High level expression of genes cloned in phage λgt11

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

Advanced bacterial genetics

1980 • 2,388 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

1977 • 711 citations

A sequence pattern common to T cell epitopes.

1988 • 623 citations

Easy identification of cDNA clones.

1983 • 587 citations

Inhibition of restriction endonuclease Nel I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis

1986 • 560 citations

[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

1987 • 392 citations

An improved filamentous helper phage for generating single-stranded plasmid DNA

1986 • 348 citations

Speeding-up the sequencing of double-stranded DNA

1988 • 133 citations

Mycobacterium leprae antigens involved in human immune responses. I. Identification of four antigens by monoclonal antibodies.

1985 • 97 citations

Construction of a family of universal expression plasmid vectors

1984 • 82 citations

Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli.

1986 • 36 citations

Recognition of mycobacterial antigens by sera from patients with leprosy

1988 • 23 citations