promoter

1986 • 19 citations