A Survey of Vectors for Regulating Expression of Cloned DNA in E. coli

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin

1977 • 829 citations

Attenuation in the control of expression of bacterial operons

1981 • 757 citations

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

1981 • 664 citations

Easy identification of cDNA clones.

1983 • 587 citations

Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

1984 • 538 citations

Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine

1981 • 504 citations

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

1984 • 374 citations

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.

1985 • 331 citations

Secretion cloning vectors in Escherichia coli.

1984 • 322 citations

Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors

1983 • 318 citations

Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

1983 • 307 citations

Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa.

1984 • 286 citations

New shuttle vectors for Bacillus subtilis and Escherichia coli which allow rapid detection of inserted fragments

1984 • 261 citations

Purified λ regulatory protein cII positively activates promoters for lysogenic development

1981 • 241 citations

Purification of Calf Prochymosin (Prorennin) Synthesized in Escherichia Coli

1984 • 227 citations

Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin

1980 • 227 citations

Construction and analysis of in vivo activity of E. coll promoter hybrids and promoter mutants that alter the −35 to −10 spacing

1982 • 219 citations

Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli.

1984 • 217 citations

Experimental Manipulation of Gene Expression

1983 • 213 citations

Bacterial expression and characterization of proteins derived from the chicken calmodulin cDNA and a calmodulin processed gene.

1985 • 209 citations

Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone

1985 • 201 citations

The product of the retroviral transforming gene v-myb is a truncated version of the protein encoded by the cellular oncogene c-myb

1983 • 197 citations

Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.

1985 • 184 citations

Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.

1984 • 159 citations

Human Cu/Zn superoxide dismutase cDNA: isolation of clones synthesising high levels of active or inactive enzyme from an expression library

1985 • 149 citations

Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes.

1984 • 149 citations

Efficient Bacterial Expression of Bovine and Porcine Growth Hormones

1983 • 144 citations

Optimizing the expression inE. coliof a synthetic gene encoding somatomedin-C (IGF-I)

1985 • 144 citations

Bacteria mature preproinsulin to proinsulin.

1980 • 143 citations

Synthesis of calf prochymosin (prorennin) in Escherichia coli.

1983 • 141 citations

Synthesis of hepatitis B surface and core antigens in E. coli. 1981.

1992 • 138 citations

Gene fusion vectors based on the gene for staphylococcal protein A

1983 • 137 citations

High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli.

1984 • 136 citations

Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

1984 • 131 citations

Construction of versatile expression cloning vehicles using the lipoprotein gene of Escherichia coli.

1982 • 129 citations

Synthesis of human insulin gene VIII. Construction of expression vectors for fused proinsulin production in Escherichia coli

1984 • 128 citations

Up-promoter mutations in thelppgene ofEscherichia coli

1985 • 126 citations

Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids

1980 • 117 citations

Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.

1985 • 117 citations

Novel High-level Expression Cloning Vehicles: 104-fold Amplification of Escherichia coli Minor Protein

1984 • 114 citations

Expression of the human apolipoprotein AI gene fused to the E. coli gene for β‐galactosidase

1986 • 113 citations

The product of the avian erythroblastosis virus erbB locus is a glycoprotein

1983 • 111 citations

Cloning, expression in Escherichia coli, and reconstitution of human myoglobin.

1985 • 111 citations

Adenovirus E1a Gene Product Expressed at High Levels in Escherichia coli Is Functional

1984 • 111 citations

Periplasmic production of correctly processed human growth hormone in Escherichia coli: natural and bacterial signal sequences are interchangeable

1985 • 110 citations

Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

1983 • 108 citations

Molecular cloning and nucleotide sequence of rat lingual lipase cDNA

1985 • 105 citations

High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda

1984 • 103 citations

Multiple joined genes prevent product degradation in Escherichia coli.

1984 • 102 citations

Inducible high level synthesis of mature human fibroblast interferon inEscherichia coli

1983 • 101 citations

Purification and Characterization of Human H-ras Proteins Expressed in Escherichia coli

1985 • 101 citations

Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins.

1984 • 100 citations

The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain inEscherichia coli

1984 • 98 citations

Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.

1983 • 93 citations

Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression

1985 • 90 citations

Amplification and purification of the bacteriophage Mu encoded B transposition protein.

1985 • 86 citations

Construction of a family of universal expression plasmid vectors

1984 • 82 citations

Chicken triosephosphate isomerase complements an Escherichia coli deficiency.

1985 • 76 citations

The replication initiator protein of plasmid R6K tagged with β-galactosidase shows sequence-specific DNA-binding

1983 • 73 citations

Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.

1982 • 70 citations

Bacterial expression of an enzymatically active protein encoded by RSV src gene

1982 • 66 citations

Expression of the PDGF-related transforming protein of simian sarcoma virus in E. coli

1984 • 65 citations

The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein.

1983 • 65 citations

Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain.

1984 • 64 citations

A convenient technique to compare the efficiency of promoters inEscherichia coli

1985 • 64 citations

Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli.

