The Yeast KEX-2-Processing Endoprotease Is Active in the Golgi Apparatus of Transfected NIH 3T3 Fibroblasts

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A simple salting out procedure for extracting DNA from human nucleated cells

1988 • 20,341 citations

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

A C-terminal signal prevents secretion of luminal ER proteins

1987 • 2,189 citations

Common precursor to corticotropins and endorphins

1977 • 1,106 citations

A ras-like protein is required for a post-Golgi event in yeast secretion

1987 • 894 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine Peptides

1984 • 497 citations

Enzymes Required for Yeast Prohormone Processing

1988 • 446 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles

1987 • 335 citations

Sorting of soluble ER proteins in yeast.

1988 • 270 citations

Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases

1988 • 236 citations

Secretion and processing of insulin precursors in yeast.

1986 • 227 citations

Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and α-factor precursor processing

1987 • 183 citations

Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone in Mammalian Cells

1988 • 173 citations

Proteolytic Processing of Pro-ACTH/Endorphin Begins in the Golgi Complex of Pituitary Corticotropes and AtT-20 Cells

1989 • 165 citations

Clathrin: A Role in the Intracellular Retention of a Golgi Membrane Protein

1989 • 154 citations

Synthesis of human insulin gene VIII. Construction of expression vectors for fused proinsulin production in Escherichia coli

1984 • 128 citations

Conversion of a secretory protein into a transmembrane protein results in its transport to the golgi complex but not to the cell surface

1984 • 120 citations

Complete structure of the porcine pro-opiomelanocortin mRNA derived from the nucleotide sequence of doned cDNA

1983 • 92 citations

Yeast KEX2 protease and mannosyltransferase I are localized to distinct compartments of the secretory pathway

1989 • 81 citations

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.

1984 • 80 citations

Proteolytic conversion of proinsulin into insulin. Identification of a Ca2+-dependent acidic endopeptidase in isolated insulin-secretory granules

1987 • 69 citations

A novel serine protease (IRCM-serine protease 1) from porcine neurointermediate and anterior pituitary lobes. Isolation, polypeptide chain structure, inhibitor sensitivity, and substrate specificity with fluorogenic peptide substrates.

1986 • 55 citations

Site-specific mutagenesis identifies amino acid residues critical in prohormone processing.

1989 • 54 citations

Yeast and mammals utilize similar cytosolic components to drive protein transport through the Golgi complex.

1986 • 52 citations

Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving prooxytocin/neurophysin peptides at the basic amino acid doublet

1987 • 44 citations

Expression of porcine pro-opiomelanocortin cDNA in heterologous monkey kidney cells. Biosynthesis and secretion of the prohormone without processing.

1987 • 37 citations

Kinetic studies on the processing of human beta-lipotropin by bovine pituitary intermediate lobe pro-opiomelanocortin-converting enzyme.

1986 • 36 citations

Targeting and Processing of Pro‐Opiomelanocortin in Neuronal Cell Lines

1989 • 36 citations

Secretion of somatostatin by Saccharomyces cerevisiae. Correct proteolytic processing of pro-alpha-factor-somatostatin hybrids requires the products of the KEX2 and STE13 genes.

1988 • 33 citations

Identification of the structural gene for dipeptidyl aminopeptidase yscV (DAP2) of Saccharomyces cerevisiae

1987 • 31 citations

Relationship between endo- and exopeptidases in a processing enzyme system: activation of an endoprotease by the aminopeptidase B-like activity in somatostatin-28 convertase.

1988 • 30 citations

Intracellular proteolytic processing of proopiomelanocortin in heterologous COS-1 cells by the yeast KEX2 endoprotease

1990 • 15 citations

Expression of porcine pro-opiomelanocortin cDNA in an established fibroblastic cell line: constitutive secretion of the precursor without proteolytic processing

1988 • 7 citations