1981 • 61 citations

Control of cloned gene expression by promoter inversion in vivo: construction of the heat-pulseactivated att-nutL-p-att-N module

1985 • 60 citations

Secretion into the culture medium of a foreign gene product from Escherichia coli: use of the ompF gene for secretion of human beta-endorphin.

1985 • 59 citations

Immunoenzymatic detection of expressed gene fragments cloned in the lac Z gene of E. coli.

1982 • 59 citations

Biologically active protease of foot and mouth disease virus is expressed from cloned viral cDNA in Escherichia coli.

1984 • 59 citations

Photoaffinity labeling with GTP of viral p21 ras protein expressed in Escherichia coli

1984 • 57 citations

Cloning of the chick hsp 90 cDNA in expression vector

1985 • 56 citations

Effects of alterations in the translation control region on bacterial gene expression: use of cat gene constructs transcribed from the lac promoter as a model system

1984 • 54 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

Purification and characterization of human H-ras proteins expressed in Escherichia coli.

1985 • 53 citations

Bacteriophage lambda initiators: Preparation from a strain that overproduces the O and P proteins

1982 • 48 citations

Expression of human terminal deoxynucleotidyl transferase in Escherichia coli.

1985 • 43 citations

Expression of cloned calf prochymosin cDNA under control of the tryptophan promoter

1984 • 43 citations

Overproduction of theEcoR V endonuclease and methylase

1985 • 42 citations

Direct expression of hepatitis B surface antigen gene inE coli

1983 • 41 citations

Pseudomonas Aeruginosa Secretes and Correctly Processes Human Growth Hormone

1984 • 41 citations

An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS

1985 • 39 citations

Modification and processing of internalized signal sequences of prolipoprotein in Escherichia coli and in Bacillus subtilis.

1985 • 38 citations

Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoteroperator of Escherichia coli

1984 • 37 citations

High-level synthesis in Escherichia coli of the SV40 small-t antigen under control of the bacteriophage lambda pl promoter

1982 • 36 citations

Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli.

1986 • 36 citations

Construction and properties of plasmid pKC30, a pBR322 derivative containing the pl-N region of phage lambda

1984 • 36 citations

Expression of polyoma early gene products inE. coli

1985 • 33 citations

Overproduction of transcription termination factor Rho in Escherichia coli

1984 • 32 citations

Expression plasmid vectors containing Escherichia coli tryptophan promoter transcriptional units lacking the attenuator

1983 • 29 citations

Detection and characterization of a mouse alpha-spectrin cDNA clone by its expression in Escherichia coli.

1985 • 28 citations

Expression of a β-Lactamase Preproinsulin Fusion Protein in Escherichia Coli

1984 • 27 citations

Isolation and expression in Escherichia coli of a cDNA clone encoding human β-glucuronidase

1985 • 26 citations

Secretion and processing of an immunoglobulin light chain in Escherichia coli

1984 • 26 citations

Efficient Expression inEscherichia coliof a Mature and a Modified Human Interferon-β₁

1984 • 26 citations

Use of simian virus 40 large T-beta-galactosidase fusion proteins in an immunochemical analysis of simian virus 40 large T antigen

1985 • 25 citations

A direct-selection vector derived from pColE3-CA38 and adapted for foreign gene expression

1985 • 23 citations

Expression of the cloned bacteriophage phi X174 A* gene in Escherichia coli inhibits DNA replication and cell division

1985 • 23 citations

Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene.

1985 • 23 citations

High-level expression in Escherichia coli of calcium-binding domains of an embryonic sea urchin protein

1984 • 22 citations

Amplification of the uvrA gene product of Escherichia coli to 7% of cellular protein by linkage to the pL promoter of pKC30.

1982 • 22 citations

M13 bacteriophage and pUC plasmids containing DNA inserts but still capable of β-galactosidase α-complementation

1983 • 22 citations

Expression of FBJ-MSV oncogene (fos) product in bacteria

1983 • 19 citations

Plasmid vectors based on Tn10 DNA: Gene expression regulated by tetracycline

1984 • 18 citations

Themacpromoters: functional hybrid promoters activated by themalTproduct and repressed by thelacIproduct

1985 • 16 citations

Cloning and expression of gene 2, required for the protein-primed initiation of the Bacillus subtilis phage φ29 DNA replication

1984 • 15 citations

Expression of hepatitis B virus surface antigen gene in Escherichia coli

1984 • 14 citations

Cloning and expression of the phage Mu A gene

1984 • 13 citations

Functional mutants of phenylalanine transfer RNA from Escherichia coli.

1985 • 12 citations

Regulated high-level expression of the Herpes simplex type I thymidine kinase gene in Escherichia coli

1984 • 9 citations

Expression of immunogenically reactive diphtheria toxin fusion proteins under the control of the pR promoter of bacteriophage lambda

1986 • 8 citations

Expression in Escherichia coli of a fusion protein product containing a region of the adenovirus DNA polymerase.

1985 • 7 citations

Expression of antigenic regions of a trypanosome variable surface glycoprotein inE. coliusing Bal-31 nuclease digestion

1984 • 3 citations

Deleted Work

1955 • 0 citations